scholarly journals H3K27me3 forms BLOCs over silent genes and intergenic regions and specifies a histone banding pattern on a mouse autosomal chromosome

2008 ◽  
Vol 19 (2) ◽  
pp. 221-233 ◽  
Author(s):  
F. M. Pauler ◽  
M. A. Sloane ◽  
R. Huang ◽  
K. Regha ◽  
M. V. Koerner ◽  
...  
Keyword(s):  
Banding Pattern ◽  
Autosomal Chromosome ◽  
Silent Genes ◽  
Intergenic Regions

scholarly journals Splicing factor USP39 promotes ovarian cancer malignancy through maintaining efficient splicing of oncogenic HMGA2

2021 ◽  
Vol 12 (4) ◽  
Author(s):  
Shourong Wang ◽  
Zixiang Wang ◽  
Jieyin Li ◽  
Junchao Qin ◽  
Jianping Song ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Malignant Tumors ◽  
Splicing Factor ◽  
Ovarian Cancer Cells ◽  
Nuclear Speckles ◽  
Aberrant Expression ◽  
Intergenic Regions

AbstractAberrant expression of splicing factors was found to promote tumorigenesis and the development of human malignant tumors. Nevertheless, the underlying mechanisms and functional relevance remain elusive. We here show that USP39, a component of the spliceosome, is frequently overexpressed in high-grade serous ovarian carcinoma (HGSOC) and that an elevated level of USP39 is associated with a poor prognosis. USP39 promotes proliferation/invasion in vitro and tumor growth in vivo. Importantly, USP39 was transcriptionally activated by the oncogene protein c-MYC in ovarian cancer cells. We further demonstrated that USP39 colocalizes with spliceosome components in nuclear speckles. Transcriptomic analysis revealed that USP39 deletion led to globally impaired splicing that is characterized by skipped exons and overrepresentation of introns and intergenic regions. Furthermore, RNA immunoprecipitation sequencing showed that USP39 preferentially binds to exon-intron regions near 5′ and 3′ splicing sites. In particular, USP39 facilitates efficient splicing of HMGA2 and thereby increases the malignancy of ovarian cancer cells. Taken together, our results indicate that USP39 functions as an oncogenic splicing factor in ovarian cancer and represents a potential target for ovarian cancer therapy.

scholarly journals Effects of Combination of Different −10 Hexamers and Downstream Sequences on Stationary-Phase-Specific Sigma Factor ςS-Dependent Transcription in Pseudomonas putida

2000 ◽  
Vol 182 (23) ◽  
pp. 6707-6713 ◽  
Author(s):  
Eve-Ly Ojangu ◽  
Andres Tover ◽  
Riho Teras ◽  
Maia Kivisaar
Keyword(s):  
Stationary Phase ◽  
Pseudomonas Putida ◽  
Sigma Factor ◽  
Sigma Factors ◽  
Deficient Mutant ◽  
Wild Type ◽  
In Vivo Experiments ◽  
Silent Genes ◽  
Rna Polymerase Holoenzyme

ABSTRACT The main sigma factor activating gene expression, necessary in stationary phase and under stress conditions, is ςS. In contrast to other minor sigma factors, RNA polymerase holoenzyme containing ςS (EςS) recognizes a number of promoters which are also recognized by that containing ς70 (Eς70). We have previously shown that transposon Tn4652 can activate silent genes in starvingPseudomonas putida cells by creating fusion promoters during transposition. The sequence of the fusion promoters is similar to the ς70-specific promoter consensus. The −10 hexameric sequence and the sequence downstream from the −10 element differ among these promoters. We found that transcription from the fusion promoters is stationary phase specific. Based on in vivo experiments carried out with wild-type and rpoS-deficient mutant P. putida, the effect of ςS on transcription from the fusion promoters was established only in some of these promoters. The importance of the sequence of the −10 hexamer has been pointed out in several published papers, but there is no information about whether the sequences downstream from the −10 element can affect ςS-dependent transcription. Combination of the −10 hexameric sequences and downstream sequences of different fusion promoters revealed that ςS-specific transcription from these promoters is not determined by the −10 hexameric sequence only. The results obtained in this study indicate that the sequence of the −10 element influences ςS-specific transcription in concert with the sequence downstream from the −10 box.

Fluorescent Banding Pattern and Species-Specific DNA Marker inRumex thyrsiflorusFingerh.

2012 ◽  
Vol 137 (1) ◽  
pp. 70-77 ◽  
Author(s):  
A. Grabowska-Joachimiak ◽  
D. Kwolek ◽  
A. Kula ◽  
P. Marciniuk
Keyword(s):  
Banding Pattern ◽  
Dna Marker ◽  
Fluorescent Banding ◽  
Species Specific

Isozyme Banding Pattern and Estimation of Genetic Diversity among Guinea Grass Germplasm

2006 ◽  
Vol 53 (2) ◽  
pp. 339-347 ◽  
Author(s):  
Abha Jain ◽  
A. K. Roy ◽  
P. Kaushal ◽  
D. R. Malaviya ◽  
S. N. Zadoo
Keyword(s):  
Genetic Diversity ◽  
Banding Pattern ◽  
Guinea Grass

scholarly journals Sequential GTG-RBA banding pattern in prometaphase chromosomes of cattle (Bos taurus L)

1991 ◽  
Vol 23 (4) ◽  
pp. 273 ◽  
Author(s):  
D Di Berardino ◽  
MB Lioi ◽  
D Matassino
Keyword(s):  
Banding Pattern ◽  
Bos Taurus
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