PiggyBac Transposon-Mediated Cellular Transgenesis in Mammalian Forebrain by In Utero Electroporation

2014 ◽  
Vol 2014 (7) ◽  
pp. pdb.prot073650-pdb.prot073650 ◽  
Author(s):  
F. Chen ◽  
B. J. Maher ◽  
J. J. LoTurco
Keyword(s):  
In Utero ◽  
In Utero Electroporation ◽  
Piggybac Transposon

Application of in utero electroporation and live imaging in the analyses of neuronal migration during mouse brain development

2012 ◽  
Vol 45 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Yoshiaki V. Nishimura ◽  
Tomoyasu Shinoda ◽  
Yutaka Inaguma ◽  
Hidenori Ito ◽  
Koh-ichi Nagata
Keyword(s):  
Brain Development ◽  
Mouse Brain ◽  
Neuronal Migration ◽  
In Utero ◽  
Live Imaging ◽  
In Utero Electroporation ◽  
Mouse Brain Development

scholarly journals The hominoid-specific gene TBC1D3 promotes generation of basal neural progenitors and induces cortical folding in mice

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Xiang-Chun Ju ◽  
Qiong-Qiong Hou ◽  
Ai-Li Sheng ◽  
Kong-Yan Wu ◽  
Yang Zhou ◽  
...  
Keyword(s):  
Radial Glia ◽  
Brain Slices ◽  
In Utero ◽  
Specific Gene ◽  
Segmental Duplications ◽  
Cortical Folding ◽  
In Utero Electroporation ◽  
Cellular Mechanisms ◽  
Transgenic Expression ◽  
Cortical Regions

Cortical expansion and folding are often linked to the evolution of higher intelligence, but molecular and cellular mechanisms underlying cortical folding remain poorly understood. The hominoid-specific gene TBC1D3 undergoes segmental duplications during hominoid evolution, but its role in brain development has not been explored. Here, we found that expression of TBC1D3 in ventricular cortical progenitors of mice via in utero electroporation caused delamination of ventricular radial glia cells (vRGs) and promoted generation of self-renewing basal progenitors with typical morphology of outer radial glia (oRG), which are most abundant in primates. Furthermore, down-regulation of TBC1D3 in cultured human brain slices decreased generation of oRGs. Interestingly, localized oRG proliferation resulting from either in utero electroporation or transgenic expression of TBC1D3, was often found to underlie cortical regions exhibiting folding. Thus, we have identified a hominoid gene that is required for oRG generation in regulating the cortical expansion and folding.

scholarly journals Role of Nrp1 in controlling cortical interhemispheric circuits

2021 ◽  
Author(s):  
Fernando Martín Fernández ◽  
Carlos Garcia Briz ◽  
Marta Nieto
Keyword(s):  
Postnatal Growth ◽  
In Utero ◽  
In Utero Electroporation ◽  
Knock Down ◽  
Cortical Areas ◽  
Neuropilin 1

Callosal projections establish topographically organized maps between cortical areas. Neuropilin-1 (Nrp1) cortical gradient induces an early segregation of developing callosal axons. We investigated later roles of Nrp1 on the development of callosal projections from layer (L) 2/3 of the primary (S1) and secondary (S2) somatosensory (SS) areas, which express higher and lower levels of Nrp1, respectively. We used in utero electroporation to knock down or overexpress Nrp1 combined with retrograde tracers, to map connections at postnatal day 16 and 30. High levels of Nrp1 blocked contralateral S2 innervation while promoted the late postnatal growth of homotopic S1L2/3 and heterotopic S2L2/3 branches into S1. Conversely, knocking down Nrp1 increased the growth of heterotopic S1L2/3 projections into S2, and the overall refinement of S2L2/3 branches, thereby diminishing the number of P30 S2L2/3 callosally projecting neurons. Thus, the Nrp1 gradient determines homotopic SSL2/3 callosal connectivity by regulating late postnatal branching and refinement in a topographic manner.

scholarly journals Gene delivery in mouse auditory brainstem and hindbrain using in utero electroporation

2014 ◽  
Vol 7 (1) ◽  
Author(s):  
Laurence S David ◽  
Jamila Aitoubah ◽  
Lee Stephen Lesperance ◽  
Lu-Yang Wang
Keyword(s):  
Gene Delivery ◽  
Auditory Brainstem ◽  
In Utero ◽  
In Utero Electroporation
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