scholarly journals The KH-type RNA-binding protein PSI is required for Drosophila viability, male fertility, and cellular mRNA processing

2002 ◽  
Vol 16 (1) ◽  
pp. 72-84 ◽  
Author(s):  
E. Labourier
Keyword(s):  
Male Fertility ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mrna Processing

scholarly journals Integrative Regulatory Mapping Indicates that the RNA-Binding Protein HuR Couples Pre-mRNA Processing and mRNA Stability

2011 ◽  
Vol 43 (3) ◽  
pp. 327-339 ◽  
Author(s):  
Neelanjan Mukherjee ◽  
David L. Corcoran ◽  
Jeffrey D. Nusbaum ◽  
David W. Reid ◽  
Stoyan Georgiev ◽  
...  
Keyword(s):  
Mrna Stability ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mrna Processing

scholarly journals The protein that binds the 3' end of histone mRNA: a novel RNA-binding protein required for histone pre-mRNA processing.

1996 ◽  
Vol 10 (23) ◽  
pp. 3028-3040 ◽  
Author(s):  
Z F Wang ◽  
M L Whitfield ◽  
T C Ingledue ◽  
Z Dominski ◽  
W F Marzluff
Keyword(s):  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mrna Processing ◽  
Histone Mrna

scholarly journals RNA-binding protein Maca is crucial for gigantic male fertility factor gene expression, spermatogenesis, and male fertility, in Drosophila

PLoS Genetics ◽  
2021 ◽  
Vol 17 (6) ◽  
pp. e1009655
Author(s):  
Li Zhu ◽  
Ryuya Fukunaga
Keyword(s):  
Gene Expression ◽  
Male Fertility ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Large Male ◽  
Rna Recognition Motifs ◽  
Fertility Factor ◽  
Recognition Motifs ◽  
Successful Expression

During spermatogenesis, the process in which sperm for fertilization are produced from germline cells, gene expression is spatiotemporally highly regulated. In Drosophila, successful expression of extremely large male fertility factor genes on Y-chromosome spanning some megabases due to their gigantic intron sizes is crucial for spermatogenesis. Expression of such extremely large genes must be challenging, but the molecular mechanism that allows it remains unknown. Here we report that a novel RNA-binding protein Maca, which contains two RNA-recognition motifs, is crucial for this process. maca null mutant male flies exhibited a failure in the spermatid individualization process during spermatogenesis, lacked mature sperm, and were completely sterile, while maca mutant female flies were fully fertile. Proteomics and transcriptome analyses revealed that both protein and mRNA abundance of the gigantic male fertility factor genes kl-2, kl-3, and kl-5 (kl genes) are significantly decreased, where the decreases of kl-2 are particularly dramatic, in maca mutant testes. Splicing of the kl-3 transcripts was also dysregulated in maca mutant testes. All these physiological and molecular phenotypes were rescued by a maca transgene in the maca mutant background. Furthermore, we found that in the control genetic background, Maca is exclusively expressed in spermatocytes in testes and enriched at Y-loop A/C in the nucleus, where the kl-5 primary transcripts are localized. Our data suggest that Maca increases transcription processivity, promotes successful splicing of gigantic introns, and/or protects transcripts from premature degradation, of the kl genes. Our study identified a novel RNA-binding protein Maca that is crucial for successful expression of the gigantic male fertility factor genes, spermatogenesis, and male fertility.

scholarly journals An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling

2017 ◽  
Vol 31 (18) ◽  
pp. 1910-1925 ◽  
Author(s):  
Yoshika Hayakawa-Yano ◽  
Satoshi Suyama ◽  
Masahiro Nogami ◽  
Masato Yugami ◽  
Ikuko Koya ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Adhesion ◽  
Neural Stem Cells ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mrna Processing

scholarly journals Evolutionarily Conserved Polyadenosine RNA Binding Protein Nab2 Cooperates with Splicing Machinery To Regulate the Fate of Pre-mRNA

2016 ◽  
Vol 36 (21) ◽  
pp. 2697-2714 ◽  
Author(s):  
Sharon Soucek ◽  
Yi Zeng ◽  
Deepti L. Bellur ◽  
Megan Bergkessel ◽  
Kevin J. Morris ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Tail Length ◽  
Mrna Processing ◽  
Evolutionarily Conserved ◽  
Mrna Maturation

Numerous RNA binding proteins are deposited onto an mRNA transcript to modulate posttranscriptional processing events ensuring proper mRNA maturation. Defining the interplay between RNA binding proteins that couple mRNA biogenesis events is crucial for understanding how gene expression is regulated. To explore how RNA binding proteins control mRNA processing, we investigated a role for the evolutionarily conserved polyadenosine RNA binding protein, Nab2, in mRNA maturation within the nucleus. This study reveals thatnab2mutant cells accumulate intron-containing pre-mRNAin vivo. We extend this analysis to identify genetic interactions between mutant alleles ofnab2and genes encoding a splicing factor,MUD2, and RNA exosome,RRP6, within vivoconsequences of altered pre-mRNA splicing and poly(A) tail length control. As further evidence linking Nab2 proteins to splicing, an unbiased proteomic analysis of vertebrate Nab2, ZC3H14, identifies physical interactions with numerous components of the spliceosome. We validated the interaction between ZC3H14 and U2AF2/U2AF65. Taking all the findings into consideration, we present a model where Nab2/ZC3H14 interacts with spliceosome components to allow proper coupling of splicing with subsequent mRNA processing steps contributing to a kinetic proofreading step that allows properly processed mRNA to exit the nucleus and escape Rrp6-dependent degradation.

scholarly journals HuD, a neuronal-specific RNA-binding protein, is a putative regulator of N-myc pre-mRNA processing/stability in malignant human neuroblasts

Oncogene ◽  
1999 ◽  
Vol 18 (17) ◽  
pp. 2703-2710 ◽  
Author(s):  
Darina L Lazarova ◽  
Barbara A Spengler ◽  
June L Biedler ◽  
Robert A Ross
Keyword(s):  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mrna Processing ◽  
Processing Stability
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