scholarly journals Egalitarian is a selective RNA-binding protein linking mRNA localization signals to the dynein motor

2009 ◽  
Vol 23 (13) ◽  
pp. 1546-1558 ◽  
Author(s):  
M. Dienstbier ◽  
F. Boehl ◽  
X. Li ◽  
S. L. Bullock
Keyword(s):  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mrna Localization ◽  
Dynein Motor

A staufen-like RNA-binding protein in translocation channels linking nurse cells to oocytes in Notonecta shows nucleotide-dependent attachment to microtubules

1999 ◽  
Vol 112 (17) ◽  
pp. 2947-2955
Author(s):  
S. Hurst ◽  
N.J. Talbot ◽  
H. Stebbings
Keyword(s):  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mrna Localization ◽  
Dependent Manner ◽  
Nurse Cells ◽  
Double Stranded Rna ◽  
Standard Procedures ◽  
Rna Probes ◽  
Peptide Antibody

In Drosophila melanogaster the staufen gene encodes an RNA-binding protein that is essential for the correct localization of certain nurse cell-derived transcripts in oocytes. Although the mechanism underlying mRNA localization is unknown, mRNA-staufen complexes have been shown to move in a microtubule-dependent manner, and it has been suggested that staufen associates with a motor protein which generates the movement. We have investigated this possibility using Notonecta glauca in which nurse cells also supply the oocytes with mRNA, but via greatly extended nutritive tubes comprised of large aggregates of parallel microtubules. Using a staufen peptide antibody and RNA probes we have identified a staufen-like protein, which specifically binds double-stranded RNA, in the nutritive tubes of Notonecta. We show that while the staufen-like protein does not co-purify with microtubules from ovaries using standard procedures it does so under conditions of motor-entrapment, specifically in the presence of AMP-PNP. We also show that the staufen-like protein is subsequently removed by ATP and GTP, but not ADP. Nucleotide-dependent binding to microtubules is typical of a motor-mediated interaction and the pattern of attachment and detachment of the staufen-like protein correlates with that of a kinesin protein within the ovaries. Our findings indicate that the staufen-like RNA-binding protein attaches to, and is transported along, Notonecta ovarian microtubules by a kinesin motor.

scholarly journals A highly conserved RNA-binding protein for cytoplasmic mRNA localization in vertebrates

1998 ◽  
Vol 8 (9) ◽  
pp. 489-496 ◽  
Author(s):  
James O. Deshler ◽  
Martin I. Highett ◽  
Tatiana Abramson ◽  
Bruce J. Schnapp
Keyword(s):  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mrna Localization

scholarly journals Yeast Mitochondrial Biogenesis: A Role for the PUF RNA-Binding Protein Puf3p in mRNA Localization

PLoS ONE ◽  
2008 ◽  
Vol 3 (6) ◽  
pp. e2293 ◽  
Author(s):  
Yann Saint-Georges ◽  
Mathilde Garcia ◽  
Thierry Delaveau ◽  
Laurent Jourdren ◽  
Stephane Le Crom ◽  
...  
Keyword(s):  
Mitochondrial Biogenesis ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mrna Localization

scholarly journals RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells

2018 ◽  
Vol 30 (10) ◽  
pp. 2529-2552 ◽  
Author(s):  
Li Tian ◽  
Hong-Li Chou ◽  
Laining Zhang ◽  
Seon-Kap Hwang ◽  
Shawn R. Starkenburg ◽  
...  
Keyword(s):  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mrna Localization ◽  
Rice Endosperm

scholarly journals Egalitarian binding partners, Dynein Light Chain and Bicaudal-D, act sequentially to link mRNA to the Dynein motor

2019 ◽  
Author(s):  
Chandler H. Goldman ◽  
Rajalakshmi Veeranan-Karmegam ◽  
Hannah Neiswender ◽  
Graydon B. Gonsalvez
Keyword(s):  
Light Chain ◽  
Rna Binding ◽  
Rna Binding Protein ◽  
Mrna Localization ◽  
Null Alleles ◽  
Dynein Light Chain ◽  
Binding Partners ◽  
Dynein Motor ◽  
Microtubule Motors

AbstractA widely conserved mechanism of polarity establishment is the localization of mRNA to specific cellular regions. While it is clear that many mRNAs are transported to their destinations along microtubule tracks, much less is known regarding the mechanism by which these mRNAs are linked to microtubule motors. The RNA binding protein Egalitarian (Egl) is necessary for localization of several mRNAs in Drosophila oocytes and embryos. In addition to binding RNA, Egl also interacts with Dynein light chain (Dlc) and Bicaudal-D (BicD). The role of Dlc and BicD in mRNA localization has remained elusive. Like Egl, both proteins are required for oocyte specification. Null alleles in these genes result in an oogenesis block. In this report, we used an innovative approach to overcome the oogenesis block. Our findings reveal that the primary function of Dlc is to promote Egl dimerization. Loss of dimerization compromises the ability of Egl to bind RNA. Consequently, Egl is not bound to cargo, and is not able to efficiently associate with BicD and the Dynein motor. Our results therefore identify the key molecular steps required for assembling a localization competent mRNP.

235: The RNA-Binding Protein IMP3: A Novel Molecular Marker Predicts Progression of Superficial (TA and T1) Urothelial Carcinomas of Bladder

2007 ◽  
Vol 177 (4S) ◽  
pp. 78-79
Author(s):  
Lioudmila Sitnikova ◽  
Gary Mendese ◽  
Qin Lui ◽  
Bruce A. Woda ◽  
Di Lu ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Urothelial Carcinomas
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