scholarly journals Description of Corynebacterium rouxii sp. nov., a novel member of the diphtheriae species complex

2019 ◽  
Author(s):  
Edgar Badell ◽  
Mélanie Hennart ◽  
Carla Rodrigues ◽  
Virginie Passet ◽  
Melody Dazas ◽  
...  
Keyword(s):  
Type Strain ◽  
Biochemical Analysis ◽  
Phylogenetic Analyses ◽  
Toxin Gene ◽  
The Novel ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Spectral Peaks ◽  
The Usa ◽  
Tof Ms

AbstractA group of six clinical isolates previously identified as Corynebacterium diphtheriae biovar Belfanti, isolated from human cutaneous or peritoneum infections and from one dog, were characterized by genomic sequencing, biochemical analysis and mass spectrometry (MALDI-TOF MS). The six isolates were negative for the diphtheria toxin gene. Phylogenetic analyses showed that the six isolates (including FRC0190T) are clearly demarcated from C. diphtheriae, C. belfantii, C. ulcerans and C. pseudotuberculosis. The average nucleotide identity of FRC0190T with C. diphtheriae NCTC 11397T was 92.6%, and was 91.8% with C. belfantii FRC0043T. C. diphtheriae subsp. lausannense strain CHUV2995T appeared to be a later heterotypic synonym of C. belfantii (ANI, 99.3%). Phenotyping data revealed an atypical negative or heterogeneous intermediate maltose fermentation reaction for the six isolates. MALDI-TOF MS differentiated the new group from the other Corynebacterium taxa by the presence of specific spectral peaks. rpoB sequences showed identity to atypical, maltose-negative C. diphtheriae biovar Belfanti isolates previously described from two cats in the USA. We propose the name Corynebacterium rouxii sp. nov. for the novel group, with FRC0190T (= CIP 111752T = DSM 110354T) as type strain.

Nachweis von Actinobacillus equuli ssp. equuli bei Saugferkeln mit eitriger Polyarthritis und Tendovaginitis

2020 ◽  
Vol 48 (01) ◽  
pp. 51-58
Author(s):  
Christiane Maul ◽  
Marcel Suchowski ◽  
Kristin Klose ◽  
Vladislav Antov ◽  
Martin Pfeffer ◽  
...  
Keyword(s):  
16S Rrna ◽  
Post Partum ◽  
Toxin Gene ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Molekularbiologische Methoden ◽  
Tof Ms

Zusammenfassung Actinobacillus equuli ssp. equuli ist ein opportunistischer Erreger des Pferdes, der u. a. als Auslöser der Fohlenfrühlähme bekannt ist. Beim Schwein gibt es vergleichsweise wenige Beschreibungen zu Erkrankungen bedingt durch diese gramnegative Bakterienspezies. Der Bericht beschreibt den Ausbruch einer Infektion in einem kombinierten Zuchtbetrieb mit angeschlossener Mast. Im September 2018 traten bei etwa einem Drittel neugeborener Saugferkel aus Würfen von Jung- und Altsauen folgende Symptome auf: Normal entwickelte Ferkel zeigten nach etwa 6–8 Stunden post partum umfangsvermehrte Gelenke in Zusammenhang mit gering- bis hochgradig ausgeprägten Stütz- und/oder Hangbeinlahmheiten. Die Ferkel wiesen ein reduziertes Allgemeinbefinden auf und befanden sich teilweise in Seitenlage. Innerhalb der ersten Lebenstage kam es zu einer erhöhten Mortalität. Bei 4 der zur Untersuchung eingesandten 7 Saugferkel (2./3. Lebenstag) erfolgte eine pathologisch-anatomische Untersuchung. Hauptbefunde waren eitrige Polyarthritiden und Tendovaginitiden. Bei einem Tier lagen zudem eitrige Entzündungsherde in Gehirn und Nieren vor. Bei der bakteriologischen Untersuchung ließ sich in 18 Lokalisationen (Gehirn, Gelenke, eitrig-abszedierende Veränderungen an Gliedmaßen) A. equuli ssp. equuli nachweisen, zum Teil in Reinkultur. Zur Identifizierung des Erregers wurden kulturell-biochemische Eigenschaften überprüft und ein Massenspektrometrie-Verfahren (MALDI-TOF-MS) angewendet. Als molekularbiologische Methoden dienten sowohl die Sequenzanalyse des 16S-rRNA-Gens als auch eine PCR zum Nachweis ausgewählter apx-Toxin-Gene sowie eine die beiden Subspezies differenzierende PCR. Die Eintragsquelle und die Erregerübertragung im Bestand konnten abschließend nicht aufgeklärt werden. Aus den Isolaten wurde eine bestandsspezifische Vakzine hergestellt und eingesetzt.

scholarly journals Staphylococcus argenteus: An emerging subclinical bovine mastitis pathogen in Thailand

2019 ◽  
Vol 12 (12) ◽  
pp. 1940-1944
Author(s):  
Natapol Pumipuntu
Keyword(s):  
Bovine Mastitis ◽  
Bacterial Pathogen ◽  
Technical Difficulty ◽  
The Novel ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Milk Samples ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Background and Aim: Staphylococcus argenteus is an emerging species of the Staphylococcus aureus complex. It has usually been misidentified as S. aureus by conventional methods and its characteristics. S. argenteus is potentially emerging in both humans and animals with an increasing global distribution. This study aimed to differentiate and identify S. argenteus from S. aureus collected and isolated from milk samples of subclinical bovine mastitis cases in Maha Sarakham Province, Northeastern Thailand. Materials and Methods: Forty-two isolates of S. aureus were studied from 132 individual milk samples collected from subclinical bovine mastitis cases of 15 dairy farms in three districts of Maha Sarakham, Thailand. The identification was confirmed by conventional and immune-agglutination methods. Fifteen representative isolates which were suspected as being S. argenteus were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Results: The result from MALDI-TOF MS confirmed that seven from 15 isolates were S. argenteus and eight isolates were S. aureus. Conclusion: This study indicated that MALDI-TOF MS used as an identification and classification method could accurately differentiate the novel species, S. argenteus, from the S. aureus complex which is usually misdiagnosed. In addition, the identification of S. argenteus seems to be very limited in technical difficulty despite the fact that it may be the important causative pathogen in bovine mastitis as well as a pathogenic bacterium in food and milk. Therefore, it is essential for both bovine medicine and veterinary public health to emphasize and recognize this bacterial pathogen as an emerging disease of staphylococcal bacteria that there is a need for further study of S. argenteus infections.

scholarly journals Phenotypic and Genotypic Characterization of Veterinary Vibrio cincinnatiensis Isolates

2020 ◽  
Vol 8 (5) ◽  
pp. 739 ◽  
Author(s):  
Claudia Jäckel ◽  
Jens Andre Hammerl ◽  
Huynh-Huong-Thao Arslan ◽  
Cornelia Göllner ◽  
Nicole vom Ort ◽  
...  
Keyword(s):  
Hemolytic Activity ◽  
Type Strain ◽  
Biochemical Properties ◽  
Human Isolate ◽  
Clinical Strain ◽  
Phenotypic Traits ◽  
Bioinformatics Analyses ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Vibrio cincinnatiensis is a halophilic species which has been found in marine and estuarine environments worldwide. The species is considered a rare pathogen for which the significance for humans is unclear. In this study, nine veterinary isolates were investigated that were obtained from domestic animals in Germany. The isolates were mostly recovered from abortion material of pigs, cattle, and horse (amnion or fetuses). One isolate was from a goose. A human clinical strain from a case of enteritis in Germany described in the literature was also included in the study. Whole-genome sequencing (WGS) of all isolates and MALDI-TOF MS (matrix-assisted-laser-desorption/ionization time-of-flight mass spectrometry) were performed to verify the species assignment. All strains were investigated for phenotypic traits including antimicrobial resistance (AMR), biochemical properties, and two virulence-associated phenotypes (hemolytic activity and resistance to human serum). WGS data and MS spectra confirmed that all veterinary isolates are closely related to the type strain V. cincinnatiensis NCTC12012. An exception was the human isolate from Germany which is related to the other isolates but could belong to another species. The isolates were similar in most biochemical phenotypes. Only one strain showed a very weak hemolytic activity against sheep erythrocytes, and serum resistance was intermediate in two strains. AMR phenotypes were more variable between the isolates. Resistances were observed against ß-lactams ampicillin and cefoxitin and against tetracycline and the sulfonamide antibiotics trimethoprim and sulfamethoxazole. Some acquired AMR genes were identified by bioinformatics analyses. WGS and MALDI-TOF MS data reveal a close relationship of the veterinary isolates and the type strain V. cincinnatiensis NCTC12012, which is a clinical human isolate. As the veterinary isolates of this study were mostly recovered from abortion material (amnions and fetuses), a zoonotic potential of the veterinary isolates seems possible.

scholarly journals USE OF MALDI-TOF MS METHOD FOR SPECIES DIFFERENTIATION OF NEMATODES (BY EXAMPLE OF STUDYING PROTEIN PROFILES OF ASCARIDATE AND DIROFILARIA)

2020 ◽  
pp. 267-275
Author(s):  
Nagorniy ◽  
A. Aleshukina ◽  
I. Aleshukina ◽  
Ermakova
Keyword(s):  
Proteomic Analysis ◽  
Protein Profile ◽  
Species Differentiation ◽  
Protein Profiles ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Spectral Peaks ◽  
Spectral Profiles ◽  
Tof Ms

The article presents the results of studying protein profiles of nematodes relevant for the south of Russia (dirofilaria and ascaridate) using proteomic analysis based on MALDI-TOF MS. Analysis of the protein extracts of dirofilaria and ascaridate showed spectra with high-intensity peaks in the range of 2–20 kDa, the quality of the spectra and the intensities of the spectral peaks were consistent for all samples of the same species. It was noted that reliably frequent peaks were observed in the spectra obtained from various dirofilariae (D. repens and D. immitis) in the range from 3400 to 6000 kDa, that characterize the entire genus of nematodes. In the range above 6000 kDa, the spectra differed by representative peaks, which allowed us to differentiate one species from another according to the protein profile. The spectral profiles obtained from the ascarid A. suum and A. lumbricoi proteins, unlike dirofilaria, showed almost complete homology of graphic images in the m/z range from 3000 kDa to 15 000 kDa. At the same time, protein peaks in the range of 3000–7300 kDa, typical for the entire genus Ascaris, are constant. Three main peaks in both species were found in m/z 13 000, 13 400 and 14 400. Thus, the method of proteomic analysis based on MALDI-TOFF MS can serve as an effective tool in studies of the taxonomic identification of the pathogen.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

HPTLC-MALDI/TOF/MS vs. HPLC-ESI/MS2 for identification to flavonoids in plant extracts

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
L Fougère ◽  
D Da Silva ◽  
E Destandau ◽  
C Elfakir
Keyword(s):  
Plant Extracts ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Infektionserreger beim Schwein – Analyse des kultivierbaren Mikrobioms mittels MALDI-TOF MS

2017 ◽  
Author(s):  
M Erhard ◽  
M Metzner ◽  
D Köhler-Repp ◽  
B Köhler ◽  
R Storandt
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Quantitative metabolomics of Iranian saffron based on their HPLC-DAD and MALDI-TOF-MS

2019 ◽  
Author(s):  
M Hooshyari ◽  
H Rezadoost ◽  
P Ghezellou ◽  
A Ghassempour
Keyword(s):  
Maldi Tof Ms ◽  
Quantitative Metabolomics ◽  
Maldi Tof ◽  
Tof Ms
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