Digital Scanned Laser Light-Sheet Fluorescence Microscopy (DSLM) of Zebrafish and Drosophila Embryonic Development

2011 ◽  
Vol 2011 (10) ◽  
pp. pdb.prot065839-pdb.prot065839 ◽  
Author(s):  
P. J. Keller ◽  
A. D. Schmidt ◽  
J. Wittbrodt ◽  
E. H. K. Stelzer
Keyword(s):  
Embryonic Development ◽  
Fluorescence Microscopy ◽  
Laser Light ◽  
Light Sheet ◽  
Laser Light Sheet ◽  
Light Sheet Fluorescence Microscopy

Digital Scanned Laser Light Sheet Fluorescence Microscopy

2010 ◽  
Vol 2010 (5) ◽  
pp. pdb.top78-pdb.top78 ◽  
Author(s):  
P. J. Keller ◽  
E. H. K. Stelzer
Keyword(s):  
Fluorescence Microscopy ◽  
Laser Light ◽  
Light Sheet ◽  
Laser Light Sheet ◽  
Light Sheet Fluorescence Microscopy

scholarly journals Cytotoxic effects and tolerability of gemcitabine and axitinib in a xenograft model for c-myc amplified medulloblastoma

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Stefanie Schwinn ◽  
Zeinab Mokhtari ◽  
Sina Thusek ◽  
Theresa Schneider ◽  
Anna-Leena Sirén ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Tumor Growth ◽  
Intensive Therapy ◽  
Xenograft Model ◽  
Light Sheet ◽  
Tumor Control ◽  
Orthotopic Model ◽  
Group 3 ◽  
Light Sheet Fluorescence Microscopy

AbstractMedulloblastoma is the most common high-grade brain tumor in childhood. Medulloblastomas with c-myc amplification, classified as group 3, are the most aggressive among the four disease subtypes resulting in a 5-year overall survival of just above 50%. Despite current intensive therapy regimens, patients suffering from group 3 medulloblastoma urgently require new therapeutic options. Using a recently established c-myc amplified human medulloblastoma cell line, we performed an in-vitro-drug screen with single and combinatorial drugs that are either already clinically approved or agents in the advanced stage of clinical development. Candidate drugs were identified in vitro and then evaluated in vivo. Tumor growth was closely monitored by BLI. Vessel development was assessed by 3D light-sheet-fluorescence-microscopy. We identified the combination of gemcitabine and axitinib to be highly cytotoxic, requiring only low picomolar concentrations when used in combination. In the orthotopic model, gemcitabine and axitinib showed efficacy in terms of tumor control and survival. In both models, gemcitabine and axitinib were better tolerated than the standard regimen comprising of cisplatin and etoposide phosphate. 3D light-sheet-fluorescence-microscopy of intact tumors revealed thinning and rarefication of tumor vessels, providing one explanation for reduced tumor growth. Thus, the combination of the two drugs gemcitabine and axitinib has favorable effects on preventing tumor progression in an orthotopic group 3 medulloblastoma xenograft model while exhibiting a favorable toxicity profile. The combination merits further exploration as a new approach to treat high-risk group 3 medulloblastoma.

Investigating safe excitation in Light Sheet Fluorescence Microscopy

2021 ◽  
Vol 84 ◽  
pp. 296
Author(s):  
Gideon Oluniran ◽  
James Blackwell ◽  
Emmanuel Reynaud ◽  
Marcin Krasny ◽  
Niall Colgan ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Light Sheet ◽  
Light Sheet Fluorescence Microscopy

scholarly journals Light sheet fluorescence microscopy of optically cleared brains for studying the glymphatic system

2020 ◽  
Vol 40 (10) ◽  
pp. 1975-1986
Author(s):  
Nicholas B Bèchet ◽  
Tekla M Kylkilahti ◽  
Bengt Mattsson ◽  
Martina Petrasova ◽  
Nagesh C Shanbhag ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluid Transport ◽  
Light Sheet ◽  
Brain Parenchyma ◽  
Sleep State ◽  
Glymphatic System ◽  
Light Sheet Microscopy ◽  
Highly Active ◽  
Quantitative Analyses ◽  
Light Sheet Fluorescence Microscopy

Fluid transport in the perivascular space by the glia-lymphatic (glymphatic) system is important for the removal of solutes from the brain parenchyma, including peptides such as amyloid-beta which are implicated in the pathogenesis of Alzheimer’s disease. The glymphatic system is highly active in the sleep state and under the influence of certain of anaesthetics, while it is suppressed in the awake state and by other anaesthetics. Here we investigated whether light sheet fluorescence microscopy of whole optically cleared murine brains was capable of detecting glymphatic differences in sleep- and awake-mimicking anaesthesia, respectively. Using light-sheet imaging of whole brains, we found anaesthetic-dependent cerebrospinal fluid (CSF) influx differences, including reduced tracer influx along tertiary branches of the middle cerebral artery and reduced influx along dorsal and anterior penetrating arterioles, in the awake-mimicking anaesthesia. This study establishes that light sheet microscopy of optically cleared brains is feasible for quantitative analyses and can provide images of the entire glymphatic system in whole brains.

Visualization of Internal Flow Field of Propeller Fan

2017 ◽  
Author(s):  
Takaya Onishi ◽  
H. Sato ◽  
M. Hayakawa ◽  
Y. Kawata
Keyword(s):  
Flow Field ◽  
High Performance ◽  
Laser Light ◽  
Internal Flow ◽  
Light Sheet ◽  
Tip Vortex ◽  
Laser Light Sheet ◽  
Blade Surface ◽  
Similar Tendency ◽  
Flow Phenomena

Propeller fans are required not only to have high performance but also to be extremely quiet. The internal flow field of ventilation propeller fans is even more complicated because they usually have a very peculiar configuration with protruding blades upstream. Thus, many kinds of internal vortices yield which cause noise and their cause and countermeasures are needed to be clarified. The purposes of this paper are to visualize the internal flow of the propeller fan from the static and rotating frame of reference. The internal flow visualization measured from the static frame gives approximately the scale of the tip vortex. The visualization from the rotating coordinate system yields a better understanding of the flow phenomena occurring at the specific blade. The experiment is implemented by using a small camera mounted on the shaft of the fan and rotated it to capture the behavior of the vortices using a laser light sheet to irradiate the blade surface. Hence, the flow field of the specific blade could be understood to some extent. The visualized results are compared with the CFD results and these results show a similar tendency about the generation point and developing process of the tip vortex. In addition, it is found that the noise measurement result is relevant to the effect of tip vortex from the visualization result.

FEATURES

2016 ◽  
Vol 20 (12) ◽  
pp. 12-22
Keyword(s):  
Medical Imaging ◽  
Fluorescence Microscopy ◽  
Fluorescence Spectroscopy ◽  
Medical Images ◽  
Light Sheet ◽  
The Future ◽  
Light Sheet Fluorescence Microscopy

Scanning the Future of Medical Imaging Putting Numbers into Biology: The Combination of Light Sheet Fluorescence Microscopy and Fluorescence Spectroscopy Abyss Processing – Exploring the Deep in Medical Images

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