scholarly journals Lymphocyte Deficiency Induced by Sublethal Irradiation in Xenopus

2018 ◽  
Vol 2019 (1) ◽  
pp. pdb.prot097626 ◽  
Author(s):  
Louise A. Rollins-Smith ◽  
Jacques Robert
Keyword(s):  
Sublethal Irradiation

Protection against influenza virus infection by nasal vaccination in advance of sublethal irradiation

Vaccine ◽  
2000 ◽  
Vol 18 (23) ◽  
pp. 2560-2565 ◽  
Author(s):  
Hideki Hasegawa ◽  
Shin-etsu Kadowaki ◽  
Hidehiro Takahashi ◽  
Takuya Iwasaki ◽  
Shin-ichi Tamura ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Infection ◽  
Influenza Virus Infection ◽  
Nasal Vaccination ◽  
Sublethal Irradiation

scholarly journals β-Glucan enhances complement-mediated hematopoietic recovery after bone marrow injury

Blood ◽  
2006 ◽  
Vol 107 (2) ◽  
pp. 835-840 ◽  
Author(s):  
Daniel E. Cramer ◽  
Daniel J. Allendorf ◽  
Jarek T. Baran ◽  
Richard Hansen ◽  
Jose Marroquin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mouse Serum ◽  
Complement Receptor ◽  
Dependent Manner ◽  
Complement Receptor 3 ◽  
Total Body ◽  
Stimulating Factor ◽  
Sublethal Irradiation ◽  
Classic Pathway

AbstractMyelotoxic injury in the bone marrow (BM) as a consequence of total body irradiation (TBI) or granulocyte colony-stimulating factor (G-CSF) mobilization results in the deposition of iC3b on BM stroma (stroma-iC3b). In the present study, we have examined how stroma-iC3b interacts with hematopoietic progenitor cells (HPCs) and the role of complement (C) and complement receptor 3 (CR3) in BM injury/repair. We demonstrate here that stroma-iC3b tethers HPCs via the inserted (I) domain of HPC complement receptor 3 (CR3, CD11b/CD18, Mac-1). Following irradiation, stroma-iC3b was observed in the presence of purified IgM and normal mouse serum (NMS), but not serum from Rag-2-/- mice, implicating a role for antibody (Ab) and the classic pathway of C activation. Furthermore, a novel role for soluble yeast β-glucan, a ligand for the CR3 lectin-like domain (LLD), in the priming of CR3+ HPC is suggested. Soluble yeast β-glucan could enhance the proliferation of tethered HPCs, promote leukocyte recovery following sublethal irradiation, and increase the survival of lethally irradiated animals following allogeneic HPC transplantation in a CR3-dependent manner. Taken together, these observations suggest a novel role for C, CR3, and β-glucan in the restoration of hematopoiesis following injury. (Blood. 2006;107:835-840)

scholarly journals Syngeneic transplantation of hematopoietic stem cells that are genetically modified to express factor VIII in platelets restores hemostasis to hemophilia A mice with preexisting FVIII immunity

Blood ◽  
2008 ◽  
Vol 112 (7) ◽  
pp. 2713-2721 ◽  
Author(s):  
Qizhen Shi ◽  
Scot A. Fahs ◽  
David A. Wilcox ◽  
Erin L. Kuether ◽  
Patricia A. Morateck ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Transgenic Mice ◽  
Hematopoietic Stem Cells ◽  
Factor Viii ◽  
Hemophilia A ◽  
Genetically Modified ◽  
Hematopoietic Stem ◽  
Inhibitory Antibodies ◽  
Sublethal Irradiation

Abstract Although genetic induction of factor VIII (FVIII) expression in platelets can restore hemostasis in hemophilia A mice, this approach has not been studied in the clinical setting of preexisting FVIII inhibitory antibodies to determine whether such antibodies would affect therapeutic engraftment. We generated a line of transgenic mice (2bF8) that express FVIII only in platelets using the platelet-specific αIIb promoter and bred this 2bF8 transgene into a FVIIInull background. Bone marrow (BM) from heterozygous 2bF8 transgenic (2bF8tg+/−) mice was transplanted into immunized FVIIInull mice after lethal or sublethal irradiation. After BM reconstitution, 85% of recipients survived tail clipping when the 1100-cGy (myeloablative) regimen was used, 85.7% of recipients survived when 660-cGy (nonmyeloablative) regimens were used, and 60% of recipients survived when the recipients were conditioned with 440 cGy. Our further studies showed that transplantation with 1% to 5% 2bF8tg+/− BM cells still improved hemostasis in hemophilia A mice with inhibitors. These results demonstrate that the presence of FVIII-specific immunity in recipients does not negate engraftment of 2bF8 genetically modified hematopoietic stem cells, and transplantation of these hematopoietic stem cells can efficiently restore hemostasis to hemophilic mice with preexisting inhibitory antibodies under either myeloablative or nonmyeloablative regimens.

A Study of Some Metabolic Requirements for Repair of Sublethal Irradiation Damage

Radiology ◽  
1969 ◽  
Vol 93 (4) ◽  
pp. 895-899 ◽  
Author(s):  
Regina Roux Ashby ◽  
Frederick J. Bonte ◽  
James A. Belli
Keyword(s):  
Irradiation Damage ◽  
Sublethal Irradiation

scholarly journals Pegylated megakaryocyte growth and development factor abrogates the lethal thrombocytopenia associated with carboplatin and irradiation in mice

Blood ◽  
1995 ◽  
Vol 86 (12) ◽  
pp. 4486-4492 ◽  
Author(s):  
MM Hokom ◽  
D Lacey ◽  
OB Kinstler ◽  
E Choi ◽  
S Kaufman ◽  
...  
Keyword(s):  
Growth And Development ◽  
Granulocyte Colony Stimulating Factor ◽  
Potent Inducer ◽  
Development Factor ◽  
Internal Bleeding ◽  
Life Threatening ◽  
Stimulating Factor ◽  
Sublethal Irradiation

Megakaryocyte growth and development factor (MGDF) is a potent inducer of megakaryopoiesis in vitro and thrombopoiesis in vivo. The effects of MGDF appear to be lineage-selective, making this cytokine an ideal candidate for use in alleviating clinically relevant thrombocytopenias. This report describes a murine model of life-threatening thrombocytopenia that results from the combination treatment of carboplatin and sublethal irradiation. Mortality of this regimen is 94% and is associated with widespread internal bleeding. The daily administration of pegylated recombinant human MGDF (PEG-rMGDF) significantly reduced mortality (to < 15%) and ameliorated the depth and duration of thrombocytopenia. The severity of leucopenia and anemia was also reduced, although it was not clear whether these effects were direct. Platelets generated in response to PEG-rMGDF were morphologically indistinguishable from normal platelets. PEG-rMGDF administered in combination with murine granulocyte colony-stimulating factor completely prevented mortality and further reduced leukopenia and thrombocytopenia. These data support the concept that PEG-rMGDF may be useful to treat iatrogenic thrombocytopenias.

PIXE ANALYSIS OF THE SERUM OF MICE ACQUIRED RADIORESISTANCE AFTER SMALL DOSE OF X-RAY IRRADIATION

1996 ◽  
Vol 06 (03n04) ◽  
pp. 523-530 ◽  
Author(s):  
M. YONEZAWA ◽  
Y. MATSUDA ◽  
F. NISHIYAMA
Keyword(s):  
Blood Cell ◽  
Common Knowledge ◽  
X Rays ◽  
Defence Mechanisms ◽  
Pixe Analysis ◽  
X Ray ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
The Common ◽  
Sublethal Irradiation

It is important for maintenance and understanding of health to make inquiries into the biological defence mechanisms. Yonezawa et al. came across an induction of yet unknown defence mechanisms in mice which acquired two types of radioresistance (decrease in bone-marrow death rate after midlethal exposure): one occured 2 weeks after preirradiation with 0.3–0.5 Gy and the other 2 months after 0.05–0.10 Gy of X-rays. To elucidate the acquired radioresistance induced after preirradiation with 0.5 Gy (in a shorter time case). recovery of blood cell counts after sublethal irradiation were measured in mice of ICR strain. Contrary to the common knowledge on radiation protection. recovery of blood cell counts of thrombocytes. leukocytes and erythrocytes after sublethal irradiation were not stimulated by the preirradiation. This study was planned to find some keys to elucidate the mechanism for the acquired radioresistance. Eleven elements. Cl. K. Ca. Cr. Mn. Fe. Ni. Cu. Zn. Se and Br. were analysed clearly in mice sera by PIXE.

scholarly journals Murine recombinant interleukin-3 induces recovery of T and B cells in irradiated mice

Blood ◽  
1994 ◽  
Vol 83 (6) ◽  
pp. 1563-1568 ◽  
Author(s):  
D Frasca ◽  
G Leter ◽  
G Doria
Keyword(s):  
B Cell ◽  
Cell Number ◽  
Con A ◽  
Double Positive ◽  
Cd8 Cells ◽  
Interleukin 3 ◽  
Cell Functions ◽  
Cell Counts ◽  
Single Positive ◽  
Sublethal Irradiation

Abstract Injection of murine recombinant interleukin-3 (IL-3) into (C57BL/10 x DBA/2)F1 mice, sublethally irradiated with 300 cGy and killed 14 days later, induced in the thymus recovery of the cell number and mitotic responsiveness to concanavalin A (Con A), as well as an increase in number of double-negative CD4-CD8-, double-positive CD4+ CD8+, and single-positive CD4+CD8- and CD4-CD8+ cells. Also in the spleen, the cell count and mitotic responsiveness to Con A and lipopolysaccharide were increased to normal levels by IL-3 treatment. If the assays were performed 21 or 28 days after irradiation, IL-3 treatment was able to restore thymus and spleen cell counts as well as T- and B-cell mitotic responsiveness, even when mice were exposed to 400 or 500 cGy, respectively. These results altogether indicate that IL-3 induces differentiation and growth of thymocytes and recovery of T- and B-cell functions in mice exposed to sublethal irradiation.

scholarly journals S1.02 * SUBLETHAL IRRADIATION ENHANCES INVASIVENESS OF MALIGNANT GLIOMA CELLS; IMPLICATIONS FOR RADIOTHERAPY OF HUMAN GLIOBLASTOMA

2014 ◽  
Vol 16 (suppl 2) ◽  
pp. ii25-ii26
Author(s):  
S. Jung ◽  
J. Pei ◽  
H. H. Ryu ◽  
I. H. Park ◽  
M. Wen ◽  
...  
Keyword(s):  
Malignant Glioma ◽  
Glioma Cells ◽  
Human Glioblastoma ◽  
Malignant Glioma Cells ◽  
Sublethal Irradiation
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