Two-Dimensional Gel Electrophoresis of DNA Replication Intermediates in Schizosaccharomyces pombe

2017 ◽  
Vol 2018 (3) ◽  
pp. pdb.prot092007 ◽  
Author(s):  
Mar Sánchez ◽  
Francisco Antequera
Keyword(s):  
Dna Replication ◽  
Schizosaccharomyces Pombe ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Replication Intermediates

A computer model for the analysis of DNA replication intermediates by two-dimensional agarose gel electrophoresis

Gene ◽  
1998 ◽  
Vol 217 (1-2) ◽  
pp. 41-49 ◽  
Author(s):  
E. Viguera ◽  
A. Rodrı́guez ◽  
P. Hernández ◽  
D.B. Krimer ◽  
O. Trellez ◽  
...  
Keyword(s):  
Dna Replication ◽  
Computer Model ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Two Dimensional ◽  
Agarose Gel Electrophoresis ◽  
Replication Intermediates

scholarly journals Origin activation and formation of single-strand TG1-3 tails occur sequentially in late S phase on a yeast linear plasmid.

1993 ◽  
Vol 13 (7) ◽  
pp. 4057-4065 ◽  
Author(s):  
R J Wellinger ◽  
A J Wolf ◽  
V A Zakian
Keyword(s):  
Dna Replication ◽  
Gel Electrophoresis ◽  
S Phase ◽  
Single Strand ◽  
Linear Plasmid ◽  
Replication Forks ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Telomere Replication ◽  
Density Transfer

In order to understand the mechanisms leading to the complete duplication of linear eukaryotic chromosomes, the temporal order of the events involved in replication of a 7.5-kb Saccharomyces cerevisiae linear plasmid called YLpFAT10 was determined. Two-dimensional agarose gel electrophoresis was used to map the position of the replication origin and the direction of replication fork movement through the plasmid. Replication began near the center of YLpFAT10 at the site in the 2 microns sequences that corresponds to the 2 microns origin of DNA replication. Replication forks proceeded bidirectionally from the origin to the ends of YLpFAT10. Thus, yeast telomeres do not themselves act as origins of DNA replication. The time of origin utilization on YLpFAT10 and on circular 2 microns DNA in the same cells was determined both by two-dimensional gel electrophoresis and by density transfer experiments. As expected, 2 microns DNA replicated in early S phase. However, replication of YLpFAT10 occurred in late S phase. Thus, the time of activation of the 2 microns origin depended upon its physical context. Density transfer experiments established that the acquisition of telomeric TG1-3 single-strand tails, a predicted intermediate in telomere replication, occurred immediately after the replication forks approached the ends of YLpFAT10. Thus, telomere replication may be the very last step in S phase.

Origin activation and formation of single-strand TG1-3 tails occur sequentially in late S phase on a yeast linear plasmid

1993 ◽  
Vol 13 (7) ◽  
pp. 4057-4065
Author(s):  
R J Wellinger ◽  
A J Wolf ◽  
V A Zakian
Keyword(s):  
Dna Replication ◽  
Gel Electrophoresis ◽  
S Phase ◽  
Single Strand ◽  
Linear Plasmid ◽  
Replication Forks ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Telomere Replication ◽  
Density Transfer

In order to understand the mechanisms leading to the complete duplication of linear eukaryotic chromosomes, the temporal order of the events involved in replication of a 7.5-kb Saccharomyces cerevisiae linear plasmid called YLpFAT10 was determined. Two-dimensional agarose gel electrophoresis was used to map the position of the replication origin and the direction of replication fork movement through the plasmid. Replication began near the center of YLpFAT10 at the site in the 2 microns sequences that corresponds to the 2 microns origin of DNA replication. Replication forks proceeded bidirectionally from the origin to the ends of YLpFAT10. Thus, yeast telomeres do not themselves act as origins of DNA replication. The time of origin utilization on YLpFAT10 and on circular 2 microns DNA in the same cells was determined both by two-dimensional gel electrophoresis and by density transfer experiments. As expected, 2 microns DNA replicated in early S phase. However, replication of YLpFAT10 occurred in late S phase. Thus, the time of activation of the 2 microns origin depended upon its physical context. Density transfer experiments established that the acquisition of telomeric TG1-3 single-strand tails, a predicted intermediate in telomere replication, occurred immediately after the replication forks approached the ends of YLpFAT10. Thus, telomere replication may be the very last step in S phase.

Analysis of soybean chloroplast DNA replication by two-dimensional gel electrophoresis

1993 ◽  
Vol 23 (4) ◽  
pp. 779-792 ◽  
Author(s):  
Lisa A. Hedrick ◽  
Sabine Heinhorst ◽  
Melissa A. White ◽  
Gordon C. Cannon
Keyword(s):  
Dna Replication ◽  
Chloroplast Dna ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Chloroplast Dna Replication

Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

1985 ◽  
Vol 54 (03) ◽  
pp. 626-629 ◽  
Author(s):  
M Meyer ◽  
F H Herrmann
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Type Ii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Structural Variant ◽  
Glanzmann’S Thrombasthenia ◽  
Crossed Immunoelectrophoresis ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Proteins

SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

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