A region of a PtK2 cell transfected with GFP-keratin 14

2009 ◽  
Vol 2009 (12) ◽  
pp. pdb.mov56-pdb.mov56
Keyword(s):  
Keratin 14 ◽  
Ptk2 Cell

A recurrent keratin 14 mutation in Dowling-Meara epidermolysis bullosa simplex

1999 ◽  
Vol 141 (4) ◽  
pp. 747-748 ◽  
Author(s):  
Sasaki ◽  
Shimizu ◽  
Akiyama ◽  
Hiraoka ◽  
Takizawa ◽  
...  
Keyword(s):  
Epidermolysis Bullosa ◽  
Epidermolysis Bullosa Simplex ◽  
Keratin 14

scholarly journals Short tail with skin lesion phenotype occurs in transgenic mice with keratin-14 promoter-directed expression of mutant CXCR2

2008 ◽  
Vol 84 (2) ◽  
pp. 406-419 ◽  
Author(s):  
Yingchun Yu ◽  
Yingjun Su ◽  
Susan R. Opalenik ◽  
Tammy Sobolik-Delmaire ◽  
Nicole F. Neel ◽  
...  
Keyword(s):  
Skin Lesion ◽  
Transgenic Mice ◽  
Short Tail ◽  
Keratin 14

scholarly journals Individual microtubules viewed by immunofluorescence and electron microscopy in the same PtK2 cell

1978 ◽  
Vol 77 (3) ◽  
pp. R27 ◽  
Author(s):  
M Osborn ◽  
RE Webster ◽  
K Weber
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Light Microscope ◽  
Immunofluorescence Microscopy ◽  
Uranyl Acetate ◽  
Tubulin Antibodies ◽  
Ptk2 Cell ◽  
Cytoplasmic Microtubules ◽  
Triton X ◽  
Microtubular Structures

PtK2 cells were grown on gold grids and treated with Triton X-100 in a microtubule stabilizing buffer. The resulting cytoskeletons were fixed with glutaraldehyde and subjected to the indirect immunofluorescence procedure using monospecific tubulin antibodies. Grids were examined first by fluorescence microscopy, and the display of fluorescent cytoplasmic microtubules was recorded. The grids were then stained with uranyl acetate and the display of fibrous structures recorded by electron microscopy. Thus the display of cytoplasmic microtubular structures in the light microscope and the electron microscope can be compared within the same cytoskeleton. The results show a direct correspondence of the fluorescent fibers in the light microscope with uninterrupted fibers of diameter approximately 550 A in the electron microscope. This is the diameter reported for a single microtubule decorated around its circumference by two layers of antibody molecules. Thus under optimal conditions immunofluorescence microscopy can visualize individual microtubules.

scholarly journals Raf-1 activation disrupts its binding to keratins during cell stress

2004 ◽  
Vol 166 (4) ◽  
pp. 479-485 ◽  
Author(s):  
Nam-On Ku ◽  
Haian Fu ◽  
M. Bishr Omary
Keyword(s):  
Cell Signaling ◽  
Kinase Activity ◽  
Cultured Cells ◽  
Mutational Analysis ◽  
Cell Stress ◽  
Phosphorylation Sites ◽  
Dependent Manner ◽  
Raf Kinase ◽  
Toxin Exposure ◽  
Keratin 14

Keratins 8 and 18 (K8/18) heteropolymers may regulate cell signaling via the known K18 association with 14-3-3 proteins and 14-3-3 association with Raf-1 kinase. We characterized Raf–keratin–14-3-3 associations and show that Raf associates directly with K8, independent of Raf kinase activity or Ras–Raf interaction, and that K18 is a Raf physiologic substrate. Raf activation during oxidative and toxin exposure in cultured cells and animals disrupt keratin–Raf association in a phosphorylation-dependent manner. Mutational analysis showed that 14-3-3 residues that are essential for Raf binding also regulate 14-3-3–keratin association. Similarly, Raf phosphorylation sites that are important for binding to 14-3-3 are also essential for Raf binding to K8/18. Therefore, keratins may modulate some aspects of Raf signaling under basal conditions via sequestration by K8, akin to Raf–14-3-3 binding. Keratin-bound Raf kinase is released upon Raf hyperphosphorylation and activation during oxidative and other stresses.

Stabilizing mutations of KLHL24 ubiquitin ligase cause loss of keratin 14 and human skin fragility

2016 ◽  
Vol 48 (12) ◽  
pp. 1508-1516 ◽  
Author(s):  
Zhimiao Lin ◽  
Shuo Li ◽  
Cheng Feng ◽  
Shang Yang ◽  
Huijun Wang ◽  
...  
Keyword(s):  
Human Skin ◽  
Ubiquitin Ligase ◽  
Keratin 14 ◽  
Skin Fragility
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