scholarly journals Coordinated but physically separable interaction with H3K27-demethylase and H3K4-methyltransferase activities are required for T-box protein-mediated activation of developmental gene expression

2008 ◽  
Vol 22 (21) ◽  
pp. 2980-2993 ◽  
Author(s):  
S. A. Miller ◽  
A. C. Huang ◽  
M. M. Miazgowicz ◽  
M. M. Brassil ◽  
A. S. Weinmann
Keyword(s):  
Gene Expression ◽  
Developmental Gene ◽  
T Box ◽  
Developmental Gene Expression ◽  
Separable Interaction

scholarly journals T-bet's Ability To Regulate Individual Target Genes Requires the Conserved T-Box Domain To Recruit Histone Methyltransferase Activity and a Separate Family Member-Specific Transactivation Domain

2007 ◽  
Vol 27 (24) ◽  
pp. 8510-8521 ◽  
Author(s):  
Megan D. Lewis ◽  
Sara A. Miller ◽  
Michael M. Miazgowicz ◽  
Kristin M. Beima ◽  
Amy S. Weinmann
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Function Analysis ◽  
Histone Methyltransferase ◽  
Transactivation Domain ◽  
Developmental Gene ◽  
Methyltransferase Activity ◽  
T Box ◽  
Developmental Gene Expression ◽  
Expression Pathways

ABSTRACT Appropriate cellular differentiation and specification rely upon the ability of key developmental transcription factors to precisely establish gene expression patterns. These transcription factors often regulate epigenetic events. However, it has been unclear whether this is the only role that they play in functionally regulating developmental gene expression pathways or whether they also participate in downstream transactivation events at the same promoter. The T-box transcription factor family is important in cellular specification events in many developmental systems, and determining the molecular mechanisms by which this family regulates gene expression networks warrants attention. Here, we examine the mechanism by which T-bet, a critical T-box protein in the immune system, influences transcription. T-bet is both necessary and sufficient to induce permissive histone H3-K4 dimethyl modifications at the CXCR3 and IFN-γ promoters. A T-bet structure-function analysis revealed that the conserved T-box domain, with a small C-terminal portion, is required for recruiting histone methyltransferase activity to promoters. Interestingly, this function is conserved in the T-box family and is necessary, but not sufficient, to induce transcription, with an independent transactivation activity also required. The requirement for two separable functional activities may ultimately contribute to the stringent role for T-box proteins in establishing specific developmental gene expression pathways.

scholarly journals Maternal Style Selectively Shapes Amygdalar Development and Social Behavior in Rats Genetically Prone to High Anxiety

2015 ◽  
Vol 37 (3) ◽  
pp. 203-214 ◽  
Author(s):  
Joshua L. Cohen ◽  
Matthew E. Glover ◽  
Phyllis C. Pugh ◽  
Andrew D. Fant ◽  
Rebecca K. Simmons ◽  
...  
Keyword(s):  
Gene Expression ◽  
Psychological Health ◽  
Early Life ◽  
Developmental Trajectory ◽  
Emotional Behavior ◽  
Developmental Gene ◽  
Developmental Gene Expression ◽  
Maternal Style ◽  
Anxiety Depression ◽  
Cross Fostering

The early-life environment critically influences neurodevelopment and later psychological health. To elucidate neural and environmental elements that shape emotional behavior, we developed a rat model of individual differences in temperament and environmental reactivity. We selectively bred rats for high versus low behavioral response to novelty and found that high-reactive (bred high-responder, bHR) rats displayed greater risk-taking, impulsivity and aggression relative to low-reactive (bred low-responder, bLR) rats, which showed high levels of anxiety/depression-like behavior and certain stress vulnerability. The bHR/bLR traits are heritable, but prior work revealed bHR/bLR maternal style differences, with bLR dams showing more maternal attention than bHRs. The present study implemented a cross-fostering paradigm to examine the contribution of maternal behavior to the brain development and emotional behavior of bLR offspring. bLR offspring were reared by biological bLR mothers or fostered to a bLR or bHR mother and then evaluated to determine the effects on the following: (1) developmental gene expression in the hippocampus and amygdala and (2) adult anxiety/depression-like behavior. Genome-wide expression profiling showed that cross-fostering bLR rats to bHR mothers shifted developmental gene expression in the amygdala (but not hippocampus), reduced adult anxiety and enhanced social interaction. Our findings illustrate how an early-life manipulation such as cross-fostering changes the brain's developmental trajectory and ultimately impacts adult behavior. Moreover, while earlier studies highlighted hippocampal differences contributing to the bHR/bLR phenotypes, our results point to a role of the amygdala as well. Future work will pursue genetic and cellular mechanisms within the amygdala that contribute to bHR/bLR behavior either at baseline or following environmental manipulations.

scholarly journals A compendium of developmental gene expression in Lake Malawi cichlid fishes

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
R. F. Bloomquist ◽  
T. E. Fowler ◽  
J. B. Sylvester ◽  
R. J. Miro ◽  
J. T. Streelman
Keyword(s):  
Gene Expression ◽  
Lake Malawi ◽  
Developmental Gene ◽  
Cichlid Fishes ◽  
Developmental Gene Expression
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