scholarly journals Antisense RNAs during early vertebrate development are divided in groups with distinct features

2021 ◽  
pp. gr.262964.120
Author(s):  
Sanjana Pillay ◽  
Hazuki Takahashi ◽  
Piero Carninci ◽  
Aditi Kanhere
Keyword(s):  
Vertebrate Development ◽  
Antisense Rnas ◽  
Distinct Features

scholarly journals Antisense ncRNAs during early vertebrate development are divided in groups with distinct features

2020 ◽  
Author(s):  
Sanjana Pillay ◽  
Hazuki Takahashi ◽  
Piero Carninci ◽  
Aditi Kanhere
Keyword(s):  
Gene Expression Regulation ◽  
Expression Patterns ◽  
Vertebrate Development ◽  
Protein Coding ◽  
Antisense Rnas ◽  
Protein Coding Genes ◽  
Antisense Lncrnas ◽  
Group 2 ◽  
Group 1

ABSTRACTLong non-coding RNAs or lncRNAs are a broad class of non-protein coding RNAs that are >200nucleotides in length. A number of lncRNAs are shown to play an important role in gene expression regulation. LncRNAs antisense to a protein-coding gene can act either as positive or negative regulators of overlapping protein-coding mRNAs. Almost 50% of lncRNAs present during development of vertebrates such as zebrafish are of antisense lncRNA class. However, their role in gene expression regulation during development remains enigmatic. To understand the role of antisense lncRNAs in early vertebrate development, we took a computational biology approach to analyze existing as well as novel dataset. Our analysis of RNA sequencing data from zebrafish development indicates that antisense RNAs can be divided into two major classes based on their positive or negative co-expression patterns to the sense protein-coding genes. The ones with negative co-expression patterns or group-1 are maternal antisense lncRNAs that overlap mainly developmental genes. Group-2 with positive expression pattern overlap mainly house-keeping genes. Group-1 antisense lncRNAs are longer and more stable as compared to antisense lncRNAs in group-2. In addition, to answer if antisense RNAs in the two groups are differently localized in cell compartments, we deep-sequenced RNA from cytoplasmic and nuclear compartments during early developmental stages. The analysis of these compartment specific datasets revealed group-1 lncRNAs are cytosolic. Based on the cytosolic nature of group-1 RNAs and their higher complementarity to the overlapping developmental mRNAs, we speculate that the group-1 RNAs might function similar to microRNAs in silencing spurious expression of developmental genes. Group-1 and group-2 RNAs are also distinct in terms of their genomic configuration, conservation, length and transcriptional regulation. These results are not only important in understanding the role of antisense RNAs in development but also for predicting the nature of association between antisense lncRNA and overlapping protein-coding genes.

THE CHANCELLERY OF CONTRACT AFFAIRS (TO THE HISTORY OF LEGISLATIVE AND ADMINISTRATIVE REGULATION OF PUBLIC PROCUREMENT IN RUSSIA IN PETER THE GREAT’S TIME)

2020 ◽  
Vol 9 (3) ◽  
pp. 131-144
Author(s):  
D.A. REDIN ◽  
Keyword(s):  
Public Procurement ◽  
Armed Forces ◽  
Early History ◽  
Regulatory Body ◽  
Peter The Great ◽  
History Of ◽  
The Creation ◽  
Administrative Regulation ◽  
The Right ◽  
Distinct Features

The purpose of the article is to research the history of creation and formation of the Chancellery of Contract Affairs – the first supervisory and regulatory body in the field of public procurement in Russia. The early history of the Contracting Chancellery (1715–1717) can be traced in the context of the development of legislative and administrative regulation of public procurement during the reign of Peter the Great. The institution of public procurement itself, according to the author, is associated with the acquisition of distinct features of the modern state by Russia, which was manifested in the previous time. The immediate impetus for the development of the institution was the reform of the armed forces and the resulting mobilization efforts of the supreme power. The very content of the research predetermined the use of source-based and historical-legal methods. As a result of the study, the author states that the creation of a special body – the Chancellery of Contract Affairs, designed to take control of the situation under state contracts, turned out to be the right decision. The well-coordinated work of the Contracting Chancellery with the Senate, fiscal authorities and investigative bodies led to the creation of a number of important regulatory legal acts, almost ‘from scratch’ forming the legislative basis for the institution of public procurement functioning. The need for further work on the designated topic is noted.

scholarly journals Biology and Physics of Heterochromatin-Like Domains/Complexes

Cells ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1881
Author(s):  
Prim B. Singh ◽  
Stepan N. Belyakin ◽  
Petr P. Laktionov
Keyword(s):  
Phase Separation ◽  
Fission Yeast ◽  
Thermodynamic Description ◽  
Dynamic Structure ◽  
Vertebrate Development ◽  
Type Region ◽  
Additional Tool ◽  
3D Genome ◽  
Chromatin Template ◽  
Cis And Trans

The hallmarks of constitutive heterochromatin, HP1 and H3K9me2/3, assemble heterochromatin-like domains/complexes outside canonical constitutively heterochromatic territories where they regulate chromatin template-dependent processes. Domains are more than 100 kb in size; complexes less than 100 kb. They are present in the genomes of organisms ranging from fission yeast to human, with an expansion in size and number in mammals. Some of the likely functions of domains/complexes include silencing of the donor mating type region in fission yeast, preservation of DNA methylation at imprinted germline differentially methylated regions (gDMRs) and regulation of the phylotypic progression during vertebrate development. Far cis- and trans-contacts between micro-phase separated domains/complexes in mammalian nuclei contribute to the emergence of epigenetic compartmental domains (ECDs) detected in Hi-C maps. A thermodynamic description of micro-phase separation of heterochromatin-like domains/complexes may require a gestalt shift away from the monomer as the “unit of incompatibility” that determines the sign and magnitude of the Flory–Huggins parameter, χ. Instead, a more dynamic structure, the oligo-nucleosomal “clutch”, consisting of between 2 and 10 nucleosomes is both the long sought-after secondary structure of chromatin and its unit of incompatibility. Based on this assumption we present a simple theoretical framework that enables an estimation of χ for domains/complexes flanked by euchromatin and thereby an indication of their tendency to phase separate. The degree of phase separation is specified by χN, where N is the number of “clutches” in a domain/complex. Our approach could provide an additional tool for understanding the biophysics of the 3D genome.

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