scholarly journals Synchronized replication of genes encoding the same protein complex in fast-proliferating cells

2019 ◽  
Vol 29 (12) ◽  
pp. 1929-1938 ◽  
Author(s):  
Ying Chen ◽  
Ke Li ◽  
Xiao Chu ◽  
Lucas B. Carey ◽  
Wenfeng Qian
Keyword(s):  
Protein Complex ◽  
Proliferating Cells ◽  
Genes Encoding

scholarly journals Selection for synchronized replication of genes encoding the same protein complex during tumorigenesis

2018 ◽  
Author(s):  
Ying Chen ◽  
Ke Li ◽  
Xiao Chu ◽  
Lucas B. Carey ◽  
Wenfeng Qian
Keyword(s):  
Cell Cycle ◽  
Cancer Cells ◽  
Protein Complex ◽  
Embryonic Stem ◽  
Replication Timing ◽  
S Phase ◽  
Proliferating Cells ◽  
Genes Encoding ◽  
Dosage Balance ◽  
Selection For

ABSTRACTDNA replication alters the dosage balance among genes; at the mid-S phase, early-replicating genes have doubled their copies while late-replicating genes have not. Dosage imbalance among proteins, especially within members of a protein complex, is toxic to cells. Here, we propose the synchronized replication hypothesis: genes sensitive to stoichiometric relationships will be replicated simultaneously to maintain stoichiometry. In support of this hypothesis, we observe that genes encoding the same protein complex have similar replication timing, but surprisingly, only in fast-proliferating cells such as embryonic stem cells and cancer cells. The synchronized replication observed in cancer cells, but not in slow-proliferating differentiated cells, is due to convergent evolution during tumorigenesis that restores synchronized replication timing within protein complexes. Collectively, our study reveals that the selection for dosage balance during S phase plays an important role in the optimization of the replication-timing program; that this selection is relaxed during differentiation as the cell cycle is elongated, and restored as the cell cycle shortens during tumorigenesis.

Deregulated expression of the RanBP1 gene alters cell cycle progression in murine fibroblasts

1997 ◽  
Vol 110 (19) ◽  
pp. 2345-2357 ◽  
Author(s):  
A. Battistoni ◽  
G. Guarguaglini ◽  
F. Degrassi ◽  
C. Pittoggi ◽  
A. Palena ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Cell Cycle Progression ◽  
Protein Import ◽  
Proliferating Cells ◽  
Cycle Progression ◽  
Ran Gtpase ◽  
Genes Encoding ◽  
Active Transcription ◽  
Gtpase Cycle

RanBP1 is a molecular partner of the Ran GTPase, which is implicated in the control of several processes, including DNA replication, mitotic entry and exit, cell cycle progression, nuclear structure, protein import and RNA export. While most genes encoding Ran-interacting partners are constitutively active, transcription of the RanBP1 mRNA is repressed in non proliferating cells, is activated at the G1/S transition in cycling cells and peaks during S phase. We report here that forced expression of the RanBP1 gene disrupts the orderly execution of the cell division cycle at several stages, causing inhibition of DNA replication, defective mitotic exit and failure of chromatin decondensation during the telophase-to-interphase transition in cells that achieve nuclear duplication and chromosome segregation. These results suggest that deregulated RanBP1 activity interferes with the Ran GTPase cycle and prevents the functioning of the Ran signalling system during the cell cycle.

Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells

2002 ◽  
Vol 361 (3) ◽  
pp. 629-633 ◽  
Author(s):  
Makoto NISHIZUKA ◽  
Tomoko TSUCHIYA ◽  
Tsutomu NISHIHARA ◽  
Masayoshi IMAGAWA
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Adipocyte Differentiation ◽  
Early Stage ◽  
3T3 Cells ◽  
Subtraction Method ◽  
Proliferating Cells ◽  
Genes Encoding ◽  
Nih 3T3

Using a subtraction method, we have isolated genes that are induced early in the differentiation of mouse 3T3-L1 preadipocyte cells into adipocytes. These include the genes encoding transcription factors and signalling proteins, as well as unknown genes. Bach1, a transcription factor, and ARA70, a cofactor, were rapidly induced during differentiation. The induction of these two genes was observed only in growth-arrested 3T3-L1 cells, and not in proliferating cells. In NIH-3T3 cells, no induction was observed under either set of conditions. These results strongly indicate that Bach1 and ARA70 have valuable roles at the onset of adipocyte differentiation.

scholarly journals Cloning and characterization of the genes encoding the high-affinity iron-uptake protein complex Fet3/Ftr1 in the basidiomycete Phanerochaete chrysosporium

Microbiology ◽  
2007 ◽  
Vol 153 (6) ◽  
pp. 1772-1780 ◽  
Author(s):  
Luis F Larrondo ◽  
Paulo Canessa ◽  
Francisco. Melo ◽  
Rubén Polanco ◽  
Rafael. Vicuña
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Protein Complex ◽  
Iron Uptake ◽  
High Affinity ◽  
Genes Encoding

scholarly journals Stable Allele Frequency Distribution of the Plasmodium falciparum clag Genes Encoding Components of the High Molecular Weight Rhoptry Protein Complex

2012 ◽  
Vol 40 (3) ◽  
pp. 71-77 ◽  
Author(s):  
Jean Semé Fils Alexandre ◽  
Phonepadith Xangsayarath ◽  
Morakot Kaewthamasorn ◽  
Kazuhide Yahata ◽  
Jetsumon Sattabongkot ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Plasmodium Falciparum ◽  
High Molecular Weight ◽  
Allele Frequency ◽  
Frequency Distribution ◽  
Protein Complex ◽  
Allele Frequency Distribution ◽  
Genes Encoding ◽  
Rhoptry Protein

scholarly journals Metastatic Paragangliomas and Pheochromocytomas: An Epigenetic View

2021 ◽  
Author(s):  
María-Dolores Chiara ◽  
Lucía Celada ◽  
Andrés San José Martinez ◽  
Tamara Cubiella ◽  
Enol Álvarez-González ◽  
...  
Keyword(s):  
Histone Modification ◽  
Succinate Dehydrogenase ◽  
Protein Complex ◽  
Histone Demethylases ◽  
Epigenetic Landscape ◽  
Metastatic Cascade ◽  
Genes Encoding ◽  
Hereditary Tumors ◽  
Non Coding Rnas ◽  
Histone Modification Enzymes

Paragangliomas and pheochromocytoma (PPGLs) are hereditary tumors in about 40% of cases. Mutations in the genes encoding for components of the mitochondrial succinate dehydrogenase protein complex (SDHB, SDHD, SDHC) are among the most prevalent. Most PPGLs have a benign behavior, but patients with germline SDHB mutations may develop metastatic PPGLs in up to 30% of cases. This suggest that the SDH substrate, succinate, is key for the activation of the metastatic cascade. The last decade has witnessed significant advances in our understanding of how succinate may have oncogenic properties. It is now widely accepted that succinate is an oncometabolite that modifies the epigenetic landscape of SDH-deficient tumors via modulating the activities of DNA and histone modification enzymes. In this chapter, we summarize recent discoveries linking SDH-deficiency and metastasis in SDH-deficient PPGLs via inhibition of DNA methylcytosine dioxygenases, histone demethylases and modified expression of non-coding RNAs. We also highlight promising therapeutic avenues that may be used to counteract epigenetic deregulations.

scholarly journals Differential Protein Expression of Two Photosystem II Subunits, PsbO and PsbP, in an Albino Mutant of Bambusa edulis with Chloroplast DNA Aberration

2008 ◽  
Vol 133 (2) ◽  
pp. 270-277 ◽  
Author(s):  
Choun-Sea Lin ◽  
Nien-Tzu Liu ◽  
De-Chih Liao ◽  
Jau-Song Yu ◽  
Chuang-Hwei Tsao ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Protein Complex ◽  
Ribosomal Proteins ◽  
Light Signal ◽  
Two Dimensional ◽  
Differential Protein Expression ◽  
Albino Mutant ◽  
Genes Encoding ◽  
Oxygen Evolving ◽  
Post Transcriptional Regulation

The chloroplast genome of an albino mutant isolated from tissue culture of the bamboo Bambusa edulis Munro was examined to identify aberrations. A number of the chloroplast genes encoding ATP synthases, photosystem II subunits, NADH dehydrogenase, and ribosomal proteins had been deleted, at least partially, in the albino mutant. Comparison of the two-dimensional electrophoresis profiles of albino and green bamboos revealed three spots of reduced intensity, indicating repression of these proteins in the albino mutants. Mass spectroscopic analysis subsequently revealed that two of these proteins are 33-kDa subunits of the photosystem II oxygen-evolving protein complex (PsbO) and one is a 23-kDa subunit of photosystem II oxygen-evolving protein complex (PsbP). The genes encoding these two proteins were cloned from B. edulis, and were denoted BePsbO (accession no. EF669513) and BePsbP (accession no. EF669512). Reverse transcription polymerase chain reaction and two-dimensional gel analyses of BePsbO and BePsbP in green and albino bamboos grown in the light or dark revealed that the albino mutant, similar to its green counterpart, sensed the light signal, resulting in the induction of BePsbO and BePsbP transcription, but it did not accumulate the protein products. We conclude that the repression of protein-expressing BePsbO and BePsbP is because of a defect in post-transcriptional regulation in the albino mutant.

scholarly journals A Molybdopterin Oxidoreductase Is Involved in H2 Oxidation in Desulfovibrio desulfuricans G20

2009 ◽  
Vol 191 (8) ◽  
pp. 2675-2682 ◽  
Author(s):  
Xiangzhen Li ◽  
Qingwei Luo ◽  
Neil Q. Wofford ◽  
Kimberly L. Keller ◽  
Michael J. McInerney ◽  
...  
Keyword(s):  
Protein Complex ◽  
Parental Strain ◽  
Transmembrane Protein ◽  
Desulfovibrio Desulfuricans ◽  
Type I ◽  
Mutant Library ◽  
Putative Promoter ◽  
Genes Encoding ◽  
Tetraheme Cytochrome ◽  
H2 Oxidation

ABSTRACT Three mutants deficient in hydrogen/formate uptake were obtained through screening of a transposon mutant library containing 5,760 mutants of Desulfovibrio desulfuricans G20. Mutations were in the genes encoding the type I tetraheme cytochrome c 3 (cycA), Fe hydrogenase (hydB), and molybdopterin oxidoreductase (mopB). Mutations did not decrease the ability of cells to produce H2 or formate during growth. Complementation of the cycA and mopB mutants with a plasmid carrying the intact cycA and/or mopB gene and the putative promoter from the parental strain allowed the recovery of H2 uptake ability, showing that these specific genes are involved in H2 oxidation. The mop operon encodes a periplasm-facing transmembrane protein complex which may shuttle electrons from periplasmic cytochrome c 3 to the menaquinone pool. Electrons can then be used for sulfate reduction in the cytoplasm.

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