scholarly journals Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases

2013 ◽  
Vol 24 (1) ◽  
pp. 125-131 ◽  
Author(s):  
Y. H. Sung ◽  
J. M. Kim ◽  
H.-T. Kim ◽  
J. Lee ◽  
J. Jeon ◽  
...  
Keyword(s):  
Gene Knockout ◽  
Highly Efficient ◽  
Efficient Gene

scholarly journals Achieving highly efficient gene transfer to the bladder by increasing the molecular weight of polymer-based nanoparticles

2021 ◽  
Vol 332 ◽  
pp. 210-224
Author(s):  
Gang Li ◽  
Shanshan He ◽  
Andreas G. Schätzlein ◽  
Robert M. Weiss ◽  
Darryl T. Martin ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Transfer ◽  
Highly Efficient ◽  
Efficient Gene

scholarly journals An Efficient, Rapid, and Recyclable System for CRISPR-Mediated Genome Editing in Candida albicans

mSphere ◽  
2017 ◽  
Vol 2 (2) ◽  
Author(s):  
Namkha Nguyen ◽  
Morgan M. F. Quail ◽  
Aaron D. Hernday
Keyword(s):  
Candida Albicans ◽  
Genome Editing ◽  
Fungal Pathogen ◽  
Gene Knockout ◽  
Strain Engineering ◽  
Molecular Genetic Analysis ◽  
Content Type ◽  
Highly Efficient ◽  
Discovery Research ◽  
Gene Knockouts

ABSTRACT Candida albicans is the most common fungal pathogen of humans. Historically, molecular genetic analysis of this important pathogen has been hampered by the lack of stable plasmids or meiotic cell division, limited selectable markers, and inefficient methods for generating gene knockouts. The recent development of clustered regularly interspaced short palindromic repeat(s) (CRISPR)-based tools for use with C. albicans has opened the door to more efficient genome editing; however, previously reported systems have specific limitations. We report the development of an optimized CRISPR-based genome editing system for use with C. albicans. Our system is highly efficient, does not require molecular cloning, does not leave permanent markers in the genome, and supports rapid, precise genome editing in C. albicans. We also demonstrate the utility of our system for generating two independent homozygous gene knockouts in a single transformation and present a method for generating homozygous wild-type gene addbacks at the native locus. Furthermore, each step of our protocol is compatible with high-throughput strain engineering approaches, thus opening the door to the generation of a complete C. albicans gene knockout library. IMPORTANCE Candida albicans is the major fungal pathogen of humans and is the subject of intense biomedical and discovery research. Until recently, the pace of research in this field has been hampered by the lack of efficient methods for genome editing. We report the development of a highly efficient and flexible genome editing system for use with C. albicans. This system improves upon previously published C. albicans CRISPR systems and enables rapid, precise genome editing without the use of permanent markers. This new tool kit promises to expedite the pace of research on this important fungal pathogen.

scholarly journals Cyclen-Based Cationic Lipids for Highly Efficient Gene Delivery towards Tumor Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e23134 ◽  
Author(s):  
Qing-Dong Huang ◽  
Guo-Xing Zhong ◽  
Yang Zhang ◽  
Jiang Ren ◽  
Yun Fu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Tumor Cells ◽  
Cationic Lipids ◽  
Highly Efficient ◽  
Efficient Gene

An inflammation self-adaptive nanocarrier for highly efficient gene therapy

2020 ◽  
Vol 17 ◽  
pp. 100287
Author(s):  
H. Jia ◽  
Y. Yang ◽  
M. Li ◽  
Y. Li ◽  
X. Han ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Highly Efficient ◽  
Efficient Gene ◽  
Self Adaptive

scholarly journals Efficient Gene Knockout in the Maize Pathogen Setosphaeria turcica Using Agrobacterium tumefaciens-Mediated Transformation

2013 ◽  
Vol 103 (6) ◽  
pp. 641-647 ◽  
Author(s):  
Chunsheng Xue ◽  
Dongliang Wu ◽  
Bradford J. Condon ◽  
Qing Bi ◽  
Weiwei Wang ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Molecular Mechanisms ◽  
Gene Knockout ◽  
Induction Medium ◽  
Test Case ◽  
Melanin Biosynthesis ◽  
Setosphaeria Turcica ◽  
Recombination Efficiency ◽  
Efficient Gene ◽  
Agrobacterium Cell

Setosphaeria turcica, a hemibiotrophic pathogenic dothideomycete, is the causal agent of Northern Leaf Blight of maize, which periodically causes significant yield losses worldwide. To explore molecular mechanisms of fungal pathogenicity and virulence to the host, an efficient targeted gene knockout transformation system using Agrobacterium tumefaciens was established with field collected strains. The starting materials, incubation time, induction medium type, Agrobacterium cell density, and method of co-incubation were optimized for deletion of 1,3,8-trihydroxynaphthalene reductase, a gene in the melanin biosynthesis pathway, as a test case. Four additional genes were deleted in two different S. turcica field isolates to confirm robustness of the method. One of these mutant strains was reduced in virulence compared with the wild-type strain when inoculated on susceptible maize. Transformation efficiency was ≈20 ± 3 transformants per 1× 106 germlings and homologous recombination efficiency was 33.3 to 100%.

scholarly journals A Simple, Rapid, and Highly Efficient Gene Expression System for Multiheme Cytochromes c

2001 ◽  
Vol 65 (1) ◽  
pp. 185-189 ◽  
Author(s):  
Kiyoshi OZAWA ◽  
Fumiko YASUKAWA ◽  
Yumiko FUJIWARA ◽  
Hideo AKUTSU
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Gene Expression System ◽  
Highly Efficient ◽  
Efficient Gene ◽  
Cytochromes C
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