scholarly journals Genomic characterization of the Bacillus cereus sensu lato species: Backdrop to the evolution of Bacillus anthracis

2012 ◽  
Vol 22 (8) ◽  
pp. 1512-1524 ◽  
Author(s):  
M. E. Zwick ◽  
S. J. Joseph ◽  
X. Didelot ◽  
P. E. Chen ◽  
K. A. Bishop-Lilly ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Genomic Characterization ◽  
Bacillus Cereus Sensu Lato

Pathogenicity and characterization of a novel Bacillus cereus sensu lato isolate toxic to the Mediterranean fruit fly Ceratitis capitata Wied.

2015 ◽  
Vol 126 ◽  
pp. 71-77 ◽  
Author(s):  
Luca Ruiu ◽  
Giovanni Falchi ◽  
Ignazio Floris ◽  
Maria Giovanna Marche ◽  
Maria Elena Mura ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
The Mediterranean ◽  
Bacillus Cereus Sensu Lato

Biochemical characterization of two chitinases from Bacillus cereus sensu lato B25 with antifungal activity against Fusarium verticillioides P03

2020 ◽  
Author(s):  
Estefanía Morales-Ruiz ◽  
Ricardo Priego-Rivera ◽  
Alejandro Miguel Figueroa-López ◽  
Jesús Eduardo Cazares-Álvarez ◽  
Ignacio E Maldonado-Mendoza
Keyword(s):  
Bacillus Cereus ◽  
Biochemical Characterization ◽  
Fusarium Verticillioides ◽  
Expression System ◽  
Phytopathogenic Fungi ◽  
Fungal Cell ◽  
Synthetic Pesticides ◽  
Bacillus Cereus Sensu Lato ◽  
Dual Substrate

Abstract Bacterial chitinases are a subject of intense scientific research due to their biotechnological applications, particularly their use as biological pesticides against phytopathogenic fungi as a green alternative to avoid the use of synthetic pesticides. Bacillus cereus sensu lato B25 is a rhizospheric bacterium that is a proven antagonist of Fusarium verticillioides, a major fungal pathogen of maize. This bacterium produces two chitinases that degrade the fungal cell wall and inhibit its growth. In this work, we used a heterologous expression system to purify both enzymes to investigate their biochemical traits in terms of Km, Vmax, optimal pH and temperature. ChiA and ChiB work as exochitinases, but ChiB exhibited a dual substrate activity and it is also an endochitinase. In this work, the direct addition of these chitinases inhibited fungal conidial germination and therefore they may play a major role in the antagonism against F. verticillioides.

scholarly journals Characterization of a Novel Lytic Protein Encoded by the Bacillus cereus E33L GeneampDas a Bacillus anthracis Antimicrobial Protein

2012 ◽  
Vol 78 (8) ◽  
pp. 3025-3027 ◽  
Author(s):  
Feliza A. Bourguet ◽  
Brian E. Souza ◽  
Angela K. Hinz ◽  
Matthew A. Coleman ◽  
Paul J. Jackson
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Antimicrobial Protein ◽  
Cell Wall Biosynthesis ◽  
Bacterial Genomes ◽  
Content Type ◽  
Highly Active ◽  
Low Concentrations

ABSTRACTLytic proteins encoded by bacterial genomes have been implicated in cell wall biosynthesis and recycling. TheBacillus cereusE33LampDgene encodes a putativeN-acetylmuramoyl-l-alanine amidase. This gene, expressedin vitro, produced a very stable, highly active lytic protein. Very low concentrations rapidly and efficiently lyse vegetativeBacillus anthraciscells.

scholarly journals Genomic characterization of three novel Basilisk-like phages infecting Bacillus anthracis

BMC Genomics ◽  
2018 ◽  
Vol 19 (1) ◽  
Author(s):  
J. Farlow ◽  
D. Bolkvadze ◽  
L. Leshkasheli ◽  
I. Kusradze ◽  
A. Kotorashvili ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Genomic Characterization

scholarly journals Complete Genome Sequence of Bacillus cereus Bacteriophage vB_BceS_KLEB30-3S

2020 ◽  
Vol 9 (20) ◽  
Author(s):  
Monika Šimoliūnienė ◽  
Deividas Tumėnas ◽  
Kotryna Kvederavičiūtė ◽  
Rolandas Meškys ◽  
Sigitas Šulčius ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Lake Ecosystem ◽  
Trna Genes ◽  
Temperate Bacteriophage ◽  
Content Type ◽  
Protein Encoding ◽  
Genomic Characterization ◽  
Karst Lake ◽  
Encoding Genes

In this study, we present the genomic characterization of the temperate bacteriophage vB_BceS_KLEB30-3S (KLEB30-3S), which was induced from Bacillus cereus strain KR3M-30, isolated from a gypsum karst lake ecosystem in Lithuania. The 37,134-bp genome of KLEB30-3S contains 58 predicted protein-encoding genes and no tRNA genes.

scholarly journals Rapid Microscopic Detection of Bacillus anthracis by Fluorescent Receptor Binding Proteins of Bacteriophages

2020 ◽  
Vol 8 (6) ◽  
pp. 934 ◽  
Author(s):  
Peter Braun ◽  
Immanuel Wolfschläger ◽  
Leonie Reetz ◽  
Lilia Bachstein ◽  
Ana Clara Jacinto ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Receptor Binding ◽  
Fusion Proteins ◽  
Binding Proteins ◽  
Fluorescent Proteins ◽  
Lambdoid Prophage ◽  
Unambiguous Detection ◽  
Close Genetic Relationship ◽  
Bacillus Cereus Sensu Lato

Bacillus anthracis, the etiological agent of anthrax disease, is typically diagnosed by immunological and molecular methods such as polymerase chain reaction (PCR). Alternatively, mass spectrometry techniques may aid in confirming the presence of the pathogen or its toxins. However, because of the close genetic relationship between B. anthracis and other members of the Bacillus cereus sensu lato group (such as Bacillus cereus or Bacillus thuringiensis) mis- or questionable identification occurs frequently. Also, bacteriophages such as phage gamma (which is highly specific for B. anthracis) have been in use for anthrax diagnostics for many decades. Here we employed host cell-specific receptor binding proteins (RBP) of (pro)-phages, also known as tail or head fibers, to develop a microscopy-based approach for the facile, rapid and unambiguous detection of B. anthracis cells. For this, the genes of (putative) RBP from Bacillus phages gamma, Wip1, AP50c and from lambdoid prophage 03 located on the chromosome of B. anthracis were selected. Respective phage genes were heterologously expressed in Escherichia coli and purified as fusions with fluorescent proteins. B. anthracis cells incubated with either of the reporter fusion proteins were successfully surface-labeled. Binding specificity was confirmed as RBP fusion proteins did not bind to most isolates of a panel of other B. cereus s.l. species or to more distantly related bacteria. Remarkably, RBP fusions detected encapsulated B. anthracis cells, thus RBP were able to penetrate the poly-γ-d-glutamate capsule of B. anthracis. From these results we anticipate this RBP-reporter assay may be useful for rapid confirmative identification of B. anthracis.

scholarly journals Germination and Characterization of Bacillus anthracis and Bacillus cereus in Nematodes

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Ann‐Desdemonia Neh Fowajuh ◽  
Ernesto Abel‐Santos
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Anthracis

scholarly journals Adaptation and phenotypic diversification of Bacillus thuringiensis 407 biofilm are accompanied by a fuzzy spreader morphotype

2021 ◽  
Author(s):  
Yicen Lin ◽  
Gergely Maróti ◽  
Mikael Lenz Strube ◽  
Ákos T. Kovács
Keyword(s):  
Bacillus Thuringiensis ◽  
Bacillus Cereus ◽  
Experimental Evolution ◽  
Insertion Sequence ◽  
Competitive Ability ◽  
Genetic Characterization ◽  
Phenotypic Diversification ◽  
Bacillus Cereus Sensu Lato ◽  
Adhesive Forces

AbstractBacillus cereus group (Bacillus cereus sensu lato) has a diverse ecology, including various species that produce biofilms on abiotic and biotic surfaces. While genetic and morphological diversification enable the adaptation of multicellular communities, this area remains largely unknown in the Bacillus cereus group. In this work, we dissected the experimental evolution of Bacillus thuringiensis 407 Cry-during continuous recolonization of plastic beads. We observed the evolution of a distinct colony morphotype that we named fuzzy spreader (FS) variant. Most multicellular traits of the FS variant displayed higher competitive ability versus the ancestral strain, suggesting an important role for diversification in the adaptation of B. thuringiensis to the biofilm lifestyle. Further genetic characterization of FS variant revealed the disruption of a guanylyltransferase gene by an insertion sequence (IS) element, which could be similarly observed in the genome of a natural isolate. The evolved FS and the deletion mutant in the guanylyltransferase gene (Bt407ΔrfbM) displayed similarly altered aggregation and hydrophobicity compared to the ancestor strain, suggesting that adaptation process highly depends on the physical adhesive forces.

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