scholarly journals ZNF91 deletion in human embryonic stem cells leads to ectopic activation of SVA retrotransposons and up-regulation of KRAB zinc finger gene clusters

2021 ◽  
Author(s):  
Nina L. Haring ◽  
Elisabeth J. van Bree ◽  
Whitney S. Jordaan ◽  
Judith R.E. Roels ◽  
Gonzalo Congrains Sotomayor ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Zinc Finger ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Gene Clusters ◽  
Zinc Finger Gene ◽  
Ectopic Activation

scholarly journals Gene Editing of Human Embryonic Stem Cells via an Engineered Baculoviral Vector Carrying Zinc-finger Nucleases

2011 ◽  
Vol 19 (5) ◽  
pp. 942-950 ◽  
Author(s):  
Yuning Lei ◽  
Chi-Lin Lee ◽  
Kye-Il Joo ◽  
Jonathan Zarzar ◽  
Yarong Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Zinc Finger ◽  
Human Embryonic Stem Cells ◽  
Gene Editing ◽  
Embryonic Stem ◽  
Zinc Finger Nucleases

scholarly journals Generation of hematopoietic repopulating cells from human embryonic stem cells independent of ectopic HOXB4 expression

2005 ◽  
Vol 201 (10) ◽  
pp. 1603-1614 ◽  
Author(s):  
Lisheng Wang ◽  
Pablo Menendez ◽  
Farbod Shojaei ◽  
Li Li ◽  
Frederick Mazurier ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Ectopic Expression ◽  
Hematopoietic Cells ◽  
Embryonic Stem ◽  
Gene Expression Pattern ◽  
Gene Clusters ◽  
Hematopoietic Stem

Despite the need for alternative sources of human hematopoietic stem cells (HSCs), the functional capacity of hematopoietic cells generated from human embryonic stem cells (hESCs) has yet to be evaluated and compared with adult sources. Here, we report that somatic and hESC-derived hematopoietic cells have similar phenotype and in vitro clonogenic progenitor activity. However, in contrast with somatic cells, hESC-derived hematopoietic cells failed to reconstitute intravenously transplanted recipient mice because of cellular aggregation causing fatal emboli formation. Direct femoral injection allowed recipient survival and resulted in multilineage hematopoietic repopulation, providing direct evidence of HSC function. However, hESC-derived HSCs had limited proliferative and migratory capacity compared with somatic HSCs that correlated with a distinct gene expression pattern of hESC-derived hematopoietic cells that included homeobox (HOX) A and B gene clusters. Ectopic expression of HOXB4 had no effect on repopulating capacity of hESC-derived cells. We suggest that limitations in the ability of hESC-derived HSCs to activate a molecular program similar to somatic HSCs may contribute to their atypical in vivo behavior. Our study demonstrates that HSCs can be derived from hESCs and provides an in vivo system and molecular foundation to evaluate strategies for the generation of clinically transplantable HSC from hESC lines.

scholarly journals Zinc finger E-box–binding homeobox 1 (ZEB1) is required for neural differentiation of human embryonic stem cells

2018 ◽  
Vol 293 (50) ◽  
pp. 19317-19329 ◽  
Author(s):  
Yuan Jiang ◽  
Long Yan ◽  
Longkuo Xia ◽  
Xiaoyin Lu ◽  
Wenliang Zhu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Zinc Finger ◽  
Human Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
E Box

Resistance to zinc finger nuclease (ZFN)-mediated FANCA disruption in human embryonic stem cells (hESC)

2013 ◽  
Vol 41 (8) ◽  
pp. S29
Author(s):  
Kim Vanuytsel ◽  
Laura Ordovas Vidal ◽  
Qing Cai ◽  
Nisha Nair ◽  
Rob Van Rossom ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Zinc Finger ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Zinc Finger Nuclease

scholarly journals Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

10.3791/51764 ◽  
2014 ◽  
Author(s):  
Brigham J. Hartley ◽  
Stewart A. Fabb ◽  
Ben A.L. Finnin ◽  
John M. Haynes ◽  
Colin W. Pouton
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Targeting ◽  
Zinc Finger ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Zinc Finger Nuclease
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