Total RNA Extraction from Caenorhabditis elegans

2020 ◽  
Vol 2020 (9) ◽  
pp. pdb.prot101683
Author(s):  
Michael R. Green ◽  
Joseph Sambrook
Keyword(s):  
Caenorhabditis Elegans ◽  
Rna Extraction ◽  
Total Rna ◽  
Total Rna Extraction

A RAPID AND EFFECTIVE METHOD FOR TOTAL RNA EXTRACTION FROM CONIDIA AND MYCELIUM OF SEPTORIA TRITICI

2003 ◽  
Vol 11 (1) ◽  
pp. 53-59 ◽  
Author(s):  
JIAN-RONG GUO ◽  
KAMEL A. ABD-ELSALAM ◽  
FRANK SCHNIEDER ◽  
JOSEPH-ALEXANDER VERREET
Keyword(s):  
Rna Extraction ◽  
Septoria Tritici ◽  
Total Rna ◽  
Total Rna Extraction

scholarly journals Comparison of different methods for total RNA extraction from sclerotia of Rhizoctonia solani

2014 ◽  
Vol 17 (1) ◽  
pp. 50-54 ◽  
Author(s):  
Canwei Shu ◽  
Si Sun ◽  
Jieling Chen ◽  
Jianyi Chen ◽  
Erxun Zhou
Keyword(s):  
Rhizoctonia Solani ◽  
Rna Extraction ◽  
Total Rna ◽  
Total Rna Extraction

scholarly journals An efficient method for total RNA extraction from leaves of arboreal species from the Brazilian Cerrado

Rodriguésia ◽  
2020 ◽  
Vol 71 ◽  
Author(s):  
Geisiane Alves Rocha ◽  
Vanessa Duarte Dias ◽  
Renato Carrer-Filho ◽  
Marcos Gomes da Cunha ◽  
Érico de Campos Dianese
Keyword(s):  
Native Species ◽  
Rna Extraction ◽  
Woody Species ◽  
Extraction Methods ◽  
Native Plant ◽  
Brazilian Cerrado ◽  
Native Plant Species ◽  
Total Rna ◽  
Ctab Method ◽  
Total Rna Extraction

Abstract Considering the lack of information on RNA extraction from arboreal species, specially from the Brazilian Cerrado, the aim of this study was to test RNA extraction methods for a wide variety of native plant species from this biome. The methods tested consisted of: (i) TRIzol® reagent, (ii) TRIzol® reagent with modifications, (iii) CTAB buffer, and (iv) Modified CTAB buffer, initially for leaf samples of Xylopia aromatica and Piper arboreum. Later the procedure with the best results was used to obtain purified RNA from 17 other native species. Based on A260/A280 absorbance ratio the Modified CTAB method was the best for total RNA extraction for those woody species. Ten out of eleven species tested through RT-PCR generated fragments of the expected size from the total RNA extracted by the selected method, confirming it as the best option to obtain high-quality RNA for molecular analyses and for use in the detection of viruses infecting these tree species.

scholarly journals All in One High Quality Genomic DNA and Total RNA Extraction From Nematode Induced Galls for High Throughput Sequencing Purposes

2019 ◽  
Vol 10 ◽  
Author(s):  
Ana Cláudia Silva ◽  
Virginia Ruiz-Ferrer ◽  
Ángela Martínez-Gómez ◽  
Marta Barcala ◽  
Carmen Fenoll ◽  
...  
Keyword(s):  
High Throughput ◽  
Genomic Dna ◽  
High Throughput Sequencing ◽  
Rna Extraction ◽  
High Quality ◽  
Total Rna ◽  
Total Rna Extraction

Evaluation of U.S. Food and Drug Administration Enteric Viruses Microarray for Detection of Hepatitis A Virus and Norovirus in Inoculated Tomatoes, Green Onions, and Celery

2020 ◽  
Vol 83 (9) ◽  
pp. 1576-1583
Author(s):  
CHRISTINE YU ◽  
KAORU HIDA ◽  
EFSTATHIA PAPAFRAGKOU ◽  
MICHAEL KULKA
Keyword(s):  
Drug Administration ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Limit Of Detection ◽  
Food And Drug Administration ◽  
Fresh Produce ◽  
Rna Extraction ◽  
Enteric Viruses ◽  
Total Rna ◽  
Total Rna Extraction

ABSTRACT Foodborne viral contamination of fresh produce has been associated with numerous outbreaks. Detection of such contaminated foods is important in protecting public health. Here, we demonstrate for the first time the capability of the U.S. Food and Drug Administration Enteric Viruses tiling microarray (FDA-EVIR) to perform rapid molecular identification of hepatitis A virus (HAV) and human norovirus extracted from artificially inoculated fresh produce. Two published viral extraction strategies, total RNA extraction or virus particle isolation, were used to prepare the viral targets. The total RNA extraction method was used on material eluted from tomatoes, using an alkaline Tris–glycine–beef extract (TGBE) buffer. Optimization procedures including DNase treatment and poly(A)-RNA enrichment were adopted to improve microarray sensitivity. For green onions or celery, material was eluted using either glycine buffer or TGBE buffer supplemented with pectinase, respectively, and then virus particles were concentrated by ultracentrifugation. We also assessed the amount of viral RNA extracted from celery using three commercially available kits and how well that RNA performed on FDA-EVIR. Our results confirm that FDA-EVIR can identify common enteric viruses isolated from fresh produce when present as either a single or mixed species of viruses. Using total RNA extraction from tomatoes yielded a limit of detection of 1.0 × 105 genome equivalents (ge) of HAV per array input. The limit of detection for viral RNA obtained using ultracentrifugation was 1.2 × 105 ge of HAV from green onions and 1.0 × 103 ge of norovirus from celery per array input. Extending microarray methods to other food matrices should provide important support to surveillance and outbreak investigations. HIGHLIGHTS

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