scholarly journals Organellar Calcium Buffers

2011 ◽  
Vol 3 (3) ◽  
pp. a004069-a004069 ◽  
Author(s):  
D. Prins ◽  
M. Michalak
Keyword(s):  
Calcium Buffers

scholarly journals Computational Study Of The Effect Of Calcium Buffers On The Calcium Current Cooperativity Of Exocytosis

2009 ◽  
Vol 96 (3) ◽  
pp. 659a-660a
Author(s):  
Victor Matveev ◽  
Richard Bertram ◽  
Arthur Sherman
Keyword(s):  
Calcium Current ◽  
Computational Study ◽  
Calcium Buffers

scholarly journals Two Structural Motifs within Canonical EF-Hand Calcium-Binding Domains Identify Five Different Classes of Calcium Buffers and Sensors

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e109287 ◽  
Author(s):  
Konstantin Denessiouk ◽  
Sergei Permyakov ◽  
Alexander Denesyuk ◽  
Eugene Permyakov ◽  
Mark S. Johnson
Keyword(s):  
Calcium Binding ◽  
Structural Motifs ◽  
Binding Domains ◽  
Ef Hand ◽  
Calcium Buffers

Membrane properties of dentate gyrus granule cells: comparison of sharp microelectrode and whole-cell recordings

1992 ◽  
Vol 67 (5) ◽  
pp. 1346-1358 ◽  
Author(s):  
K. J. Staley ◽  
T. S. Otis ◽  
I. Mody
Keyword(s):  
Membrane Potential ◽  
Action Potential ◽  
Dentate Gyrus ◽  
Granule Cells ◽  
Dendritic Tree ◽  
Membrane Voltage ◽  
Membrane Properties ◽  
Whole Cell ◽  
Calcium Buffers ◽  
Whole Cell Recordings

1. Whole-cell and sharp electrode recordings from adult rat dentate gyrus GCs were performed in the 400-microns-thick hippocampal slice preparation maintained at 34 +/- 1 degrees C. Intrinsic membrane properties of granule cells (GCs) were evaluated with the use of a switching current-clamp amplifier. 2. With the whole-cell technique, the average resting membrane potential (RMP) of GCs was -85 mV when a potassium gluconate electrode solution was used versus -74 mV measured with potassium acetate-filled sharp microelectrodes. The membrane voltage response to injected current was linear over two membrane potential ranges, greater than 10 mV hyperpolarized from RMP and between 10 mV more negative than RMP and -62 mV. The average input resistances (RN) calculated over these ranges were 107 and 228 M omega in the whole-cell recordings versus 37 and 54 M omega in the sharp electrode recordings. There was no correlation between RMP and RN with either recording technique. The membrane time constant (tau m) determined at the RMP was 26.9 ms for whole-cell recordings and 13.9 ms for sharp electrode recordings. 3. There was no evidence of time-dependent changes in RMP, RN, and tau m in whole-cell recordings, although the slow inward rectification seen at hyperpolarized potentials decreased over 30-60 min. Addition of calcium buffers to the whole-cell recording solution did not result in a significant change in the average RMP, the average RN, or the average tau m. 4. Action potential threshold was comparable in whole-cell (-49 mV) and sharp electrode (-52 mV) recordings, but action potential amplitude was larger in whole-cell (126 mV) than in sharp electrode (106 mV) recordings. Spike frequency adaptation was present in the whole-cell recordings and could be abolished by addition of calcium buffers to the electrode solution. 5. We estimated rho, the ratio of dendritic to somatic conductance, to be 5.1 for the whole-cell records and 2.1 for sharp electrode recordings. The electrotonic length of the equivalent cylinder representing the cell processes was estimated to be 0.49 from the whole-cell data and 0.79 from the sharp electrode recordings. This implies that at rest there is only a 10% decrement in steady-state membrane voltage along the length of the dendrite due to shunting across the membrane resistance; small synaptic events occurring in the distal dendritic tree will therefore have a more substantial influence on the soma than previous analyses suggested.(ABSTRACT TRUNCATED AT 400 WORDS)

Cell Cycle Contraction Waves in Xenopus are Suppressed by Injecting Calcium Buffers

1992 ◽  
Vol 183 (2) ◽  
pp. 368-369
Author(s):  
A. L. Miller ◽  
R. A. Fluck ◽  
L. F. Jaffe
Keyword(s):  
Cell Cycle ◽  
Calcium Buffers
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