scholarly journals Neuronal Signaling through Endocytosis

2014 ◽  
Vol 6 (2) ◽  
pp. a020669-a020669 ◽  
Author(s):  
K. E. Cosker ◽  
R. A. Segal
Keyword(s):  
Neuronal Signaling

scholarly journals Large Scale Expression Changes of Genes Related to Neuronal Signaling and Developmental Processes Found in Lateral Septum of Postpartum Outbred Mice

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e63824 ◽  
Author(s):  
Brian E. Eisinger ◽  
Changjiu Zhao ◽  
Terri M. Driessen ◽  
Michael C. Saul ◽  
Stephen C. Gammie
Keyword(s):  
Large Scale ◽  
Lateral Septum ◽  
Developmental Processes ◽  
Neuronal Signaling ◽  
Outbred Mice

scholarly journals Role of 20‐HETE in neuronal signaling and contribution to neonatal hypoxic‐ischemic encephalopathy

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Raymond C Koehler ◽  
Zeng-Jin Yang ◽  
Erin L Carter ◽  
Kathleen K Kibler ◽  
Herman Kwansa ◽  
...  
Keyword(s):  
Hypoxic Ischemic Encephalopathy ◽  
Neuronal Signaling ◽  
Ischemic Encephalopathy

scholarly journals Automated classification of synaptic vesicles in electron tomograms of C. elegans using machine learning

2018 ◽  
Author(s):  
Kristin Verena Kaltdorf ◽  
Maria Theiss ◽  
Sebastian Matthias Markert ◽  
Mei Zhen ◽  
Thomas Dandekar ◽  
...  
Keyword(s):  
Machine Learning ◽  
Synaptic Vesicles ◽  
Egg Laying ◽  
Dense Core ◽  
Dense Core Vesicles ◽  
Ground Truth Data ◽  
Neuronal Signaling ◽  
C Elegans ◽  
Morphological Criteria ◽  
Combine Machine

1.AbstractSynaptic vesicles (SVs) are a key component of neuronal signaling and fulfil different roles depending on their composition. In electron micrograms of neurites, two types of vesicles can be distinguished by morphological criteria, the classical “clear core” vesicles (CCV) and the typically larger “dense core” vesicles (DCV), with differences in electron density due to their diverse cargos. Compared to CCVs, the precise function of DCVs is less defined. DCVs are known to store neuropeptides, which function as neuronal messengers and modulators [1]. In C. elegans, they play a role in locomotion, dauer formation, egg-laying, and mechano- and chemosensation [2]. Another type of DCVs, also referred to as granulated vesicles, are known to transport Bassoon, Piccolo and further constituents of the presynaptic density in the center of the active zone (AZ), and therefore are important for synaptogenesis [3].To better understand the role of different types of SVs, we present here a new automated approach to classify vesicles. We combine machine learning with an extension of our previously developed vesicle segmentation workflow, the ImageJ macro 3D ART VeSElecT. With that we reliably distinguish CCVs and DCVs in electron tomograms of C. elegans NMJs using image-based features. Analysis of the underlying ground truth data shows an increased fraction of DCVs as well as a higher mean distance between DCVs and AZs in dauer larvae compared to young adult hermaphrodites. Our machine learning based tools are adaptable and can be applied to study properties of different synaptic vesicle pools in electron tomograms of diverse model organisms.2.Author summaryVesicles are important components of the cell, and synaptic vesicles are central for neuronal signaling. Two types of synaptic vesicles can be distinguished by electron microscopy: the classical “clear core” vesicles (CCVs) and the typically larger “dense core” vesicles (DCVs). The distinct appearance of vesicles is caused by their different cargos. To rapidly distinguish between both vesicle types, we present here a new automated approach to classify vesicles in electron tomograms. We combine machine learning with an extension of our previously developed vesicle segmentation workflow, an ImageJ macro, to reliably distinguish CCVs and DCVs using specific image-based features. The approach was trained and validated using data-sets that were hand curated by microscopy experts. Our technique can be transferred to more extensive comparisons in both stages as well as to other neurobiology questions regarding synaptic vesicles.

Spatial Organization and Diffusion in Neuronal Signaling

2012 ◽  
pp. 133-161 ◽  
Author(s):  
Sherry-Ann Brown ◽  
Raquell M. Holmes ◽  
Leslie M. Loew
Keyword(s):  
Spatial Organization ◽  
Neuronal Signaling ◽  
And Diffusion
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