scholarly journals Noncoding transcription influences the replication initiation program through chromatin regulation

2018 ◽  
Vol 28 (12) ◽  
pp. 1882-1893 ◽  
Author(s):  
Julien Soudet ◽  
Jatinder Kaur Gill ◽  
Françoise Stutz
Keyword(s):  
Replication Initiation ◽  
Chromatin Regulation ◽  
Noncoding Transcription

A Novel Fluorescence-Based Screen for Inhibitors of the Initiation of DNA Replication in Bacteria

2019 ◽  
Vol 16 (3) ◽  
pp. 272-277 ◽  
Author(s):  
Rasmus N. Klitgaard ◽  
Anders Løbner-Olesen
Keyword(s):  
Dna Replication ◽  
High Throughput ◽  
Cyclic Peptide ◽  
Replication Initiation ◽  
False Positive Result ◽  
Over Expression ◽  
Initiator Protein ◽  
Dna Replication Initiation ◽  
Cellular Processes ◽  
Initiation Of Dna Replication

Background:One of many strategies to overcome antibiotic resistance is the discovery of compounds targeting cellular processes, which have not yet been exploited.Materials and Methods:Using various genetic tools, we constructed a novel high throughput, cellbased, fluorescence screen for inhibitors of chromosome replication initiation in bacteria.Results:The screen was validated by expression of an intra-cellular cyclic peptide interfering with the initiator protein DnaA and by over-expression of the negative initiation regulator SeqA. We also demonstrated that neither tetracycline nor ciprofloxacin triggers a false positive result. Finally, 400 extracts isolated mainly from filamentous actinomycetes were subjected to the screen.Conclusion:We concluded that the presented screen is applicable for identifying putative inhibitors of DNA replication initiation in a high throughput setup.

scholarly journals UV‐induced DNA damage disrupts the coordination between replication initiation, elongation, and completion

2020 ◽  
Author(s):  
Brian M. Wendel ◽  
Suzanne Hollingsworth ◽  
Charmain T. Courcelle ◽  
Justin Courcelle
Keyword(s):  
Dna Damage ◽  
Replication Initiation
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