scholarly journals Preparation of Urogenital Sinus Mesenchymal Cells for Prostate Tissue Recombination Models

2015 ◽  
Vol 2015 (11) ◽  
pp. pdb.prot078055 ◽  
Author(s):  
Yang Zong ◽  
Andrew S. Goldstein ◽  
Owen N. Witte
Keyword(s):  
Mesenchymal Cells ◽  
Prostate Tissue ◽  
Urogenital Sinus ◽  
Tissue Recombination

scholarly journals Estrogen receptor 1 expression and methylation of Esr1 promoter in mouse fetal prostate mesenchymal cells induced by gestational exposure to bisphenol A or ethinylestradiol

2019 ◽  
Vol 5 (3) ◽  
Author(s):  
Ramji K Bhandari ◽  
Julia A Taylor ◽  
Jennifer Sommerfeld-Sager ◽  
Donald E Tillitt ◽  
William A Ricke ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Bisphenol A ◽  
Oral Contraceptives ◽  
Primary Cultures ◽  
Prostate Gland ◽  
Mesenchymal Cells ◽  
Urogenital Sinus ◽  
Environmental Estrogen ◽  
Mouse Prostate

Abstract Fetal/neonatal environmental estrogen exposures alter developmental programing of the prostate gland causing onset of diseases later in life. We have previously shown in vitro that exposures to 17β-estradiol (E2) and the endocrine disrupting chemical bisphenol A, at concentrations relevant to human exposure, cause an elevation of estrogen receptor α (Esr1) mRNA in primary cultures of fetal mouse prostate mesenchymal cells; a similar result was observed in the fetal rat urogenital sinus. Effects of these chemicals on prostate mesenchyme in vivo are not well understood. Here we show effects in mice of fetal exposure to the estrogenic drug in mixed oral contraceptives, 17α-ethinylestradiol (EE2), at a concentration of EE2 encountered by human embryos/fetuses whose mothers become pregnant while on EE2-containing oral contraceptives, or bisphenol A at a concentration relevant to exposures observed in human fetuses in vivo. Expression of Esr1 was elevated by bisphenol A or EE2 exposures, which decreased the global expression of DNA methyltransferase 3A (Dnmt3a), while methylation of Esr1 promoter was significantly increased. These results show that exposures to the environmental estrogen bisphenol A and drug EE2 cause transcriptional and epigenetic alterations to expression of estrogen receptors in developing prostate mesenchyme in vivo.

scholarly journals High-throughput propagation of human prostate tissue from induced-pluripotent stem cells

2019 ◽  
Author(s):  
AC Hepburn ◽  
EL Curry ◽  
M Moad ◽  
RE Steele ◽  
OE Franco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Neuroendocrine Cells ◽  
Prostate Tissue ◽  
Urogenital Sinus ◽  
Human Prostate ◽  
Human Prostate Tissue ◽  
Induced Pluripotent

AbstractPrimary culture of human prostate organoids is slow, inefficient and laborious. To overcome this, we demonstrate a new high-throughput model where rapidly proliferating and easily handled induced pluripotent stem cells, for the first time, enable generation of human prostate tissue in vivo and in vitro. Using a co-culture technique with urogenital sinus mesenchyme, we recapitulated the in situ prostate histology, including the stromal compartment and the full spectrum of epithelial differentiation. This approach overcomes major limitations in primary cultures of human prostate stem, luminal and neuroendocrine cells, as well as the stromal microenvironment. These models provide new opportunities to study prostate development, homeostasis and disease.

Interactions between adult human prostatic epithelium and rat urogenital sinus mesenchyme in a tissue recombination model

1998 ◽  
Vol 63 (3) ◽  
pp. 131-140 ◽  
Author(s):  
Simon W. Hayward ◽  
Peter C. Haughney ◽  
Mark A. Rosen ◽  
Karin M. Greulich ◽  
Heinz-Ulrich G. Weier ◽  
...  
Keyword(s):  
Urogenital Sinus ◽  
Adult Human ◽  
Recombination Model ◽  
Prostatic Epithelium ◽  
Tissue Recombination

Induction of prostatic morphology and secretion in urothelium by seminal vesicle mesenchyme

Development ◽  
1995 ◽  
Vol 121 (7) ◽  
pp. 2199-2207 ◽  
Author(s):  
A.A. Donjacour ◽  
G.R. Cunha
Keyword(s):  
Seminal Vesicle ◽  
Reproductive Tract ◽  
Urogenital Sinus ◽  
Secretory Proteins ◽  
Ductus Deferens ◽  
Male Reproductive Tract ◽  
Mesodermal Origin ◽  
Tissue Recombination ◽  
Endodermal Origin ◽  
Recombination Experiments

Mesenchymal-epithelial interactions are essential for the development of the male reproductive tract. Tissue recombination experiments have been used to define the characteristics of these interactions. When mesenchyme, embryonic connective tissue, is recombined with epithelium from another organ an instructive induction may occur in which the developmental fate of the epithelium is altered. Instructive inductions are most common when the epithelium that is removed from the mesenchyme and the epithelium that is recombined with the mesenchyme are from the same germ layer. All of the mesenchyme of the male reproductive tract is of mesodermal origin. The epithelia of these organs are derived from either the mesodermal Wolffian duct epithelium or the endodermal urogenital sinus epithelium. Urogenital sinus mesenchyme can instructively induce bladder and urethral epithelium to form prostate (Donjacour, A. A. and Cunha, G. R. (1993) Endocrinol. 132, 2342–2350) and seminal vesicle mesenchyme can instructively induce epithelium from the ductus deferens and ureter (Cunha, G. R., Young, P., Higgins, S. J. and Cooke, P. S. (1991) Development 111, 145–158) to form seminal vesicle. To see whether inductive interactions could occur across germ layers in this system, seminal vesicle mesenchyme, normally associated with a mesodermal epithelium, was recombined with epithelium from neonatal or adult bladder or urethra, which are of endodermal origin. The resulting tissue recombinants were analyzed histologically and by immunocytochemistry and western blotting with antibodies to prostatic and seminal vesicle secretory proteins. Full prostatic differentiation was observed in tissue recombinants made with seminal vesicle mesenchyme plus either adult or neonatal bladder or urethral epithelium.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Purification of a Novel Protein (ps20) from Urogenital Sinus Mesenchymal Cells with Growth Inhibitory Propertiesin Vitro

1995 ◽  
Vol 270 (37) ◽  
pp. 22058-22065 ◽  
Author(s):  
David R. Rowley ◽  
Truong D. Dang ◽  
Melinda Larsen ◽  
Michael J. Gerdes ◽  
Lauren McBride ◽  
...  
Keyword(s):  
Mesenchymal Cells ◽  
Urogenital Sinus ◽  
Growth Inhibitory ◽  
Novel Protein
Sign in / Sign up

Export Citation Format

Share Document