scholarly journals Mechanisms that regulate localization of a DNA double-strand break to the nuclear periphery

2009 ◽  
Vol 23 (8) ◽  
pp. 912-927 ◽  
Author(s):  
P. Oza ◽  
S. L. Jaspersen ◽  
A. Miele ◽  
J. Dekker ◽  
C. L. Peterson
Keyword(s):  
Nuclear Periphery ◽  
Strand Break ◽  
Double Strand Break ◽  
Dna Double Strand Break

scholarly journals Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region

2006 ◽  
Vol 172 (2) ◽  
pp. 189-199 ◽  
Author(s):  
Pierre Therizols ◽  
Cécile Fairhead ◽  
Ghislain G. Cabal ◽  
Auguste Genovesio ◽  
Jean-Christophe Olivo-Marin ◽  
...  
Keyword(s):  
Functional Organization ◽  
Nuclear Periphery ◽  
Strand Break ◽  
Double Strand Break ◽  
Subtelomeric Region ◽  
Genome Integrity ◽  
Nuclear Pore ◽  
Core Complex ◽  
Yeast Saccharomyces Cerevisiae ◽  
Dna Double Strand Break

In the yeast Saccharomyces cerevisiae that lacks lamins, the nuclear pore complex (NPC) has been proposed to serve a role in chromatin organization. Here, using fluorescence microscopy in living cells, we show that nuclear pore proteins of the Nup84 core complex, Nup84p, Nup145Cp, Nup120p, and Nup133p, serve to anchor telomere XI-L at the nuclear periphery. The integrity of this complex is shown to be required for repression of a URA3 gene inserted in the subtelomeric region of this chromosome end. Furthermore, altering the integrity of this complex decreases the efficiency of repair of a DNA double-strand break (DSB) only when it is generated in the subtelomeric region, even though the repair machinery is functional. These effects are specific to the Nup84 complex. Our observations thus confirm and extend the role played by the NPC, through the Nup84 complex, in the functional organization of chromatin. They also indicate that anchoring of telomeres is essential for efficient repair of DSBs occurring therein and is important for preserving genome integrity.

scholarly journals Correction: Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region

2006 ◽  
Vol 172 (6) ◽  
pp. 951-951
Author(s):  
Pierre Therizols ◽  
Cécile Fairhead ◽  
Ghislain G. Cabal ◽  
Auguste Genovesio ◽  
Jean-Christophe Olivo-Marin ◽  
...  
Keyword(s):  
Nuclear Periphery ◽  
Strand Break ◽  
Double Strand Break ◽  
Subtelomeric Region ◽  
Double Strand Break Repair ◽  
Dna Double Strand Break ◽  
Break Repair
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