Labeling Second-Order Sensory Neurons in the Posterior Lateral-Line System of Zebrafish

2013 ◽  
Vol 2013 (12) ◽  
pp. pdb.prot079475-pdb.prot079475
Author(s):  
K. Schuster ◽  
A. Ghysen
Keyword(s):  
Sensory Neurons ◽  
Lateral Line ◽  
Second Order ◽  
Lateral Line System ◽  
Line System ◽  
Posterior Lateral Line

scholarly journals Expression of zebrafish Brn1.2 (Pou3f2) and two Brn-3a (Pou4f1) POU genes in brain and sensory structures

2021 ◽  
Author(s):  
Satish Kitambi ◽  
Gayathri Chandrasekar
Keyword(s):  
Sensory Neurons ◽  
Lateral Line ◽  
In Situ Hybridisation ◽  
Neuronal Cell ◽  
Lateral Line System ◽  
Class Iii ◽  
Line System ◽  
Posterior Lateral Line ◽  
Neuronal Cell Differentiation ◽  
Sensory Structures

POU genes are characterized by a conserved POU DNA-binding domain, and are divided into six subclasses. Class III and IV POU genes are predominantly expressed in the developing nervous system. POU class III genes are critical for several neuronal cell differentiation and class IV POU genes serve important functions in the differentiation and survival of sensory neurons. In this study, we attempted to identify POU genes in the zebrafish and pufferfish genomes by using existing bioinformatics tools. We analysed the expression of zebrafish brn1.2 and brn3a genes (brn3a1 and brn3a2)) using whole-mount in situ hybridisation. Similarly to the mammalian orthologue, zebrafish brn1.2 was widely expressed in the forebrain, midbrain and hindbrain. During the late stages of embryogenesis, brn1.2 expressing cells were located in the preoptic area and in the auditory vesicles. Expression of both zebrafish brn3a genes was detected in trigeminal ganglia, cranial sensory ganglia, sensory neurons along the dorsal spinal cord, in the anterior and posterior lateral line placodes (ALL and PLL), retinal ganglion cell layer, optic tectum and in small cell clusters in the forebrain and hindbrain. Similar to mammalian Brn3a, zebrafish brn3a genes were detected in the retina and sensory structures. However, different domains of expression were also observed, namely in spinal sensory neurons, and lateral line system.

scholarly journals Development of the posterior lateral line system in Thunnus thynnus, the atlantic blue-fin tuna, and in its close relative Sarda sarda

2010 ◽  
Vol 54 (8-9) ◽  
pp. 1317-1322 ◽  
Author(s):  
Alain Ghysen ◽  
Kevin Schuster ◽  
Denis Coves ◽  
Fernando de la Gandara ◽  
Nikos Papandroulakis ◽  
...  
Keyword(s):  
Lateral Line ◽  
Close Relative ◽  
Lateral Line System ◽  
Thunnus Thynnus ◽  
Line System ◽  
Posterior Lateral Line ◽  
Sarda Sarda

Electroreceptors and Direction Specific Arrangement in the Lateral Line System of Salamanders?

1981 ◽  
Vol 36 (5-6) ◽  
pp. 493-496 ◽  
Author(s):  
Bernd Fritzsch
Keyword(s):  
Lateral Line ◽  
Fiber Bundles ◽  
Sensory Cells ◽  
Lateral Line System ◽  
Line System ◽  
Lateral Line Organ ◽  
Posterior Lateral Line ◽  
Afferent Fibers ◽  
Lateral Line Afferents ◽  
Line Organ

Abstract The arrangement of the lateral line afferents of salamanders as revealed by transganglionic staining with horse­ radish peroxidase is described. Each lateral line organ is supplied by two fibers only. In the medulla these two afferent fibers run in separate fiber bundles. It is suggested, that only those fibers contacting lateral line sensory cells with the same polarity form together one bundle. Bundles formed by anterior or posterior lateral line afferents are also clearly separated. Beside the lateral line organs smaller pit organs are described. These organs are supplied by one afferent only which reveals an arrangement in the medulla different from that of the lateral line afferents. Based on anatomical facts, these small pit organs are considered to be electroreceptors. Centrifugally projecting neurons, most probably efferents, are described in the medulla.

scholarly journals Cell Proliferation and Collective Cell Migration During Zebrafish Lateral Line System Development Are Regulated by Ncam/Fgf-Receptor Interactions

2021 ◽  
Vol 8 ◽  
Author(s):  
Ramona Dries ◽  
Annemarie Lange ◽  
Sebastian Heiny ◽  
Katja I. Berghaus ◽  
Martin Bastmeyer ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Lateral Line ◽  
System Development ◽  
Lateral Line System ◽  
Collective Cell Migration ◽  
Fgf Receptor ◽  
Line System ◽  
Posterior Lateral Line ◽  
Neural Cell Adhesion ◽  
Reduce Cell Proliferation

The posterior lateral line system (pLLS) of aquatic animals comprises small clustered mechanosensory organs along the side of the animal. They develop from proneuromasts, which are deposited from a migratory primordium on its way to the tip of the tail. We here show, that the Neural Cell Adhesion Molecule Ncam1b is an integral part of the pathways initiating and regulating the development of the pLLS in zebrafish. We find that morpholino-knockdowns of ncam1b (i) reduce cell proliferation within the primordium, (ii) reduce the expression of Fgf target gene erm, (iii) severely affect proneuromast formation, and (iv) affect primordium migration. Ncam1b directly interacts with Fgf receptor Fgfr1a, and a knockdown of fgfr1a causes similar phenotypic changes as observed in ncam1b-morphants. We conclude that Ncam1b is involved in activating proliferation by triggering the expression of erm. In addition, we demonstrate that Ncam1b is required for the expression of chemokine receptor Cxcr7b, which is crucial for directed primordial migration. Finally, we show that the knockdown of ncam1b destabilizes proneuromasts, suggesting a further function of Ncam1b in strengthening the cohesion of proneuromast cells.

scholarly journals Origin and Early Development of the Posterior Lateral Line System of Zebrafish

2010 ◽  
Vol 30 (24) ◽  
pp. 8234-8244 ◽  
Author(s):  
A. F. Sarrazin ◽  
V. A. Nunez ◽  
D. Sapede ◽  
V. Tassin ◽  
C. Dambly-Chaudiere ◽  
...  
Keyword(s):  
Early Development ◽  
Lateral Line ◽  
Lateral Line System ◽  
Line System ◽  
Posterior Lateral Line

scholarly journals Mutations affecting the formation of posterior lateral line system in Medaka, Oryzias latipes

2004 ◽  
Vol 121 (7-8) ◽  
pp. 729-738 ◽  
Author(s):  
Akihito Yasuoka ◽  
Yukihiro Hirose ◽  
Hiroki Yoda ◽  
Yoshiko Aihara ◽  
Hiroshi Suwa ◽  
...  
Keyword(s):  
Lateral Line ◽  
Oryzias Latipes ◽  
Lateral Line System ◽  
Line System ◽  
Posterior Lateral Line

scholarly journals Physiology of afferent neurons in larval zebrafish provides a functional framework for lateral line somatotopy

2012 ◽  
Vol 107 (10) ◽  
pp. 2615-2623 ◽  
Author(s):  
James C. Liao ◽  
Melanie Haehnel
Keyword(s):  
Firing Rate ◽  
Lateral Line ◽  
Input Resistance ◽  
Lateral Line System ◽  
Spontaneous Firing ◽  
Afferent Neurons ◽  
Line System ◽  
Larval Zebrafish ◽  
Posterior Lateral Line ◽  
Spontaneous Firing Rate

Fishes rely on the neuromasts of their lateral line system to detect water flow during behaviors such as predator avoidance and prey localization. Although the pattern of neuromast development has been a topic of detailed research, we still do not understand the functional consequences of its organization. Previous work has demonstrated somatotopy in the posterior lateral line, whereby afferent neurons that contact more caudal neuromasts project more dorsally in the hindbrain than those that contact more rostral neuromasts (Gompel N, Dambly-Chaudiere C, Ghysen A. Development 128: 387–393, 2001). We performed patch-clamp recordings of afferent neurons that contact neuromasts in the posterior lateral line of anesthetized, transgenic larval zebrafish ( Danio rerio) to show that larger cells are born earlier, have a lower input resistance, a lower spontaneous firing rate, and tend to contact multiple neuromasts located closer to the tail than smaller neurons, which are born later, have a higher input resistance, a higher spontaneous firing rate, and tend to contact single neuromasts. We suggest that early-born neurons are poised to detect large stimuli during the initial stages of development. Later-born neurons are more easily driven to fire and thus likely to be more sensitive to local, weaker flows. Afferent projections onto identified glutamatergic regions in the hindbrain lead us to hypothesize a novel mechanism for lateral line somatotopy. We show that afferent fibers associated with tail neuromasts respond to stronger stimuli and are wired to dorsal hindbrain regions associated with Mauthner-mediated escape responses and fast, avoidance swimming. The ability to process flow stimuli by circumventing higher-order brain centers would ease the task of processing where speed is of critical importance. Our work lays the groundwork to understand how the lateral line translates flow stimuli into appropriate behaviors at the single cell level.

scholarly journals Diversity of lateral line patterns and neuromast numbers in the genus Oryzias

2021 ◽  
Vol 224 (24) ◽  
Author(s):  
Ali Seleit ◽  
Satoshi Ansai ◽  
Kazunori Yamahira ◽  
Kawilarang W. A. Masengi ◽  
Kiyoshi Naruse ◽  
...  
Keyword(s):  
Lateral Line ◽  
Strong Dependence ◽  
Lateral Line System ◽  
Cluster Number ◽  
Line System ◽  
Posterior Lateral Line ◽  
Polygenic Control ◽  
Lateral Lines ◽  
Rapid Diversification ◽  
Line Patterns

ABSTRACT A remarkable diversity of lateral line patterns exists in adult teleost fishes, the basis of which is largely unknown. By analysing the lateral line patterns and organ numbers in 29 Oryzias species and strains we report a rapid diversification of the lateral line system within this genus. We show a strong dependence of lateral line elaboration (number of neuromasts per cluster, number of parallel lateral lines) on adult species body size irrespective of phylogenetic relationships. In addition, we report that the degree of elaboration of the anterior lateral line, posterior lateral line and caudal neuromast clusters is tightly linked within species, arguing for a globally coordinated mechanism controlling lateral line organ numbers and patterns. We provide evidence for a polygenic control over neuromast numbers and positioning in the genus Oryzias. Our data also indicate that the diversity in lateral lines can arise as a result of differences in patterning both during embryonic development and post-embryonically, where simpler embryonic patterns generate less complex adult patterns and organ numbers, arguing for a linkage between the two processes.

scholarly journals Expression of two uncharacterized protein coding genes in zebrafish lateral line system

2021 ◽  
Author(s):  
Sana Fatma ◽  
Ravindra Kumar ◽  
Anshuman Dixit ◽  
Rajeeb K. Swain
Keyword(s):  
Lateral Line ◽  
Molecular Level ◽  
System Development ◽  
Signalling Pathways ◽  
Lateral Line System ◽  
Uncharacterized Protein ◽  
Protein Coding ◽  
Line System ◽  
Posterior Lateral Line ◽  
Delayed Initiation

The lateral line system is a mechanosensory organ of fish and amphibians that detects changes in water flow and is formed by the coordinated action of many signalling pathways. These signalling pathways can easily be targeted in zebrafish using pharmacological inhibitors to decipher their role in lateral line system development at cellular and molecular level. We have identified two uncharacterized proteins, whose mRNA are expressed in the lateral line system of zebrafish. One of these proteins, uncharacterized protein LOC564095 precursor is conserved across vertebrates and its mRNA is expressed in posterior lateral line primordium (pLLP). The other uncharacterized protein LOC100536887 is present only in the teleost fishes and its mRNA is expressed in neuromasts. We show that inhibition of retinoic acid (RA) signalling reduces the expression of both of these uncharacterized genes. It is reported that inhibition of RA signalling during gastrulation starting at 7 hours post fertilization (hpf) abrogates pLLP formation and inhibition of RA signalling at 10 hpf delays the initiation of pLLP migration. Here, we show that inhibition of RA signalling before and during segmentation (9-16 hpf) results in delayed initiation and reduced speed of pLLP migration as well as inhibition of posterior neuromasts formation.

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