scholarly journals Single-strand conformational polymorphism.

1994 ◽  
Vol 4 (3) ◽  
pp. S137-S140 ◽  
Author(s):  
K Fujita ◽  
J Silver
Keyword(s):  
Single Strand ◽  
Single Strand Conformational Polymorphism ◽  
Conformational Polymorphism

Determination of apolipoprotein E genotypes by single-strand conformational polymorphism

1993 ◽  
Vol 39 (10) ◽  
pp. 2121-2124 ◽  
Author(s):  
M Y Tsai ◽  
P Suess ◽  
K Schwichtenberg ◽  
J H Eckfeldt ◽  
J Yuan ◽  
...  
Keyword(s):  
Amino Acid ◽  
Apolipoprotein E ◽  
Single Strand ◽  
Amplification Refractory Mutation System ◽  
Amino Acid Residues ◽  
Single Strand Conformational Polymorphism ◽  
Conformational Polymorphism ◽  
Apo E ◽  
Apolipoprotein E Genotypes ◽  
Mutation System

Abstract We used single-strand conformational polymorphism (SSCP) to determine apolipoprotein E (Apo E) genotypes in 47 individuals. A 295-base-pair (bp) DNA fragment coding for amino acid residues 80-178 of the Apo E protein gave distinct patterns for the three alleles. When we used SSCP to determine the Apo E polymorphism of five individuals whose phenotyping results differed from those of genotyping, the SSCP results agreed with the genotyping results obtained by the PCR-based amplification refractory mutation system (ARMS). Because most of the reported rare alleles of the Apo E gene involve mutations of amino acid residues in positions 120-160, our SSCP method is useful for determining rare as well as common alleles.

scholarly journals Analysis of Coinfections with A/H1N1 Strain Variants among Pigs in Poland by Multitemperature Single-Strand Conformational Polymorphism

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Krzysztof Lepek ◽  
Beata Pajak ◽  
Lukasz Rabalski ◽  
Kinga Urbaniak ◽  
Krzysztof Kucharczyk ◽  
...  
Keyword(s):  
Influenza A ◽  
Cost Effective ◽  
Single Strand ◽  
Surveillance Systems ◽  
Monitoring And Control ◽  
Influenza A Viruses ◽  
Single Strand Conformational Polymorphism ◽  
Conformational Polymorphism ◽  
Wide Range ◽  
Different Strains

Monitoring and control of infections are key parts of surveillance systems and epidemiological risk prevention. In the case of influenza A viruses (IAVs), which show high variability, a wide range of hosts, and a potential of reassortment between different strains, it is essential to study not only people, but also animals living in the immediate surroundings. If understated, the animals might become a source of newly formed infectious strains with a pandemic potential. Special attention should be focused on pigs, because of the receptors specific for virus strains originating from different species, localized in their respiratory tract. Pigs are prone to mixed infections and may constitute a reservoir of potentially dangerous IAV strains resulting from genetic reassortment. It has been reported that a quadruple reassortant, A(H1N1)pdm09, can be easily transmitted from humans to pigs and serve as a donor of genetic segments for new strains capable of infecting humans. Therefore, it is highly desirable to develop a simple, cost-effective, and rapid method for evaluation of IAV genetic variability. We describe a method based on multitemperature single-strand conformational polymorphism (MSSCP), using a fragment of the hemagglutinin (HA) gene, for detection of coinfections and differentiation of genetic variants of the virus, difficult to identify by conventional diagnostic.

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