Transmission of a TP53 germline mutation from unaffected male carrier associated with pediatric glioblastoma in his child and gestational choriocarcinoma in his female partner

Jennifer A. Cotter; Linda Szymanski; Catherine Karimov; Lara Boghossian; Ashley Margol; Girish Dhall; Benita Tamrazi; G. Isaac Varaprasathan; David M. Parham; Alexander R. Judkins; Jaclyn A. Biegel

doi:10.1101/mcs.a002576

Transmission of a TP53 germline mutation from unaffected male carrier associated with pediatric glioblastoma in his child and gestational choriocarcinoma in his female partner

Molecular Case Studies ◽

10.1101/mcs.a002576 ◽

2018 ◽

Vol 4 (2) ◽

pp. a002576 ◽

Cited By ~ 5

Author(s):

Jennifer A. Cotter ◽

Linda Szymanski ◽

Catherine Karimov ◽

Lara Boghossian ◽

Ashley Margol ◽

...

Keyword(s):

Germline Mutation ◽

Female Partner ◽

Gestational Choriocarcinoma ◽

Pediatric Glioblastoma ◽

Male Carrier

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Ultrastructure of Choriocarcinoma in Vitro

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010006372x ◽

1969 ◽

Vol 27 ◽

pp. 362-363

Author(s):

John C. Garancis ◽

R. A. Pattillo

Keyword(s):

Cell Line ◽

Physiological Function ◽

Cell System ◽

Bewo Cell ◽

Bewo Cells ◽

Gestational Choriocarcinoma ◽

Bewo Cell Line

Growth of cell system (BeWo-cell line) derived from human gestational choriocarcinoma has been established and continuously maintained in-vitro. Furthermore, it is evident from the previous studies that this cell line has retained the physiological function of the placental trophoblasts, namely the synthesis of human chorionic gonadotrophil(HCG).The BeWo cells were relatively small and possessed single nuclei, thus indicating that this cell line consists exclusively of cytotrophoblasts. In some instances cells appeared widely separated and their lateral surfaces were provided with numerous microvilli (Fig.1).

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Comparison of Choriocarcinoma and Normal Placenta

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065663 ◽

1971 ◽

Vol 29 ◽

pp. 324-325

Author(s):

John C. Garancis ◽

Roland A. Pattillo ◽

Robert O. Hussa ◽

Jon V. Straumfjord

Keyword(s):

Cell Lines ◽

Small Cells ◽

Tissue Cultures ◽

Glycogen Depletion ◽

Cell Surfaces ◽

Intercellular Spaces ◽

Concomitant Increase ◽

Gestational Choriocarcinoma ◽

Cytoplasmic Glycogen ◽

Normal Placenta

Two different cell lines (Be-Wo and Jar) of human gestational choriocarcinoma have been maintained in continuous tissue culture for a period of four and two years respectively without losing the ability to elaborate human chorionic gonadotropin (HCG). Tissue cultures, as revealed by electron microscopy, consisted of small cells with single nuclei. In some instances cell surfaces were provided with microvilli but more often the intercellular spaces were narrow and bridged by desmosomes. However, syncytium was not formed. Endoplasmic reticulum (ER) was poorly developed in both cell lines, except in some Be-Wo cells it was prominent. Golgi complex, lysosomes and numerous free ribosomes, as well as excessive cytoplasmic glycogen, were present in all cells (Fig. 1). Glycogen depletion and concomitant increase of ER were observed in many cells following a single dose of 10 ugm/ml of adrenalin added to medium (Fig. 2).

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