Mouse Embryo Cryopreservation by Rapid Cooling

2018 ◽  
Vol 2018 (5) ◽  
pp. pdb.prot094557 ◽  
Author(s):  
Jillian Shaw
Keyword(s):  
Mouse Embryo ◽  
Embryo Cryopreservation ◽  
Rapid Cooling

Mouse Embryo Cryopreservation by Slow Freezing

2018 ◽  
Vol 2018 (5) ◽  
pp. pdb.prot094540 ◽  
Author(s):  
Robert Taft
Keyword(s):  
Mouse Embryo ◽  
Slow Freezing ◽  
Embryo Cryopreservation

scholarly journals A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability

Reproduction ◽  
1990 ◽  
Vol 89 (1) ◽  
pp. 91-97 ◽  
Author(s):  
M. Kasai ◽  
J. H. Komi ◽  
A. Takakamo ◽  
H. Tsudera ◽  
T. Sakurai ◽  
...  
Keyword(s):  
Mouse Embryo ◽  
Embryo Cryopreservation ◽  
Simple Method ◽  
Low Toxicity ◽  
Vitrification Solution

scholarly journals Mouse embryo cryopreservation utilizing a novel high-capacity vitrification spatula

BioTechniques ◽  
2009 ◽  
Vol 46 (7) ◽  
pp. 550-552 ◽  
Author(s):  
Wai Hung Tsang ◽  
King L. Chow
Keyword(s):  
Mouse Embryo ◽  
High Capacity ◽  
Embryo Cryopreservation

Titration of vitrification solution in mouse embryo cryopreservation

Cryobiology ◽  
1988 ◽  
Vol 25 (6) ◽  
pp. 567 ◽  
Author(s):  
A. Arav ◽  
L. Gianaroli ◽  
P. Suriano
Keyword(s):  
Mouse Embryo ◽  
Embryo Cryopreservation ◽  
Vitrification Solution

117 VITRIFICATION OF CHAROLAIS AND HOLSTEIN BLASTOCYSTS USING LN SLUSH

2006 ◽  
Vol 18 (2) ◽  
pp. 167
Author(s):  
S. Yavin ◽  
D. Ditesheim ◽  
Y. Zeron ◽  
A. Arav
Keyword(s):  
Ethylene Glycol ◽  
Cooling Rate ◽  
Simple Technique ◽  
Embryo Cryopreservation ◽  
Minimum Volume ◽  
Rapid Cooling ◽  
Suggested Procedure ◽  
Recipient Age ◽  
Vitrification Solution ◽  
Healthy Calf

Cryopreservation of embryos for transfer in outdoor conditions is a challenge; therefore a rapid and simple technique should be applied. Two different experiments were performed to develop a functional and efficient vitrification technique. Charolais (for Exp. 1) and Holstein (for Exp. 2) female cows were superovulated and artificially inseminated (AI). Seven days after AI, embryos were flushed from the uterus and vitrified. For vitrification, blastocyst were exposed to 10% vitrification solution (VS) for 3 min, transferred into 50% VS, and immediately thereafter into 87.5% VS (100% VS containing 38% (v/v) ethylene glycol (EG), 0.5 m trehalose, and 6% BSA in PBS). The embryos were then loaded into super open pulled straws (SOPS) (Minitub, Tiefenbach, Germany) in a minimum-volume droplet (0.5 μL). The SOPSs containing the embryos were sealed by applying a soldering device to the narrow end and by inserting an identification rod into the wide end. Samples were vitrified at a rapid cooling rate in LN Slush (VitMaster apparatus, IMT Ltd, Ness-Ziona, Israel). On the day of transfer, blastocysts were warmed by plunging the SOPS into the warming chamber of the device, which contained 70% ethanol at 37°C, for 5 s. The straws were then withdrawn from the warming chamber and the sealed end was cut off carefully. The embryos were immersed in a 200-μL drop of 0.6 m trehalose in PBS solution for 4 min and then transferred through a series of solutions containing decreasing concentrations (0.5, 0.4, 0.3, 0.2, and 0.1 m) of trehalose for 2 min each. Viability was evaluated according to the ability of the embryos to re-expand. Blastocysts with the highest morphology rank were selected for transfer and loaded into the transfer gun (Minitub). Holstein recipients were in estrus 6–8 days prior to transfer. In Exp. 1, all blastocysts (n = 15) re-expanded after the vitrification and warming processes and were transferred into the uteruses of 15 Holstein recipient cows. One recipient cow became pregnant and gave birth to a healthy calf. In Exp. 2, all blastocysts (n = 6) re-expanded after warming and were transferred, one each into the uterus of a recipient Holstein heifer. Three pregnancies are still ongoing. In summary, the results obtained demonstrate that plunging embryos in SOPSs into LN Slush in outdoor conditions offers a potential technique for embryo cryopreservation and transfers. Further field trails are required to examine the effect of recipient age (cow vs. heifer) and embryo breed (Charolais vs. Holstein) on the suggested procedure.

Spontaneous Production of Type C Virus Particles in a Culture Derived from Rat Embryo Cells

1972 ◽  
Vol 30 ◽  
pp. 288-289
Author(s):  
Elizabeth S. Priori ◽  
T. Shigematsu ◽  
B. Myers ◽  
L. Dmochowski
Keyword(s):  
Virus Particle ◽  
Mouse Embryo ◽  
Calf Serum ◽  
Embryo Cell ◽  
Virus Particles ◽  
Extracellular Virus ◽  
Mouse Embryo Cell ◽  
Mature Virus Particle ◽  
Embryo Cells ◽  
Type C

Spontaneous release of type C virus particles in long-term cultures of mouse embryo cells as well as induction of similar particles in mouse embryo cell cultures with IUDR or BUDR have been reported. The presence of type C virus particles in cultures of normal rat embryos has not been reported.NB-1, a culture derived from embryos of a New Zealand Black (NB) rat (rats obtained from Mr. Samuel M. Poiley, N.C.I., Bethesda, Md.) and grown in McCoy's 5A medium supplemented with 20% fetal calf serum was passaged weekly. Extracellular virus particles similar to murine leukemia particles appeared in the 22nd subculture. General appearance of cells in passage 23 is shown in Fig. 1. Two budding figures and one immature type C virus particle may be seen in Fig. 2. The virus particles and budding were present in all further passages examined (currently passage 39). Various stages of budding are shown in Figs. 3a,b,c,d. Appearance of a mature virus particle is shown in Fig. 4.

Use of F9 teratocarcinoma STEM cells to study cell-matrix interactions in the early mouse embryo

1992 ◽  
Vol 50 (1) ◽  
pp. 602-603
Author(s):  
Marc Lenburg ◽  
Rulang Jiang ◽  
Lengya Cheng ◽  
Laura Grabel
Keyword(s):  
Mouse Embryo ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Cell Types ◽  
Embryoid Bodies ◽  
F9 Cells ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions ◽  
F9 Teratocarcinoma

We are interested in defining the cell-cell and cell-matrix interactions that help direct the differentiation of extraembryonic endoderm in the peri-implantation mouse embryo. At the blastocyst stage the mouse embryo consists of an outer layer of trophectoderm surrounding the fluid-filled blastocoel cavity and an eccentrically located inner cell mass. On the free surface of the inner cell mass, facing the blastocoel cavity, a layer of primitive endoderm forms. Primitive endoderm then generates two distinct cell types; parietal endoderm (PE) which migrates along the inner surface of the trophectoderm and secretes large amounts of basement membrane components as well as tissue-type plasminogen activator (tPA), and visceral endoderm (VE), a columnar epithelial layer characterized by tight junctions, microvilli, and the synthesis and secretion of α-fetoprotein. As these events occur after implantation, we have turned to the F9 teratocarcinoma system as an in vitro model for examining the differentiation of these cell types. When F9 cells are treated in monolayer with retinoic acid plus cyclic-AMP, they differentiate into PE. In contrast, when F9 cells are treated in suspension with retinoic acid, they form embryoid bodies (EBs) which consist of an outer layer of VE and an inner core of undifferentiated stem cells. In addition, we have established that when VE containing embryoid bodies are plated on a fibronectin coated substrate, PE migrates onto the matrix and this interaction is inhibited by RGDS as well as antibodies directed against the β1 integrin subunit. This transition is accompanied by a significant increase in the level of tPA in the PE cells. Thus, the outgrowth system provides a spatially appropriate model for studying the differentiation and migration of PE from a VE precursor.

Sign in / Sign up

Export Citation Format

Share Document