Fiber-Optic Calcium Monitoring of Dendritic Activity In Vivo

2012 ◽  
Vol 2012 (2) ◽  
pp. pdb.prot067835-pdb.prot067835 ◽  
Author(s):  
M. Murayama ◽  
M. Larkum
Keyword(s):  
Fiber Optic

scholarly journals Fiber-Optic Time-Resolved Fluorescence Sensor for in Vitro Serotonin Determination

1993 ◽  
Vol 47 (5) ◽  
pp. 590-597 ◽  
Author(s):  
Stephane Mottin ◽  
Canh Tran-Minh ◽  
Pierre Laporte ◽  
Raymond Cespuglio ◽  
Michel Jouvet
Keyword(s):  
Chemical Sensor ◽  
Fiber Optic ◽  
Fiber Optic Sensor ◽  
Excitation Wavelength ◽  
Nitrogen Laser ◽  
Time Resolved ◽  
Temporal Effects ◽  
Starting Point

At pH 7 and with the excitation at wavelengths above 315 nm, previously unreported fluorescence of 5-HT (5-hydroxytryptamine) is observed. Two fluorescence bands were observed for 5-HT; the first emits at around 390 nm with an associated lifetime near 1 ns, and the other (well known) emits at 340 nm with an associated lifetime of 2.7 ns. With both static and time-resolved fluorescences, the spectral and temporal effects of the excitation wavelength were studied between 285 and 340 nm. With these basic spectroscopic properties as a starting point, a fiber-optic chemical sensor (FOCS) was developed in order to measure 5-HT with a single-fiber configuration, nitrogen laser excitation, and fast digitizing techniques. Temporal effects including fluorescence of the optical fiber were studied and compared with measurements both directly in cuvette and through the fiber-optic sensor. Less than thirty seconds are required for each measurement. A detection limit of 5-HT is reached in the range of 5 μM. Our system, with an improved sensitivity, could therefore be a possible and convenient “tool” for in vivo determination of 5-HT.

scholarly journals Real-time In vivo Diagnosis of Nasopharyngeal Carcinoma Using Rapid Fiber-Optic Raman Spectroscopy

Theranostics ◽  
2017 ◽  
Vol 7 (14) ◽  
pp. 3517-3526 ◽  
Author(s):  
Kan Lin ◽  
Wei Zheng ◽  
Chwee Ming Lim ◽  
Zhiwei Huang
Keyword(s):  
Raman Spectroscopy ◽  
Nasopharyngeal Carcinoma ◽  
Real Time ◽  
Fiber Optic ◽  
In Vivo Diagnosis

Development of fiber optic fluorescence oxygen sensor in both in vitro and in vivo systems

2008 ◽  
Vol 161 (2) ◽  
pp. 160-166 ◽  
Author(s):  
Jinjun Jiang ◽  
Lei Gao ◽  
Wei Zhong ◽  
Shen Meng ◽  
Ben Yong ◽  
...  
Keyword(s):  
Oxygen Sensor ◽  
Fiber Optic

A fiber optic approach for in vivo minimally invasive study of tissue optical properties

2004 ◽  
Author(s):  
Ilko K. Ilev ◽  
Ronald W. Waynant ◽  
Kimberly R. Byrnes ◽  
Juanita Anders
Keyword(s):  
Optical Properties ◽  
Minimally Invasive ◽  
Fiber Optic ◽  
Tissue Optical Properties

In vivo cellular imaging pinpoints the role of reactive oxygen species in the early steps of adult hematopoietic reconstitution

Blood ◽  
2010 ◽  
Vol 115 (3) ◽  
pp. 443-452 ◽  
Author(s):  
Daniel Lewandowski ◽  
Vilma Barroca ◽  
Frédéric Ducongé ◽  
Jan Bayer ◽  
Jeanne Tran Van Nhieu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Femoral Head ◽  
Fiber Optic ◽  
Imaging System ◽  
Hematopoietic Cells ◽  
Oxygen Species ◽  
Hematopoietic Reconstitution

Abstract Few techniques are available to characterize in vivo the early cellular dynamics of long-term reconstitution of hematopoiesis after transplantation of hematopoietic stem cells (HSCs) after lethal irradiation. Using a fiber-optic imaging system, we track the early steps of in vivo recruitment and proliferation of Lin−Sca-1+c-Kit+CD34− (LSKCD34−) HSCs highly enriched in HSCs and transplanted into lethally irradiated mice. Recruitment of the transplanted LSKCD34− hematopoietic cells first occurs in the femoral head and is continuous during 24 hours. Quantification of the fluorescence emitted by the transplanted hematopoietic cells shows that proliferation of LSKCD34− hematopoietic cells in the femoral head was potent 3 days after transplantation. Using a development of this fiber-optic imaging system, we show that the transplanted LSKCD34− hematopoietic cells are associated with vascularized structures as early as 5 hours after transplantation. This early association is dependent on reactive oxygen species (ROS) partly through the regulation of vascular cell adhesion molecule-1 expression on endothelial cells and is followed by a ROS-dependent proliferation of LSKCD34− hematopoietic cells. This new in vivo imaging technique permits the observation of the early steps of hematopoietic reconstitution by HSCs in long bones and shows a new role of ROS in the recruitment of HSCs by bone marrow endothelial cells.

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