Induction of Ascites in Mice

2021 ◽  
Vol 2021 (10) ◽  
pp. pdb.prot100289
Author(s):  
Edward A. Greenfield
Keyword(s):  
Polyclonal Antibody ◽  
Antibody Production ◽  
Guinea Pigs ◽  
Ascites Fluid ◽  
Single Animal ◽  
Antibody Titers

Smaller animals such as rats, mice, hamsters, and guinea pigs are usually poor choices for polyclonal antibody production because only small volumes of serum can be obtained. This problem can be reduced by inducing the formation of ascites in mice, which can provide up to 10 mL of ascites fluid from a single animal. Antibody titers in ascites fluids are almost as high as serum titers.

Immunology of the inner ear: Response of the inner ear to antigen challenge

1983 ◽  
Vol 91 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Jeffrey P. Harris
Keyword(s):  
Inner Ear ◽  
Blood Brain Barrier ◽  
Guinea Pigs ◽  
Keyhole Limpet Hemocyanin ◽  
Host Immunity ◽  
Brain Barrier ◽  
Antigen Challenge ◽  
Antibody Titers ◽  
Relationship Of ◽  
The Relationship

The relationship of the inner ear to host immunity and the immunoresponsiveness of the inner ear to antigen challenge were investigated. A radioimmunoassay was used to quantitate antibody titers to keyhole-limpet hemocyanin generated in the serum, perilymph, and CSF of guinea pigs following systemic or inner ear immunizations. The results of these experiments demonstrate (1) the blood-labyrinth barrier is analogous to the blood-brain barrier with respect to immunoglobulin equilibrium, (2) the inner ear is capable of responding to antigen challenge, and (3) the inner ear is an effective route for systemic immunization.

scholarly journals Evolution of anti-Trypanosoma cruzi antibody production in patients with chronic Chagas disease: Correlation between antibody titers and development of cardiac disease severity

2017 ◽  
Vol 11 (7) ◽  
pp. e0005796 ◽  
Author(s):  
Ingebourg Georg ◽  
Alejandro Marcel Hasslocher-Moreno ◽  
Sergio Salles Xavier ◽  
Marcelo Teixeira de Holanda ◽  
Eric Henrique Roma ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Disease Severity ◽  
Chagas Disease ◽  
Antibody Production ◽  
Cardiac Disease ◽  
Antibody Titers ◽  
Chronic Chagas Disease

scholarly journals Spatial requirements between haptenic and carrier determinants for T-dependent antibody responses.

1978 ◽  
Vol 148 (3) ◽  
pp. 817-822 ◽  
Author(s):  
S Fong ◽  
D E Nitecki ◽  
R M Cook ◽  
J W Goodman
Keyword(s):  
B Cells ◽  
Antibody Production ◽  
Guinea Pigs ◽  
Effective Interaction ◽  
Antibody Responses ◽  
T And B Cells ◽  
Axial Translation ◽  
Bifunctional Compounds

To gauge the proximity between cooperating T and B cells required for effective triggering of antibody production, guinea pigs were immunized with bifunctional antigens in which the haptenic and carrier determinants were separated by rigid spacers of varied dimension. These took the form 2,4-dinitrophenol-(proline)n-L-tyrosine-p-azobenzenearsonate, where n varied from 1 to 40 proline residues. Animals immunized with n = 10 and n = 22 compounds made strong anti-DNP antibody responses, whereas animals immunized with bifunctional compounds containing longer spacers did not make antibody detectable by precipitation. It can be calculated on the basis of very strong physicochemical evidence for the rigidity and axial translation of poly-L-proline chains in solution that the cut-off point for effective interaction between T and B cells lies between 69 and 97 A U.

scholarly journals Prokaryotic expression, purification, and polyclonal antibody production of a hydrophobin from Grifola frondosa

2010 ◽  
Vol 42 (6) ◽  
pp. 388-395 ◽  
Author(s):  
Z. Wang ◽  
S. Feng ◽  
Y. Huang ◽  
M. Qiao ◽  
B. Zhang ◽  
...  
Keyword(s):  
Polyclonal Antibody ◽  
Antibody Production ◽  
Prokaryotic Expression ◽  
Grifola Frondosa

scholarly journals Species-Specific Chromosome Engineering Greatly Improves Fully Human Polyclonal Antibody Production Profile in Cattle

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0130699 ◽  
Author(s):  
Hiroaki Matsushita ◽  
Akiko Sano ◽  
Hua Wu ◽  
Zhongde Wang ◽  
Jin-an Jiao ◽  
...  
Keyword(s):  
Polyclonal Antibody ◽  
Antibody Production ◽  
Chromosome Engineering ◽  
Specific Chromosome ◽  
Species Specific
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