scholarly journals Enzymatic degradation of RNA causes widespread protein aggregation in cell and tissue lysates

2019 ◽  
Author(s):  
Johan Aarum ◽  
Claudia P Cabrera ◽  
Tania A Jones ◽  
Shiron Rajendran ◽  
Rocco Adiutori ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Protein Aggregation ◽  
Enzymatic Degradation ◽  
Protein Nucleic Acid ◽  
Relationship Of ◽  
The Relationship ◽  
Synthetic Oligonucleotides ◽  
Shed Light ◽  
Acid Structure ◽  
Lateral Sclerosis

ABSTRACTMost proteins in cell and tissue lysates are soluble. Here, we show that many of these proteins, including several that are implicated in neurodegenerative diseases, are maintained in a soluble and functional state by association with endogenous RNA, as degradation of RNA invariably leads to protein aggregation. We identify the importance of nucleic acid structure, with single-stranded pyrimidine-rich bulges or loops surrounded by double-stranded regions being particularly efficient in this role, revealing an apparent one-to-one protein-nucleic acid stoichiometry. The relationship of these findings to pathological protein aggregation is suggested by our discovery that protein aggregates isolated from brain tissue from Amyotrophic Lateral Sclerosis patients can be rendered soluble after refolding by both RNA and synthetic oligonucleotides. Together, these findings open new avenues for understanding the mechanism behind protein aggregation and shed light on how certain proteins remain soluble.

Fungal melanins: a review

1998 ◽  
Vol 44 (12) ◽  
pp. 1115-1136 ◽  
Author(s):  
M J Butler ◽  
A W Day
Keyword(s):  
Molecular Biology ◽  
Analytical Methods ◽  
Enzymatic Degradation ◽  
Fundamental Properties ◽  
Wide Range ◽  
Lignin Peroxidases ◽  
Fungal Melanin ◽  
Relationship Of ◽  
The Relationship ◽  
Fungal Melanins

The relationship of polyketide melanogenesis molecular biology to that of nonmelanin-producing pathways in a wide range of fungi and other organisms is discussed. Analytical methods and fundamental properties of melanins are discussed and fungal melanin properties are compared with those of animal and bacterial melanins. The enzymatic degradation of melanins by lignin peroxidases is described.Key words: fungal melanin, polyketide melanin, DHN melanin, melanin degradation, melanin properties, melanin analysis.

scholarly journals Internal control, reporting failures and lack of scientific methodology for reporting – Status of the Al- Bireh- Palestine municipality –: الرقابة الداخلية وإخفاقاتها في إعداد التقارير وغياب المنهجية العلمية لإعداد التقارير – حالة بلدية البيرة – فلسطين –

2021 ◽  
Vol 5 (13) ◽  
pp. 41-24
Author(s):  
Ziyad Said Al- Tawil Ziyad Said Al- Tawil
Keyword(s):  
Internal Control ◽  
Scientific Methodology ◽  
Course Objectives ◽  
Laws And Policies ◽  
Relationship Of ◽  
The Relationship ◽  
Shed Light

  The question that the research seeks to answer is the relationship of good supervisory administrative reports to achieving the goals of the organization, and helping managers to show the spirit of work interest in making their administrative decisions and taking those decisions away from their personal aims and interests. The scientific and applied importance of research appears in an attempt to shed light on the importance of internal control and its correct scientific and technical reports in developing and maximizing the benefit of the organization.   The researcher applies his hypotheses to the municipality of Al- Bireh- Palestine as an applied case and studies the monitoring reports from the beginning of 2013 until the beginning of 2020 and the violations that were shown or not shown by the supervisory reports in some of the decisions taken at the time and whether the weakness of these reports had a role in the growth and increase of violations and deviation from the regulations and the policies in place at the time or not. The research assumes in general that the more supervisory reports are prepared in a wrong way technically and administratively, with the knowledge or ignorance of the source of those reports, the more deviation the administration and its decisions are from the course, objectives, laws and policies set for this organization.

scholarly journals Oligonucleotide-based systems: DNA, microRNAs, DNA/RNA aptamers

2016 ◽  
Vol 60 (1) ◽  
pp. 27-35 ◽  
Author(s):  
Pawan Jolly ◽  
Pedro Estrela ◽  
Michael Ladomery
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Rna Aptamers ◽  
Locked Nucleic Acid ◽  
Synthetic Analogue ◽  
Dna And Rna ◽  
Naturally Occurring ◽  
Wide Range ◽  
Synthetic Oligonucleotides ◽  
Shed Light

There are an increasing number of applications that have been developed for oligonucleotide-based biosensing systems in genetics and biomedicine. Oligonucleotide-based biosensors are those where the probe to capture the analyte is a strand of deoxyribonucleic acid (DNA), ribonucleic acid (RNA) or a synthetic analogue of naturally occurring nucleic acids. This review will shed light on various types of nucleic acids such as DNA and RNA (particularly microRNAs), their role and their application in biosensing. It will also cover DNA/RNA aptamers, which can be used as bioreceptors for a wide range of targets such as proteins, small molecules, bacteria and even cells. It will also highlight how the invention of synthetic oligonucleotides such as peptide nucleic acid (PNA) or locked nucleic acid (LNA) has pushed the limits of molecular biology and biosensor development to new perspectives. These technologies are very promising albeit still in need of development in order to bridge the gap between the laboratory-based status and the reality of biomedical applications.

scholarly journals Toward a Systematic Theology of the Sanctuary – Part I

2018 ◽  
Vol 33 (2) ◽  
pp. 212-227
Author(s):  
John C. Peckham
Keyword(s):  
Systematic Theology ◽  
Fundamental Theology ◽  
Relationship Of ◽  
The Relationship ◽  
Shed Light ◽  
The Way

This article is part one of an essay that offers some preliminary thoughts regarding the relationship of the sanctuary and systematic theology, focusing on just a few aspects which expose the relationship between the two. This article considers the nature of theological systems, issues related to an Adventist system of theology, and the relationship between fundamental theology and the sanctuary in particular, with attention to some broad, competing views of the sanctuary that are integrally related to the way one conceives of broader theological principles. This sets the stage for the second article, which will conclude the essay by discussing a number of important systematic elements that shed light on a potential systematic theology of the sanctuary.

scholarly journals TOWARD A SYSTEMATIC THEOLOGY OF THE SANCTUARY—PART II

2020 ◽  
Vol 34 (1) ◽  
pp. 4-31
Author(s):  
John C. Peckham
Keyword(s):  
Systematic Theology ◽  
Fundamental Theology ◽  
Relationship Of ◽  
The Relationship ◽  
Shed Light ◽  
The Way

This article is part two of an essay that offers some preliminary thoughts regarding the relationship of the sanctuary and systematic theology, focusing on just a few aspects which expose the relationship between the two. The first article considered the nature of theological systems, issues related to an Adventist system of theology, and the relationship between fundamental theology and the sanctuary in particular, with attention to some broad, competing views of the sanctuary that are integrally related to the way one conceives of broader theological principles. The first article set the stage for this second article, which concludes the essay by discussing a number of important systematic elements that shed light on a potential systematic theology of the sanctuary

scholarly journals So Near, So Far: Emmanuel Levinas and Vladimir Jankélévitch

Religions ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 922
Author(s):  
Joëlle Hansel
Keyword(s):  
Comparative Analysis ◽  
Emmanuel Levinas ◽  
French Philosophy ◽  
Jewish Thought ◽  
Anti Semitism ◽  
Key Concepts ◽  
The Difference ◽  
Relationship Of ◽  
The Relationship ◽  
Shed Light

The purpose of my article is to shed light on the relationship of proximity and distance that linked two major figures of 20th-century French philosophy: Emmanuel Levinas and Vladimir Jankélévitch. This article presents a comparative study of their respective views on Metaphysics and Ethics. It also deals with their contribution to the reflection on the fact of “Being Jewish”, the theme that was at the center of the preoccupations of these two artisans of the renewal of Jewish thought in France after the Shoah. I conduct a comparative analysis between the key concepts of their philosophy: Levinas’ “There is” and “Otherness” and Jankélévitch’s “I-know-not-what” and “Ipseity”. I point out the difference between Levinas’ ethics of Otherness and Jankélévitch’s morality of paradox. In the section on “Being Jewish”, I highlight the crucial distinction they both made between racism and anti-Semitism and the very different meaning they gave to it.

Jesus and Judaism

2018 ◽  
Author(s):  
Tobias Nicklas
Keyword(s):  
Gospel Of John ◽  
Jewish Tradition ◽  
Christ Followers ◽  
Relationship Of ◽  
The Relationship ◽  
Shed Light

This chapter explores the relationship between Jesus and Judaism as described in gospel texts of the late first and second centuries. It addresses two questions: (1) To what extent is Jesus presented as a ‘Jewish’ character, or as related to characters depicted as representatives of ‘Judaism’? (2) To what extent is Jesus described as following, disobeying, or violating Jewish practices? Material is provided by the Gospel of John and the ‘unknown Gospel’ of Papyrus Egerton 2. The two evangelists describe Jesus’ relation to Judaism in different ways: while both remain in a frame shaped by Jewish tradition, John creates a boundary between his community and ‘the Jews’ with ‘their synagogue’, a boundary absent from the Egerton fragments in spite of their polemical tone. These divergent representations of Jesus’ relationship to Jewish characters/practices shed light on the relationship of the Christ-followers behind our texts to what we would call ‘Judaism.’

Fiscal Institution or Local Community?

2020 ◽  
pp. 155-168
Author(s):  
Lajos Berkes
Keyword(s):  
Local Community ◽  
Late Antique ◽  
Village Community ◽  
Village Life ◽  
Relationship Of ◽  
The Village ◽  
The Relationship ◽  
Early Islamic ◽  
Shed Light ◽  
Community Administration

The abundant papyrological evidence surviving from late antique Egypt (4–8th c.) includes thousands of documents in Greek and Coptic on village life. These sources shed light on aspects of rural realities barely known from other areas of the ancient Mediterranean. Village administration and government are especially well documented. Late antique villages in Egypt were organised in a fiscal community (koinon) which was collectively liable for the payments of the taxes incumbent on the village and the cultivation of their land. This institution was governed by a body of officials consisting of members of the village elite. This chapter discusses the relationship of the fiscal village community, administration and elite in Byzantine and early Islamic Egypt.

scholarly journals Neutron Star Populations at the Millenium

2000 ◽  
Vol 177 ◽  
pp. 573-578
Author(s):  
James Cordes
Keyword(s):  
Neutron Star ◽  
Birth Rate ◽  
Core Collapse ◽  
Radio Pulsars ◽  
X Ray ◽  
Yield Information ◽  
Relationship Of ◽  
The Relationship ◽  
Careful Comparison ◽  
Shed Light

AbstractIdentified neutron star (NS) classes evidently are determined by several intertwined features: kinematics of NS at their formation (spin and translational); magnetic field strength; and binary membership. I discuss the well-known classes of isolated and accreting NS while keeping in mind recent discoveries of magnetars, anomalous X-ray pulsars, and long-period radio pulsars. I summarize the results of several likelihood analyses on radio pulsars, which yield information on the velocity distribution, luminosity function, and birth rate of high-field radio pulsars. I review the evidence for the occurrence of momentum kicks at the time of NS birth. Discerning the relationship of the classic radio pulsars to the more exotic classes probably requires careful comparison of magnetic fields, kinematics and birthrates, a program for the next millenium. Exciting discoveries of classic pulsars will also be made: sub-millisecond pulsars, massive binaries in fast orbits and truly hyper-velocity pulsars that shed light on core-collapse processes in supernovae.

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