scholarly journals Sequence requirements for efficient translational frameshifting in the Escherichia coli dnaX gene and the role of an unstable interaction between tRNA(Lys) and an AAG lysine codon.

1992 ◽  
Vol 6 (3) ◽  
pp. 511-519 ◽  
Author(s):  
Z Tsuchihashi ◽  
P O Brown
Keyword(s):  
Escherichia Coli ◽  
Translational Frameshifting ◽  
Sequence Requirements ◽  
Unstable Interaction

scholarly journals Function-Based Selection and Characterization of Base-Pair Polymorphisms in a Promoter of Escherichia coli RNA Polymerase-ς70

2001 ◽  
Vol 183 (9) ◽  
pp. 2866-2873 ◽  
Author(s):  
Jian Xu ◽  
Barbara C. McCabe ◽  
Gerald B. Koudelka
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Promoter Sequence ◽  
Kinetic Properties ◽  
Optimal Sequence ◽  
Sequence Requirements ◽  
Better Than

ABSTRACT We performed two sets of in vitro selections to dissect the role of the −10 base sequence in determining the rate and efficiency with which Escherichia coli RNA polymerase-ς70forms stable complexes with a promoter. We identified sequences that (i) rapidly form heparin-resistant complexes with RNA polymerase or (ii) form heparin-resistant complexes at very low RNA polymerase concentrations. The sequences selected under the two conditions differ from each other and from the consensus −10 sequence. The selected promoters have the expected enhanced binding and kinetic properties and are functionally better than the consensus promoter sequence in directing RNA synthesis in vitro. Detailed analysis of the selected promoter functions shows that each step in this multistep pathway may have different sequence requirements, meaning that the sequence of a strong promoter does not contain the optimal sequence for each step but instead is a compromise sequence that allows all steps to proceed with minimal constraint.

scholarly journals Role of aspartate 27 in the binding of methotrexate to dihydrofolate reductase from Escherichia coli.

1988 ◽  
Vol 263 (19) ◽  
pp. 9187-9198 ◽  
Author(s):  
J R Appleman ◽  
E E Howell ◽  
J Kraut ◽  
M Kühl ◽  
R L Blakley
Keyword(s):  
Escherichia Coli ◽  
Dihydrofolate Reductase
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