scholarly journals Time-Lapse Imaging of Cell Death

2016 ◽  
Vol 2016 (3) ◽  
pp. pdb.prot087395 ◽  
Author(s):  
Fredrik Wallberg ◽  
Tencho Tenev ◽  
Pascal Meier
Keyword(s):  
Cell Death ◽  
Time Lapse ◽  
Time Lapse Imaging

scholarly journals Sindbis Virus-Induced Neuronal Death Is both Necrotic and Apoptotic and Is Ameliorated byN-Methyl-d-Aspartate Receptor Antagonists

2001 ◽  
Vol 75 (15) ◽  
pp. 7114-7121 ◽  
Author(s):  
Jennifer L. Nargi-Aizenman ◽  
Diane E. Griffin
Keyword(s):  
Cell Death ◽  
Cortical Neurons ◽  
Sindbis Virus ◽  
Apoptotic Cell ◽  
Time Lapse ◽  
Apoptotic Cell Death ◽  
Time Lapse Imaging ◽  
Alphavirus Infection

ABSTRACT Virus infection of neurons leads to different outcomes ranging from latent and noncytolytic infection to cell death. Viruses kill neurons directly by inducing either apoptosis or necrosis or indirectly as a result of the host immune response. Sindbis virus (SV) is an alphavirus that induces apoptotic cell death both in vitro and in vivo. However, apoptotic changes are not always evident in neurons induced to die by alphavirus infection. Time lapse imaging revealed that SV-infected primary cortical neurons exhibited both apoptotic and necrotic morphological features and that uninfected neurons in the cultures also died. Antagonists of the N-methyl-d-aspartate (NMDA) subtype of glutamate receptors protected neurons from SV-induced death without affecting virus replication or SV-induced apoptotic cell death. These results provide evidence that SV infection activates neurotoxic pathways that result in aberrant NMDA receptor stimulation and damage to infected and uninfected neurons.

scholarly journals Large-Scale Analysis of Cell Death Phenotypic Heterogeneity

2020 ◽  
Author(s):  
Zintis Inde ◽  
Giovanni C. Forcina ◽  
Kyle Denton ◽  
Scott J. Dixon
Keyword(s):  
Cell Death ◽  
Large Scale ◽  
Molecular Mechanisms ◽  
Mapk Pathway ◽  
Time Lapse ◽  
Phenotypic Heterogeneity ◽  
Mitogen Activated Protein Kinase ◽  
Large Scale Analysis ◽  
Mitogen Activated Protein ◽  
Time Lapse Imaging

SUMMARYIndividual cancer cells within a population can exhibit substantial phenotypic heterogeneity such that exposure to a lethal agent will kill only a fraction of cells at a given time. Whether common molecular mechanisms govern this fractional killing in response to different lethal stimuli is poorly understood. In part, this is because it is difficult to compare fractional killing between conditions using existing approaches. Here, we show that fractional killing can be quantified and compared for hundreds of populations in parallel using high-throughput time-lapse imaging. We find that fractional killing is highly variable between lethal agents and between cell lines. At the molecular level, we find that the antiapoptotic protein MCL1 is an important determinant of fractional killing in response to mitogen activated protein kinase (MAPK) pathway inhibitors but not other lethal stimuli. These studies lay the foundation for the large-scale, quantitative analysis of cell death phenotypic heterogeneity.

scholarly journals Time-lapse imaging of cell death in cell culture and whole living organisms using turn-on deep-red fluorescent probes

2018 ◽  
Vol 6 (30) ◽  
pp. 4963-4971 ◽  
Author(s):  
Tia S. Jarvis ◽  
Felicia M. Roland ◽  
Kyle M. Dubiak ◽  
Paul W. Huber ◽  
Bradley D. Smith
Keyword(s):  
Cell Culture ◽  
Cell Death ◽  
Fluorescence Imaging ◽  
Fluorescent Probes ◽  
Time Lapse ◽  
Frog Embryo ◽  
Non Invasive ◽  
Turn On ◽  
Living Organisms ◽  
Time Lapse Imaging

Targeted solvatochromic probe enables non-invasive, time-lapse fluorescence imaging of cell death in cell culture and living frog embryo.

Cleavage of Human Embryos: Options and Diversity

Acta Naturae ◽  
2016 ◽  
Vol 8 (3) ◽  
pp. 88-96
Author(s):  
Yu. K. Doronin ◽  
I. V. Senechkin ◽  
L. V. Hilkevich ◽  
M. A. Kurcer
Keyword(s):  
Time Lapse ◽  
Reproductive Technologies ◽  
Human Embryos ◽  
Imaging Data ◽  
Noninvasive Methods ◽  
Topographic Features ◽  
Time Parameters ◽  
Embryo Cleavage ◽  
Time Lapse Imaging ◽  
Developmental Dynamics

In order to estimate the diversity of embryo cleavage relatives to embryo progress (blastocyst formation), time-lapse imaging data of preimplantation human embryo development were used. This retrospective study is focused on the topographic features and time parameters of the cleavages, with particular emphasis on the lengths of cleavage cycles and the genealogy of blastomeres in 2- to 8-cell human embryos. We have found that all 4-cell human embryos have four developmental variants that are based on the sequence of appearance and orientation of cleavage planes during embryo cleavage from 2 to 4 blastomeres. Each variant of cleavage shows a strong correlation with further developmental dynamics of the embryos (different cleavage cycle characteristics as well as lengths of blastomere cycles). An analysis of the sequence of human blastomere divisions allowed us to postulate that the effects of zygotic determinants are eliminated as a result of cleavage, and that, thereafter, blastomeres acquire the ability of own syntheses, regulation, polarization, formation of functional contacts, and, finally, of specific differentiation. This data on the early development of human embryos obtained using noninvasive methods complements and extend our understanding of the embryogenesis of eutherian mammals and may be applied in the practice of reproductive technologies.

scholarly journals Evaluating the utility of time-lapse imaging in the estimation of post-mortem interval: An Australian case study

2019 ◽  
Vol 1 ◽  
pp. 204-210 ◽  
Author(s):  
Alyson Wilson ◽  
Stanley Serafin ◽  
Dilan Seckiner ◽  
Rachel Berry ◽  
Xanthé Mallett
Keyword(s):  
Time Lapse ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Australian Case ◽  
Time Lapse Imaging

scholarly journals Time-lapse imaging of CO2 migration within near-surface sediments during a controlled sub-seabed release experiment

2021 ◽  
Vol 109 ◽  
pp. 103363
Author(s):  
Ben Roche ◽  
Jonathan M. Bull ◽  
Hector Marin-Moreno ◽  
Timothy G. Leighton ◽  
Ismael H. Falcon-Suarez ◽  
...  
Keyword(s):  
Surface Sediments ◽  
Time Lapse ◽  
Near Surface ◽  
Release Experiment ◽  
Time Lapse Imaging

scholarly journals Antagonizing the spindle assembly checkpoint silencing enhances paclitaxel and Navitoclax-mediated apoptosis with distinct mechanistic

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ana C. Henriques ◽  
Patrícia M. A. Silva ◽  
Bruno Sarmento ◽  
Hassan Bousbaa
Keyword(s):  
Cell Death ◽  
Cancer Cells ◽  
Cell Fate ◽  
Spindle Assembly Checkpoint ◽  
Spindle Assembly ◽  
Time Lapse ◽  
Antimitotic Drugs ◽  
Mitotic Slippage ◽  
Mitotic Death ◽  
Chronic Activation

AbstractAntimitotic drugs arrest cells in mitosis through chronic activation of the spindle assembly checkpoint (SAC), leading to cell death. However, drug-treated cancer cells can escape death by undergoing mitotic slippage, due to premature mitotic exit. Therefore, overcoming slippage issue is a promising chemotherapeutic strategy to improve the effectiveness of antimitotics. Here, we antagonized SAC silencing by knocking down the MAD2-binding protein p31comet, to delay mitotic slippage, and tracked cancer cells treated with the antimitotic drug paclitaxel, over 3 days live-cell time-lapse analysis. We found that in the absence of p31comet, the duration of mitotic block was increased in cells challenged with nanomolar concentrations of paclitaxel, leading to an additive effects in terms of cell death which was predominantly anticipated during the first mitosis. As accumulation of an apoptotic signal was suggested to prevent mitotic slippage, when we challenged p31comet-depleted mitotic-arrested cells with the apoptosis potentiator Navitoclax (previously called ABT-263), cell fate was shifted to accelerated post-mitotic death. We conclude that inhibition of SAC silencing is critical for enhancing the lethality of antimitotic drugs as well as that of therapeutic apoptosis-inducing small molecules, with distinct mechanisms. The study highlights the potential of p31comet as a target for antimitotic therapies.

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