scholarly journals Characterization of the Genomic Xist Locus in Rodents Reveals Conservation of Overall Gene Structure and Tandem Repeats but Rapid Evolution of Unique Sequence

2001 ◽  
Vol 11 (5) ◽  
pp. 833-849 ◽  
Author(s):  
T. B. Nesterova ◽  
S. Ya. Slobodyanyuk ◽  
E. A. Elisaphenko ◽  
A. I. Shevchenko ◽  
C. Johnston ◽  
...  
Keyword(s):  
Gene Structure ◽  
Tandem Repeats ◽  
Rapid Evolution ◽  
Unique Sequence ◽  
Xist Locus

Molecular cloning and characterization of rat karyopherin α1 gene: structure and expression

Gene ◽  
2004 ◽  
Vol 331 ◽  
pp. 149-157 ◽  
Author(s):  
Bingwei Wang ◽  
Zhihua Li ◽  
Lei Xu ◽  
Julian Goggi ◽  
Yi Yu ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Gene Structure ◽  
Gene Structure And Expression

scholarly journals Genome-Wide Identification and Characterization of the RCI2 Gene Family in Allotetraploid Brassica napus Compared with Its Diploid Progenitors

2022 ◽  
Vol 23 (2) ◽  
pp. 614
Author(s):  
Weiqi Sun ◽  
Mengdi Li ◽  
Jianbo Wang
Keyword(s):  
Brassica Napus ◽  
Gene Family ◽  
Gene Structure ◽  
Stress Responses ◽  
Stress Protein ◽  
Purifying Selection ◽  
Cis Acting ◽  
Genome Wide ◽  
Duplication Events

Brassica napus and its diploid progenitors (B. rapa and B. oleracea) are suitable for studying the problems associated with polyploidization. As an important anti-stress protein, RCI2 proteins widely exist in various tissues of plants, and are crucial to plant growth, development, and stress response. In this study, the RCI2 gene family was comprehensively identified and analyzed, and 9, 9, and 24 RCI2 genes were identified in B. rapa, B. oleracea, and B. napus, respectively. Phylogenetic analysis showed that all of the identified RCI2 genes were divided into two groups, and further divided into three subgroups. Ka/Ks analysis showed that most of the identified RCI2 genes underwent a purifying selection after the duplication events. Moreover, gene structure analysis showed that the structure of RCI2 genes is largely conserved during polyploidization. The promoters of the RCI2 genes in B. napus contained more cis-acting elements, which were mainly involved in plant development and growth, plant hormone response, and stress responses. Thus, B. napus might have potential advantages in some biological aspects. In addition, the changes of RCI2 genes during polyploidization were also discussed from the aspects of gene number, gene structure, gene relative location, and gene expression, which can provide reference for future polyploidization analysis.

The Drosophila pumilio gene: an unusually long transcription unit and an unusual protein

Development ◽  
1992 ◽  
Vol 114 (1) ◽  
pp. 221-232 ◽  
Author(s):  
P.M. Macdonald
Keyword(s):  
Amino Acid ◽  
Tandem Repeats ◽  
Repeat Unit ◽  
Transcription Unit ◽  
Posterior Pole ◽  
Single Amino Acid ◽  
Cdna Sequences ◽  
Subcortical Region ◽  
Mrna And Protein Expression

Specification of the posterior body plan in Drosophila requires the action of a determinant prelocalized to the posterior pole of the embryo. During embryogenesis this determinant appears to move anteriorly in a process dependent on the pumilio (pum) gene. This report describes the cloning and molecular characterization of a cDNA derived from the pum gene, and the analysis of pum mRNA and protein expression during early Drosophila development. The pum gene is unusually large; comparison of genomic and cDNA sequences reveals that the pum transcription unit is at least 160 kb in length. The pum cDNA encodes a 157 × 10(3) M(r) protein which consists mainly of regions enriched in a single amino acid, usually glycine, alanine, glutamine or serine/threonine. Six tandem repeats of a 36 amino acid repeat unit are also present. Pum protein is cytoplasmic and is concentrated in a subcortical region of the embryo. The distribution of pum protein exhibits no asymmetry along the anteroposterior axis of the embryo.

Isolation and characterization of tandem repeats in nematode genomes.

2021 ◽  
pp. 240-249
Author(s):  
Philippe Castagnone-Sereno
Keyword(s):  
Case Studies ◽  
Genetic Markers ◽  
Satellite Dna ◽  
Entomopathogenic Nematodes ◽  
Tandem Repeats ◽  
Genomic Resources ◽  
Isolation And Characterization ◽  
Nematode Genomes ◽  
Plant Parasitic

Abstract This chapter provides an overview of the practical methodologies that can be used to identify and characterize the tandem repeats that are most frequently used as genetic markers in nematodes (including plant-parasitic and entomopathogenic nematodes), namely satellite DNA and microsatellites. The objective is not to provide turnkey protocols, but rather to return to the main principles that govern these protocols. Case studies on nematodes will serve to illustrate the point. In that respect, two well-defined situations are to be considered, depending on whether genomic resources for the species under investigation are available or not.

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