Purification of Bacteriophage λ Particles by Isopycnic Centrifugation through CsCl Gradients

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot3968 ◽  
Author(s):  
Joseph Sambrook ◽  
David W. Russell
Keyword(s):  
Bacteriophage Λ ◽  
Isopycnic Centrifugation

Q-mediated late gene transcription of bacteriophage λ: RNA start point and RNase III processing sites in vivo

Virology ◽  
1988 ◽  
Vol 167 (2) ◽  
pp. 568-577 ◽  
Author(s):  
D DANIELS ◽  
M SUBBARAO ◽  
F BLATTNER ◽  
H LOZERON
Keyword(s):  
Gene Transcription ◽  
Late Gene ◽  
Bacteriophage Λ ◽  
Rnase Iii

Restriction of bacteriophage λ by Escherichia coli K

1973 ◽  
Vol 81 (3) ◽  
pp. 395-407 ◽  
Author(s):  
Noreen E. Murray ◽  
P.L. Batten ◽  
K. Murray
Keyword(s):  
Escherichia Coli ◽  
Bacteriophage Λ

Genetic analysis of the tof gene in the bacteriophage λ genome

Virology ◽  
1975 ◽  
Vol 65 (2) ◽  
pp. 385-391 ◽  
Author(s):  
Y. Takeda ◽  
K. Ogata ◽  
K. Matsubara
Keyword(s):  
Genetic Analysis ◽  
Bacteriophage Λ

scholarly journals VERY SHORT PATCH MISMATCH REPAIR IN PHAGE LAMBDA: REPAIR SITES AND LENGTH OF REPAIR TRACTS

Genetics ◽  
1986 ◽  
Vol 114 (4) ◽  
pp. 1041-1060
Author(s):  
M Lieb ◽  
E Allen ◽  
D Read
Keyword(s):  
Mismatch Repair ◽  
Bacteriophage Λ ◽  
Phage Lambda ◽  
High Frequencies ◽  
Vsp Repair

ABSTRACT Five amber mutations in the repressor (cl) gene of bacteriophage λ recombine anomalously with nearby cl mutations. When any of these markers is used in four-factor crosses, cl  + recombinants that are expected to require three crossovers occur at high frequencies. These recombinants are attributable to very-short-patch (VSP) repair of specific mismatches in DNA heteroduplexes formed during recombination between the markers flanking cl. The sites of the repairprone mutations and the lengths of repair tracts have now been determined. Amber mutations subject to VSP repair are C to T transitions in (see PDF), the sequence methylated by the product of gene dcm, and also in the related 5′CAGG or 5′CCAG sequences. Ambers arising in CAG sequences found in other contexts, or in codons other than CAG, were not subject to VSP repair. Repair tracts rarely, if ever, exceed ten nucleotides in length, and can be as short as two nucleotides. A repair-prone mutation does not stimulate recombination between flanking cl markers.

scholarly journals THE INTERACTION OF cos WITH CHI IS SEPARABLE FROM DNA PACKAGING IN recA-recBC-MEDIATED RECOMBINATION OF BACTERIOPHAGE LAMBDA

Genetics ◽  
1983 ◽  
Vol 104 (4) ◽  
pp. 549-570
Author(s):  
Ichizo Kobayashi ◽  
Mary M Stahl ◽  
David Leach ◽  
Franklin W Stahl
Keyword(s):  
Escherichia Coli ◽  
Bacteriophage Lambda ◽  
Dna Packaging ◽  
Bacteriophage Λ ◽  
Entry Site ◽  
Two Component ◽  
General Recombination

ABSTRACT Chi (5′-GCTGGTGG) is a recombinator in RecA- RecBC-mediated recombination in Escherichia coli. In bacteriophage λ vegetative recombination, Chi is fully active only when it is correctly oriented with respect to cos, the site that defines the ends of the packaged chromosome. Here we demonstrate that packaging from cos is not necessary for this cos-Chi interaction. Our evidence suggests that correctly oriented cos is an activator of Chi. cos, as an activator, is (1) dominant over cos  -, (2) active opposite an extensive heterology, (3) able to interact with Chi only when on the same (cis) chromosome, and (4) able to interact with Chi at distances as far as ≥ 20 kb. Thus, cos and Chi form a two-component recombinator system for general recombination. cos may serve as an asymmetric entry site for a recombination enzyme that recognizes Chi in an asymmetric way.

scholarly journals GENETIC HETEROZYGOSITY IN UNREPLICATED BACTERIOPHAGE Λ RECOMBINANTS

Genetics ◽  
1975 ◽  
Vol 81 (1) ◽  
pp. 33-50
Author(s):  
Raymond L White ◽  
Maurice S Fox
Keyword(s):  
Dna Synthesis ◽  
Base Pair ◽  
Recombinant Dna ◽  
Dna Molecules ◽  
Bacteriophage Λ ◽  
Negative Interference ◽  
Genetic Interval ◽  
Parental Material ◽  
Genetic Heterozygosity ◽  
Genetic Contributions

ABSTRACT Bacteriophage crosses using density-labeled parents have been carried out under conditions restricting DNA synthesis. The parental material and genetic contributions to progeny manifesting recombination within a genetic interval sufficiently short to exhibit high negative interference have been examined. The unreplicated products of recombination isolated as phage particles appear to contain long continuous heteroduplex regions which are heterozygous for the closely linked markers. Recombination between closely linked markers seems to be the consequence of the removal of base-pair mismatches that are present within the heteroduplex regions. This localized reduction of heterozygosity within the heteroduplex regions that join the parental components of recombinant DNA molecules can account for high negative interference.

Structure of the oligomeric circular molecules of a bacteriophage λ DNA

Virology ◽  
1975 ◽  
Vol 64 (2) ◽  
pp. 319-329 ◽  
Author(s):  
Seishi Takahashi
Keyword(s):  
Bacteriophage Λ
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