scholarly journals Empirical aspects of strand displacement amplification.

1993 ◽  
Vol 3 (1) ◽  
pp. 1-6 ◽  
Author(s):  
G T Walker
Keyword(s):  
Strand Displacement ◽  
Strand Displacement Amplification

scholarly journals Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring

Sensors ◽  
2021 ◽  
Vol 21 (2) ◽  
pp. 602
Author(s):  
Sandra Leonardo ◽  
Anna Toldrà ◽  
Mònica Campàs
Keyword(s):  
Food Safety ◽  
Environmental Monitoring ◽  
Rolling Circle Amplification ◽  
Dna Amplification ◽  
Isothermal Amplification ◽  
In Situ Monitoring ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Bacterial Detection ◽  
Efficient Detection

The easy and rapid spread of bacterial contamination and the risk it poses to human health makes evident the need for analytical methods alternative to conventional time-consuming laboratory-based techniques for bacterial detection. To tackle this demand, biosensors based on isothermal DNA amplification methods have emerged, which avoid the need for thermal cycling, thus facilitating their integration into small and low-cost devices for in situ monitoring. This review focuses on the breakthroughs made on biosensors based on isothermal amplification methods for the detection of bacteria in the field of food safety and environmental monitoring. Optical and electrochemical biosensors based on loop mediated isothermal amplification (LAMP), rolling circle amplification (RCA), recombinase polymerase amplification (RPA), helicase dependent amplification (HDA), strand displacement amplification (SDA), and isothermal strand displacement polymerisation (ISDPR) are described, and an overview of their current advantages and limitations is provided. Although further efforts are required to harness the potential of these emerging analytical techniques, the coalescence of the different isothermal amplification techniques with the wide variety of biosensing detection strategies provides multiple possibilities for the efficient detection of bacteria far beyond the laboratory bench.

Ultra-sensitive MicroRNA-21 detection based on multiple cascaded strand displacement amplification and CRISPR/Cpf1 (MC-SDA/CRISPR/Cpf1)

2021 ◽  
Author(s):  
Xiaolong Chen ◽  
Yuanyi Deng ◽  
Gaihua Cao ◽  
Yifan Xiong ◽  
Danqun Huo ◽  
...  
Keyword(s):  
Cancer Diagnosis ◽  
Analytical Method ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Diagnosis And Prognosis ◽  
Potential Biomarker ◽  
Cancer Diagnosis And Prognosis

MicroRNA-21 (miR-21) has been considered as a potential biomarker for cancer diagnosis and prognosis due to its highly expressed in tumors. Here, an analytical method which integrates the multiple cascaded...

Fluorometric determination of microRNA based on strand displacement amplification and rolling circle amplification

2017 ◽  
Vol 184 (11) ◽  
pp. 4359-4365 ◽  
Author(s):  
Yunlei Zhou ◽  
Bingchen Li ◽  
Minghui Wang ◽  
Jun Wang ◽  
Huanshun Yin ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Strand Displacement ◽  
Fluorometric Determination ◽  
Strand Displacement Amplification ◽  
Rolling Circle

scholarly journals Strand displacement amplification—an isothermal,in vitroDNA amplification technique

1992 ◽  
Vol 20 (7) ◽  
pp. 1691-1696 ◽  
Author(s):  
G. Terrance Walker ◽  
Melinda S. Fraiser ◽  
James L. Schram ◽  
Michael C. Little ◽  
James G. Nadeau ◽  
...  
Keyword(s):  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Amplification Technique
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