scholarly journals Rapid molecular assays to study human centromere genomics

2017 ◽  
Vol 27 (12) ◽  
pp. 2040-2049 ◽  
Author(s):  
Rafael Contreras-Galindo ◽  
Sabrina Fischer ◽  
Anjan K. Saha ◽  
John D. Lundy ◽  
Patrick W. Cervantes ◽  
...  
Keyword(s):  
Molecular Assays ◽  
Human Centromere ◽  
Rapid Molecular Assays

scholarly journals Rapid molecular assays for detection of tuberculosis

Pneumonia ◽  
2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Rkia Eddabra ◽  
Hassan Ait Benhassou
Keyword(s):  
Molecular Assays ◽  
Rapid Molecular Assays

Differentiation of infectious bronchitis virus vaccine strains Ma5 and 4/91 by TaqMan real-time PCR

2017 ◽  
Vol 20 (3) ◽  
pp. 599-601 ◽  
Author(s):  
T. Stenzel ◽  
D. Dziewulska ◽  
M. Śmiałek ◽  
B. Tykałowski ◽  
J. Kowalczyk ◽  
...  
Keyword(s):  
Real Time ◽  
Virus Vaccine ◽  
Infectious Bronchitis Virus ◽  
Real Time Pcr ◽  
Cross Reactivity ◽  
Specific Primers ◽  
Assay Sensitivity ◽  
Infectious Bronchitis ◽  
Molecular Assays ◽  
Rapid Molecular Assays

Abstract The aim of this study was to develop rapid molecular assays for differentiating vaccine strains Ma5 and 4/91 of the infectious bronchitis virus (IBV). Specific primers and probes for S1 and N genes were designed based on the nucleotide sequences of both vaccine strains. Cross-reactivity was not observed. Assay sensitivity was 2.373 × 103 copies of the Ma5 strain, and 3.852 x 103 copies of the 4/91 strain. Samples belonging to a known genotype demonstrated that the designed assays supported rapid and sensitive detection of Ma5 and 4/91 vaccine strains of IBV.

scholarly journals Designing and evaluation of rapid molecular assays for first and second-line anti-tuberculosis drugs

2015 ◽  
Vol 4 ◽  
pp. 109
Author(s):  
M. Arjomandzadegan ◽  
A. Ahmadi ◽  
F. Salehi ◽  
V. Vahidi ◽  
T. Poolad ◽  
...  
Keyword(s):  
Second Line ◽  
Molecular Assays ◽  
Rapid Molecular Assays

scholarly journals A prospective evaluation of the analytical performance of GENECUBE® HQ SARS-CoV-2 and GENECUBE® FLU A/B

2021 ◽  
Author(s):  
Yoshihiko Kiyasu ◽  
Yusaku Akashi ◽  
Akio Sugiyama ◽  
Yuto Takeuchi ◽  
Shigeyuki Notake ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Analytical Performance ◽  
Rt Pcr ◽  
Influenza Virus A ◽  
Molecular Assays ◽  
Molecular Tests ◽  
Three Samples ◽  
Examination Time ◽  
Multiple Samples ◽  
Rapid Molecular Assays

AbstractBackgroundMolecular tests are the mainstay for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, their accessibility can be limited by the long examination time and inability to evaluate multiple samples at once. This study evaluated the analytical performance of the newly developed rapid molecular assays GENECUBE® HQ SARS-CoV-2 and the GENECUBE® FLU A/B.MethodThis prospective study was conducted between December 14, 2020, and January 9, 2021, at a polymerase chain reaction (PCR) center. Samples were collected from the nasopharynx with flocked swabs. Molecular tests were performed with the GENECUBE® system and reference reverse transcription (RT)-PCR, and the results of the two assays were compared.ResultAmong 1065 samples, 81 (7.6%) were positive for SARS-CoV-2 on the reference RT-PCR. Three showed discordance between GENECUBE® HQ SARS-CoV-2 and the reference RT-PCR; the total, positive and negative samples of concordance for the two assays were 99.7%, 100%, and 99.7%, respectively. All discordant cases were positive for GENECUBE® HQ SARS-CoV-2 and negative for the reference RT-PCR. SARS-CoV-2 was detected from all three samples by another molecular assay for SARS-CoV-2. For the GENECUBE® FLU A/B, the total, positive and negative samples of concordance for the two assays were 99.5%, 100%, and 99.1%.ConclusionThe GENECUBE® HQ SARS-CoV-2 and GENECUBE® FLU A/B demonstrated sufficient analytical performance to detect SARS-CoV-2 and influenza virus A/B.Key pointsWe prospectively evaluated the analytical performance of the newly developed rapid molecular assays GENECUBE® HQ SARS-CoV-2 and the GENECUBE® FLU A/B. The two assays showed >99% concordance rate compared with a reference PCR, which indicated their sufficient analytical performance to detect SARS-CoV-2 and influenza virus A/B.

scholarly journals Rapid Molecular Assays for the Detection of Yellow Fever Virus in Low-Resource Settings

2014 ◽  
Vol 8 (3) ◽  
pp. e2730 ◽  
Author(s):  
Camille Escadafal ◽  
Oumar Faye ◽  
Amadou Alpha Sall ◽  
Ousmane Faye ◽  
Manfred Weidmann ◽  
...  
Keyword(s):  
Yellow Fever ◽  
Yellow Fever Virus ◽  
Fever Virus ◽  
Low Resource Settings ◽  
Low Resource ◽  
Molecular Assays ◽  
Rapid Molecular Assays

scholarly journals Infectious Diseases (ID) Learning Unit: How Rapidly to Evaluate for Active Tuberculosis Disease in Low-Prevalence Settings

2016 ◽  
Vol 3 (2) ◽  
Author(s):  
Natasha Chida ◽  
Maunank Shah
Keyword(s):  
Practice Patterns ◽  
Infection Control Practice ◽  
Active Tuberculosis ◽  
Control Practice ◽  
Molecular Assays ◽  
Tb Diagnostics ◽  
Low Prevalence ◽  
Rapid Molecular Assays ◽  
Tuberculosis Disease ◽  
Review Current

Abstract With declining tuberculosis (TB) incidence in low-prevalence settings, many clinicians are likely unaware that the approach to diagnosing active TB is evolving with newer technologies. Rapid molecular assays are commercially available, and more are likely to enter the market in the coming years. These tests, such as the Xpert MTB/RIF, which can detect TB and drug-resistance in 2 hours, are increasingly used in settings with higher TB prevalence; however, uptake has been slower in low-prevalence settings. Newer algorithms incorporating rapid TB diagnostics have the ability to alter current clinical and infection control practice patterns. In this learning unit, we review current and newly available tests for the detection of active TB disease and their usage in low-prevalence settings.

scholarly journals 2293. Evaluation of Three Rapid Molecular Assays for the Detection of Group A Streptococcus

2018 ◽  
Vol 5 (suppl_1) ◽  
pp. S679-S680
Author(s):  
Eric Beck ◽  
Patricia Ross ◽  
Ashlee Clow ◽  
Jennifer Larson ◽  
Prina Patel ◽  
...  
Keyword(s):  
Group A Streptococcus ◽  
Molecular Assays ◽  
Group A ◽  
Rapid Molecular Assays
Sign in / Sign up

Export Citation Format

Share Document