scholarly journals Detection of exceedingly low levels of HIV proviral DNA in multimillion peripheral blood mononuclear cells by PCR.

1993 ◽  
Vol 3 (1) ◽  
pp. 63-64 ◽  
Author(s):  
P G Babu ◽  
B D Rawal ◽  
H Khayam-Bashi ◽  
G N Vyas
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Proviral Dna ◽  
Blood Mononuclear Cells ◽  
Low Levels ◽  
Hiv Proviral Dna

scholarly journals Identification of African Swine Fever Virus Transcription within Peripheral Blood Mononuclear Cells of Acutely Infected Pigs

Viruses ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2333
Author(s):  
Ann Sofie Olesen ◽  
Miyako Kodama ◽  
Louise Lohse ◽  
Francesc Accensi ◽  
Thomas Bruun Rasmussen ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Peripheral Blood Mononuclear ◽  
Virus Transcription ◽  
Blood Mononuclear Cells ◽  
Low Levels

African swine fever virus (ASFV) has become widespread in Europe, Asia and elsewhere, thereby causing extensive economic losses. The viral genome includes nearly 200 genes, but their expression within infected pigs has not been well characterized previously. In this study, four pigs were infected with a genotype II strain (ASFV POL/2015/Podlaskie); blood samples were collected before inoculation and at both 3 and 6 days later. During this period, a range of clinical signs of infection became apparent in the pigs. From the blood, peripheral blood mononuclear cells (PBMCs) were isolated. The transcription of the ASFV genes was determined using RNAseq on poly(A)+ mRNAs isolated from these cells. Only very low levels of virus transcription were detected in the PBMCs at 3 days post-inoculation (dpi) but, at 6 dpi, extensive transcription was apparent. This was co-incident with a large increase in the level of ASFV DNA within these cells. The pattern of the virus gene expression was very reproducible between the individual pigs. Many highly expressed genes have undefined roles. Surprisingly, some genes with key roles in virus replication were expressed at only low levels. As the functions of individual genes are identified, information about their expression becomes important for understanding their contribution to virus biology.

Fluctuation of HTLV-I proviral DNA in peripheral blood mononuclear cells of HTLV-I-associated myelopathy

1993 ◽  
Vol 42 (2) ◽  
pp. 147-154 ◽  
Author(s):  
Ryuji Kubota ◽  
Toshinobu Fujiyoshi ◽  
Shuji Izumo ◽  
Shinji Yashiki ◽  
Ikuro Maruyama ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Proviral Dna ◽  
Blood Mononuclear Cells
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