scholarly journals Cell therapy as a new approach on hepatic fibrosis of murine model of Schistosoma mansoni infection

2019 ◽  
Author(s):  
N. ALsulami Muslimah
Keyword(s):  
Hepatic Fibrosis ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Liver Histopathology ◽  
Marrow Mesenchymal Stem Cells ◽  
And Function

AbstractSchistosomiasis is an acute and chronic disease caused by blood flukes (trematode worms) of the genus Schistosoma. Schistosomiasis is disease that are prevalent in or unique to tropical and subtropical regions. Previous studies have shown that the role of bone marrow mesenchymal stem cells (BMSCs) therapy in improvement of hepatic fibrosis. Therefore, the current study was designed to assess the therapeutic role of BMSCs in murine schistosomiasis mansoni. BMSCs derived male mice were intraperitoneal injected into female mice that received S. mansoni cercariae through subcutaneous route. Mice were divided into four groups: negative control group (noninfected non treated); positive control group (infected non treated); BMSCs treated group; and untreated group. Liver histopathology and immunohistochemically were evaluated. BMSC intraperitoneal injection resulted in a significant reduction in liver collagen, granuloma size, and significant increase in OV-6 expression in the Schistosomiasis treated mice group. There was overall improvement of the pathological changes of the liver. The findings support that BMSCs has a regenerative potential in the histopathology and function of the liver tissue by decreasing liver fibrosis.

scholarly journals Immunomodulatory and Anti-Inflammatory Potentials of Trigona Honey in the Therapy and Prevention against Respiratory Infection in Wistar Rats

2020 ◽  
Vol 11 (3) ◽  
pp. 2955-2962
Author(s):  
Ibrahim Khaled Al-kafaween ◽  
Abu Bakar Mohd Hilmi ◽  
Mohamed M. Soliman
Keyword(s):  
Respiratory Infection ◽  
Wistar Rats ◽  
Serum Levels ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Gene Expressions ◽  
Anti Inflammatory

Trigona honey (TH) is well known for its therapeutic characteristics. To date, the study of Trigona honey as a prophylactic or immune booster prior to the bacterial infection of the invivo model is not well covered. This study aims to investigate anti-inflammatory and immune activities in Wistar rats infected with respiratory infection following with Trigona honey. 25 Wistar rats were assigned to possitive groups, negative control group, positive control group was fed TH (5 g / kg body weight) orally, the untreated group was infected with Staphylococcus aureus to induce respiratory infection, the treated group has been infected with S. aureus followed by treatment with TH at a dose of 1.5 ×108 CFU / mL and the preventive group ingested TH one week before S. aureus infection. Blood was obtained for biochemical analysis. Lung tissues have been collected for molecular examination. The results showed a significant decrease in serum levels of ALT, AST, urea and creatinine in the preventive and treated groups, serum IgG increased significantly (P<0.05) in the preventive and treated groups, IFN-y increased in the preventive group while decreased in the treated group, and IL-8 increased in the treated group while decreased in the preventive group. The mRNA expression of AGP is up-regulated in the positive control, preventive and treated groups. The α2-MG, TNF-α , and mRNA expressions showed lower regulation after administration of TH in preventive and treated groups. The results show the ability of TH to counteract immune and inflammatory changes in serum levels and gene expressions.

scholarly journals PAPARAN EKSTRAK TERIPANG PASIR (Holothuria scabra) TERHADAP GAMBARAN HISTOPATOLOGI HATI MENCIT (Mus musculus)

2016 ◽  
Vol 2 (2) ◽  
pp. 160-169
Author(s):  
Yosep Matruty ◽  
Theopilus Watuguly
Keyword(s):  
Mus Musculus ◽  
Sea Cucumber ◽  
Normal Liver ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Holothuria Scabra ◽  
Liver Histopathology ◽  
Group I

Background: In Indonesia, traditionally sea cucumber (Holothuria scabra) is often used as a cure gout. The purpose of the research is to determine the effects of sea cucumber (Holothuria scabra) extracts against liver histopathology picture mice (Mus musculus). Method: Mice used were male mice as much as 15 tails and were divided into 5 groups. Each group consisted of three mice. Group I as a positive control given allopurino l0.1%. The second group was given distilled water as a negative control. Group III, IV and V as the treatment group was given sea cucumber extract with each concentration of 5%, a concentration of 10% and 15% concentration. Result: The results were analyzed quantitatively, showed that the administration of potassium bromate showed the presence of fatty and necrosis. But after being given a sea cucumber extract with a concentration of 10% and a concentration of 15% still showed the presence of inflammatory cells and remnants of fatty liver (same as the picture of liver histopathology in the positive control). At a concentration of 5% normal liver histopathology same as the picture of liver histopathology in the negative control. Conclution: Sea cucumbers with a concentration of 5% can be used as a gout medicine without any side effects for conditions onal liver function.

scholarly journals Evaluation of Antimalarial Properties of Ficus platyphylla Del Leaf Extract in Mice

2019 ◽  
pp. 1-8
Author(s):  
J. Omame ◽  
A. E. Amobonye ◽  
A. Y. Kabiru
Keyword(s):  
Body Weight ◽  
Plant Extract ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Crude Plant Extract ◽  
Different Doses

Aim: This study was aim at investigating the effect of crude petroleum ether leaf extract of Ficus platyphylla Del on Plasmodium berghei infected mice. Place and Duration of Study: This research was carried out at the department of biochemistry, Federal university of technology minna, Niger state Nigeria in 2014. Methodology: The crude plant extract of F. platyphylla was administered 72 hours at different doses post and pre infection for both the curative and prophylactic study respectively against residual infection. Mice were divided into 5 groups of 5 mice each, 3 of the groups where administered crude plants extract of F. platyphaylla at different doses (200, 400 and 600 mg/kg body weight) while the other two serve as negative and positive control group and were administered 0.5 ml and 50 mg/kg body weight respectively. Results: The extract at all doses produced significant (P<0.05) dose dependent chemo-suppressive activity with %  inhibition of 38%, 61%, 74% and 81.8% for curative studies and 36.0%, 38.5%, 49.5% and 63.4% for prophylactic studies against the parasites at doses of 200 mg/kgbw, 400 mg/kgbw, 600 mg/kgbw of the extract and 50 mg/kgbw of Artesunate. All doses of the extract increased the survival time of the infected mice compared to the negative control group that was administered 0.5 ml normal saline. The variation in the values of Packed Cell Volume (PCV) for treated group before and after extract administration was not significant at (P<0.05). The phytochemical screening of the plant extract showed the presence of tannin, saponin, flavonoids, terpenoids, steroids, anthroquinone and phenol. Conclusion: The result of this study shows that F. Platyphylla leaf extract exhibited some antiplasmodial activity that could be exploited for safe, effective and affordable antimalaria regimen.

scholarly journals Algal beta-(1,3)-glucan and its effect on infectious bursal disease vaccination in poultry

2019 ◽  
Vol 88 (6) ◽  
pp. 311-315
Author(s):  
V. Van Hamme ◽  
R. Spaepen ◽  
N. Smeets
Keyword(s):  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Infectious Bursal Disease ◽  
Control Group ◽  
Serological Response ◽  
Bursal Disease ◽  
Control And Prevention ◽  
Antibody Titers

Vaccination is used as a control and prevention tool for infectious bursal disease (IBDV) in poultry. A new strategy for improving vaccination efficiency is the use of in-feed immune modulating ingredients. The aim of this study was to test if the use of beta-(1,3)-glucans in feed can enhance IBDV vaccination. The trial was conducted in ROSS 308 broilers. In the study, a negative control group (not vaccinated, not supplemented), a positive control group (vaccinated, not supplemented) and a treated group (vaccinated, supplemented with beta-(1,3)-glucan) were included. All broilers, except the negative control, were orally vaccinated at 18 days of age with a live IBDV vaccine. Blood samples were taken at day 18 and 35 to measure antibody titers against IBDV. Serological analysis showed the presence of maternal derived antibodies at time of vaccination in some birds. The beta-(1,3)-glucan supplemented birds showed decreased CV% (coefficient of variation) and significantly increased average antibody titers compared to non-supplemented vaccinated birds. Additionally, the beta-(1,3)-glucan group showed increased number of birds with antibody titers above the titer threshold for protective immunity. The results obtained in the current trial clearly indicate that beta-(1,3)-glucan can increase seroconversion and serological response to IBDV vaccination.

scholarly journals PENGARUH PEMBERIAN MINYAK BUAH MERAH (Pandanus conoideus lam) TERHADAP KADAR GLUKOSA DARAH DAN MALONDIALDEHID SERUM MENCIT YANG DIINDUKSI ALOKSAN

2015 ◽  
Vol 34 (1) ◽  
pp. 79
Author(s):  
Husnil Kadri ◽  
Ezy Julianx Jarit ◽  
Erlina Rustam
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Reduce Blood Glucose ◽  
Fruit Oil

AbstrakTujuan penelitian ini adalah untuk menentukan efek pemberian minyak buah merah (pandanus conoideus lam) terhadap kadar glukosa darah dan malondialdehid (MDA) serum mencit yang diinduksi dengan aloksan. Induksi aloksan ini menyebabkan hiperglikemia yang dapat mencetuskan stres oksidatif. Minyak buah merah yang mengandung banyak antioksidan dapat mengendalikan stres oksidatif yang akhirnya dapat menurunkan kadar glukosa darah dan MDA serum.Penelitian eksperimental ini menggunakan 15 ekor mencit jantan yang diseleksi secara random. Hewan coba dibagi menjadi 3 kelompok masing-masing terdiri 5 ekor mencit, yaitu; kelompok kontrol negatif yang hanya diberikan makan dan minum, kelompok kontrol positif yang diinduksi aloksan, dan kelompok perlakuan (diberi minyak buah merah oral) yang diinduksi aloksan. Setelah perlakuan hari ke-14, darah mencit diambil untuk diperiksa kadar glukosa darah dan MDA serum.Hasil penelitian menunjukkan rerata kadar glukosa darah kelompok kontrol negatif adalah 141,20 ± 13,10 mg/dl. Rerata kadar glukosa darah kelompok kontrol positif adalah 266,00 ± 111,10 mg/dl. Rerata kadar glukosa darah kelompok perlakuan adalah 194,80 ± 27,00 mg/dl. Rerata kadar MDA serum kelompok kontrol negatif adalah 0,91 ± 0,10 nmol/ml. Rerata kadar MDA serum kelompok kontrol positif adalah 1,49 ± 0,34 nmol/ml. Rerata kadar MDA kelompok perlakuan adalah 0,97 ± 0,14 nmol/ml. Perbedaan bermakna kadar glukosa darah hanya antara kelompok kontrol negatif dengan kelompok perlakuan (p<0.05). Analisis statistik kadar MDA serum menunjukkan terdapat perbedaan bermakna antara kelompok kontrol positif dengan kelompok perlakuan (p< 0,05).Kesimpulan dari penelitian ini ialah minyak buah merah tidak mampu menurunkan kadar glukosa darah pada kelompok perlakuan, tetapi minyak buah merah mampu menurunkan kadar MDA serum pada kelompok perlakuan.Kata kunci: minyak buah merah, glukosa darah, MDA serum, mencit, aloksanARTIKEL PENELITIAN80AbstractThe objective of this studi was to determine the effect of red fruit oil (pandanus conoideus lam) on blood glucose and serum malondialdehyde in alloxan induced diabetic mice. Hyperglycemia in this mice could lead to oxidative stress. Red fruit oil contains high antioxidants that have capability to control oxidative stress, so blood glucose and serum MDA is reduced.This experimental study had been carried out to 15 rats (randomized group design) which divided into three group of five rats each, i.e. negative control, positive control (alloxan induced), and the group was treated by alloxan induced which followed by red fruit oil orally for 14 days. The result was analyzed by using one way Anova with confidence interval 95%.The result show that blood glucose in negative control was 141.20 ± 13.10 mg/dl. Blood glucose in positive control was 266.00 ± 111.10 mg/dl. Blood glucose in the group was treated by alloxan induced which followed by red fruit orally is 194.80 ± 27.00 mg/dl. The MDA level in negative control group is 0.91 ± 0.10 nmol/ml, positive control group is 1.49 ± 0.34 nmol/ml, and the group was treated by alloxan induced which followed by red fruit orally is 0.97 ± 0.14 nmol/ml. Blood glucose was significantly different between negative control and treated group (p<0.05). Serum MDA was significantly different between positive control group and the group was treated by alloxan induced which followed by red fruit orally (p<0.05).The conclusion is red fruit oil cannot reduce blood glucose level in the group was treated by alloxan induced which followed by red fruit orally, but it has capability to quench serum MDA level in this group.Key word: red fruit oil – blood glucose – serum MDA – mice – alloxan

scholarly journals The Role of Ceftazidime as a Photosensitizer in Human Erythrocytes Through Oxidative Stress Mechanism

2018 ◽  
Vol 10 (2) ◽  
pp. 128-32 ◽  
Author(s):  
Mashuri Mashuri ◽  
Mustofa Ruhullah ◽  
Bayu Diertama Putera ◽  
Vicky Pramudinta Mega ◽  
Fadillah Alma Putra ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbonyl Compounds ◽  
Superoxide Radical ◽  
Uv Light ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Groups

BACKGROUND: Ceftazidime was known to cause photosensitization reactions. However, how it plays the role remained unclear. In our study, we aim to investigate the photosensitization effect of ceftazidime in erythrocytes via oxidative stress.METHODS: Samples were divided into six different groups: negative control group, positive control group and four experimental groups with 10%, 20%, 30%, and 40% ceftazidime, respectively. The positive control and experimental groups were exposed to ultraviolet (UV)-light for 2 hours. Superoxide radical, malondialdehyde (MDA), carbonyl compounds (CC) and methemoglobin (Met-Hb) levels were then measured.RESULTS: The results showed a significant increased of superoxide radical, MDA, CC and Met-Hb levels in all experimental groups compared to both negative or positive control groups (p< 0.05).CONCLUSION: In conclusion, our study confirmed the role of ceftazidime as a photosensitizer in erythrocytes via the oxidative stress mechanisms.KEYWORDS: ceftazidime, oxidative stress, photosensitizer, photosensitization.

scholarly journals Protective Effect of Potential Probiotic Strains from Fermented Ethiopian Food against Salmonella Typhimurium DT104 in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Guesh Mulaw ◽  
Diriba Muleta ◽  
Anteneh Tesfaye ◽  
Tesfaye Sisay
Keyword(s):  
Survival Rate ◽  
Growth Inhibition ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Mixed Potential ◽  
Probiotic Strains

Salmonella is one of the most harmful pathogens responsible for foodborne outbreaks, illnesses and deaths. The aim of this study was to evaluate the effect of potentially probiotic strains against Salmonella Typhimurium DT104 in mice. The compatibility test among the selected potential probiotic strains (Lactobacillus plantarum K132, Lactobacillus paracasei K114 and Lactococcus lactis E124) using the cross-streaking method showed the absence of antagonism. The anti-Salmonella activities of coculture of the isolated potential probiotics in the form of mixed or single culture showed a remarkable anti-Salmonella activity with 96.50 to 100% growth inhibition. The combination of strains, which showed the highest growth inhibition rates against Salmonella Typhimurium DT104, was used to test their effect on the colonization of mice by Salmonella Typhimurium DT104. White albino male mice were pretreated with the mixed potential probiotics for 7 days and infected with Salmonella Typhimurium DT104 for 1 day. A total of 3 treatments were applied, during which the negative control group was treated with phosphate-buffered saline (PBS); a positive control group (typ) was challenged with Salmonella Typhimurium DT104 alone. The treated group (pro-typ) was pretreated with mixed potential probiotic culture and then infected with Salmonella Typhimurium DT104. The survival rate of mice and counts of Salmonella in feces were recorded. The survival rate of mice on day 21 after the oral challenge with Salmonella Typhimurium DT104 was significantly p<0.05 higher in the experimental pro-typ group (100% survival) compared with the positive control group (20% survival). The counts (colony-forming unit per ml) of Salmonella in feces were significantly lower p<0.05 for the pro-typ group compared to the typ group. The combination of potential probiotic strains was able to protect mice against Salmonella Typhimurium DT104 infection that demonstrates their potential to be used as probiotic cultures for the production of functional fermented products.

scholarly journals UJI EFEK ANALGESIK EKSTRAK KULIT BATANG POHON MATOA (Pometia pinnata) PADA MENCIT (Mus musculus)

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Rafly F. Lumintang ◽  
Jane Wuisan ◽  
Pemsy M. Wowor
Keyword(s):  
Mus Musculus ◽  
Analgesic Effect ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Bark Extract ◽  
Control Group ◽  
Group Iii ◽  
Group I

Abstract: Pain is an unpleasant sensation that derives from the complex neurochemical processes in the central and peripheral nervous systems. Side effects of drugs inter alia opioids and NSAIDs can cause serious disorders, therefore, it is necessary to find and develop other effective analgesic drugs with low toxicity. In Indonesia, matoa (Pometia pinnata) is known as a typical plant in Papua especially in West Papua. In some countries, parts of matoa plants are used as traditional medicine. This study aimed to determine the analgesic effect of matoa bark extract (Pometia pinnata) on mice Mus musculus. This was an experimental study. Nine male mice weighed 30 g were divided into 3 groups, each consisted of 3 mice. Group I, the negative control group, was given aquadest; group II, the positive control group, was given aspirin solution; and group III, the treated group, was given matoa bark extract. Analgesic effect was determined by counting the mice movements (licking their back legs or jumping) during 1 minute in a beaker with a temperature of 550C. The results showed that after 30 minutes the average number of movements of the treated group decreased from 22 times to 19.3 times, and continued to decrease until 1 movement after 120 minutes. Conclusion: Matoa bark extract showed analgesic effect on mice Mus musculus.Keywords: analgesic effect, matoa bark, miceAbstrak: Nyeri adalah sensasi yang tidak menyenangkan yang berasal dari proses neurokimia kompleks di sistem saraf pusat dan perifer. Opioid dan golongan AINS dapat menimbulkan efek samping yang cukup berat; oleh karena itu, diperlukan obat analgesik yang efektif dengan toksisitas rendah. Di Indonesia, matoa (Pometia pinnata) dikenal sebagai tanaman khas Papua terutama Papua Barat. Di beberapa negara, bagian-bagian dari tanaman matoa telah digunakan sebagai obat tradisional. Penelitian ini bertujuan untuk mengetahui efek analgesik ekstrak kulit batang pohon matoa (Pometia pinnata) pada mencit Mus musculus. Penelitian ini menggunakan metode eksperimental. Sembilan ekor mencit jantan dengan berat rata-rata 30 g dibagi atas 3 kelompok hewan uji yang masing-masing terdiri dari 3 ekor mencit. Kelompok I yaitu kelompok kontrol negatif diberikan akuades; kelompok II yaitu kelompok kontrol positif diberikan larutan aspirin; dan kelompok III yaitu kelompok perlakuan diberikan ekstrak kulit batang matoa. Efek analgesik berupa jumlah gerakan mencit selama 1 menit saat diletakan di dalam beker dengan suhu tetap 550C. Gerakan yang dihitung berupa gerakan menjilat kaki belakang atau meloncat. Hasil penelitian menunjukkan bahwa pada menit ke-30 terjadi penurunan rerata jumlah respon gerakan mencit dari 22 kali menjadi 19,3 kali yang terus berkurang hingga menit ke-120 dimana hanya terdapat 1 gerakan. Simpulan: Ekstrak kulit batang pohon matoa memiliki efek analgesik pada mencit Mus musculus.Kata kunci: efek analgesik, kulit batang matoa, mencit

scholarly journals Bone Marrow-derived Mesenchymal Stem Cells Reverse Hepatic Fibrosis, Improved Vascularity, and Attenuate the Apoptosis in Carbon Tetrachloride-induced Hepatic Fibrosis Experimental Rats

2021 ◽  
Vol 9 (A) ◽  
pp. 698-706
Author(s):  
Abeer Mostafa ◽  
Zainab Altaib ◽  
Walaa Sayed ◽  
Eman Rashwan ◽  
Ashraf Albrakati
Keyword(s):  
Liver Fibrosis ◽  
Hepatic Fibrosis ◽  
Inflammatory Diseases ◽  
Inflammatory Marker ◽  
Negative Control Group ◽  
Treated Group ◽  
Negative Control ◽  
Control Group ◽  
Liver Functions ◽  
Immunological Rejection

Background: Liver fibrosis is a sequel of different chronic inflammatory diseases. The most effective treatment for end-stage liver fibrosis is liver transplantation; but the shortage of donor organs, immunological rejection, surgical complications, and high medical costs limit the transplantation. That’s why we are in argent need to develop new strategies in treatment.  Objectives: to evaluate the role of MSCs in regenerating liver cells and reverse hepatic fibrosis. Materials and Methods: 30 Animals were randomly divided into three groups (10 animals each): group 1: a negative control; group 2: induced liver fibrosis (pathological control).; group 3: induced liver fibrosis that received undifferentiated BM MSCs (3×106 cells/ml intraperitoneally/single dose); The extent of fibrosis, vascularization, and inflammation and hepatic cell apoptosis were evaluated together with assessment of liver functions. Results: The MSCs treated group showed significant improvement of liver functions, and attenuation of fibrosis histopathologicaly and down regulate the expression of TGF ß versus the induced fibrosis group. inflammatory marker(TNF,IL-6) were down regulated and vascularity was restored in MSCs treated group  compared to CCL4 induced fibrosis rats.  Conclusion: MSCs provide promising therapeutic agents in treatment of liver fibrosis.

scholarly journals Gambaran Glukosa Darah Setelah Latihan Fisik pada Tikus Wistar Diabetes Melitus yang Diinduksi Aloksan

2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Ahmad Syukri Harahap ◽  
Rahmatina B. Herman ◽  
Eti Yerizel
Keyword(s):  
Blood Glucose ◽  
Physical Exercise ◽  
Fasting Blood Glucose ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Diabetes Melitus

AbstrakDiabetes Melitus telah dikategorikan sebagai penyakit global yang prevalensinya terus meningkat dan penyebab utama morbiditas dan mortalitas. Latihan fisik merupakan salah satu tatalaksana untuk mengontrol glukosa darah secara nonfarmakologis. Tujuan dari penelitian ini adalah untuk mengetahui gambaran kadar glukosa darah pada tikus Wistar diabetes melitus yang diinduksi aloksan setelah pemberian latihan fisik. Penelitian eksperimental ini menggunakan 18 ekor tikus Wistar dengan berat badan 150-200 gram yang dibagi menjadi tiga kelompok, yaitu 6 ekor kelompok kontrol negatif (K-), 6 ekor kelompok kontrol positif (K+) dengan induksi aloksan tanpa pemberian latihan fisik dan 6 ekor kelompok perlakuan (P) dengan induksi aloksan dan diberi latihan fisik selama 4 minggu. Pengukuran kadar glukosa darah dilakukan dengan glucometer merek Accu-Check. Hasil penelitian menunjukkan peningkatan rata-rata kadar glukosa darah puasa secara bermakna pada kelompok kontrol positif (K+) dan perlakuan (P) setelah periode induksi yaitu, pada kelompok kontrol negatif 84,83±6,88 mg/dl, kelompok kontrol positif 220,80±12,29 mg/dl, dan kelompok perlakuan 248,50±94,55 mg/dl (p<0,05). Setelah periode latihan fisik, terdapat penurunan rata-rata glukosa darah puasa secara bermakna pada kelompok kontrol positif (K+) dan Perlakuan (P), namun penurunan pada kelompok perlakuan (P) lebih besar secara bermakna dibandingkan dengan kelompok kontrol positif (K+), yaitu rata-rata glukosa darah puasa kelompok kontrol positif 192,00±12,00 mg/dl dan kelompok perlakuan 163,00±20,26 mg/dl (p<0,05). Kesimpulan hasil penelitian ini adalah terdapat penurunan kadar glukosa darah puasa setelah latihan fisik.Kata kunci: latihan fisik, glukosa darah, diabetes melitusAbstractDiabetes Melitus has been categorized as a global disease which have increasing prevalence and the mine cause of morbidity and mortality. Phyisical exercise is one of the nonpharmacological treatment to control blood glucose. The objective of this research was to determine the blood gucose levels of aloksan induced’s wistar rat after physical exercise.The research used 18 wistar rats 150-200 gram weight, divided into three groups, as six negative control group (K-), six positive control group inducing aloksan without physical exercise (K+) and six treated group inducing aloksan with physical exercise for four weeks (P). Blood glucose was measured by Accu Check’s glucometer.The result showed an increase in fasting blood glucose level significantly on positive control group (K-) and treated group (P) after induced period, which was 84,83±6,88 mg/dl on negative control group, 220,80±12,29 mg/dl on positive control group, and 248,50±94,55 mg/dl on treated group (p<0,05). After physical exercise period, there was a decrease in fasting blood significantly on K+ and P, but in P group have more greater than K+ group, 192,00±12,00 mg/dl on positive control group (K+) and 163,00±20,26 mg/dl on treated group (P).Conclusion of this research is a decrease in fasting blood glucose levels after phyisical exercise.Keywords: physical exercise, blood glucose, diabetes melitus

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