scholarly journals In vitro activity of beauvericin against all developmental stages of Sarcoptes scabiei

2019 ◽  
Author(s):  
Charbel Al Khoury ◽  
Nabil Nemer ◽  
Georges Nemer ◽  
Mazen Kurban ◽  
Charlotte Bernigaud ◽  
...  
Keyword(s):  
Human Fibroblast ◽  
Developmental Stages ◽  
Sarcoptes Scabiei ◽  
Effective Control ◽  
Life Stages ◽  
In Vitro Activity ◽  
Primary Human Fibroblast ◽  
Skin Cells ◽  
Low Toxicity

AbstractBackgroundScabies is a frequent cutaneous infection caused by the mite Sarcoptes scabiei in a large number of mammals including humans. As the resistance of S. scabiei against several chemical acaricides has been previously documented, the establishment of alternative and effective control molecules is required.ObjectivesIn this study, the potential acaricidal activity of beauvericin was assessed against different life stages of S. scabiei var. suis and, in comparison with dimpylate and ivermectin, two commercially available molecules used for the treatment of S. scabiei infection in animals and/or humans.MethodsIn our in vitro model, developmental stages of S. scabiei have been placed in Petri dishes filled with Columbia agar supplemented with pig serum and different concentrations of the drugs. Moreover, the toxicity of beauvericin against cultured human fibroblast skin cells was evaluated using an MTT proliferation assayResultsBeauvericin showed higher activity against adults and eggs of S. scabiei when compared to dimpylate and ivermectin. In addition, cell sensitivity assays demonstrated low toxicity of beauvericin against primary human fibroblast skin cells.ConclusionThese results revealed that the use of beauvericin is promising and might be considered for the treatment of S. scabiei infection.

scholarly journals In Vitro Activity of Beauvericin against All Developmental Stages of Sarcoptes scabiei

2020 ◽  
Vol 64 (5) ◽  
Author(s):  
Charbel Al Khoury ◽  
Nabil Nemer ◽  
Georges Nemer ◽  
Mazen Kurban ◽  
Charlotte Bernigaud ◽  
...  
Keyword(s):  
Human Fibroblast ◽  
Developmental Stages ◽  
Sarcoptes Scabiei ◽  
Effective Control ◽  
Life Stages ◽  
Primary Human Fibroblast ◽  
Content Type ◽  
Skin Cells ◽  
Low Toxicity

ABSTRACT Scabies is a frequent cutaneous infection caused by the mite Sarcoptes scabiei in a large number of mammals, including humans. As the resistance of S. scabiei against several chemical acaricides has been previously documented, the establishment of alternative and effective control molecules is required. In this study, the potential acaricidal activity of beauvericin was assessed against different life stages of S. scabiei var. suis and in comparison with dimpylate and ivermectin, two commercially available molecules used for the treatment of S. scabiei infection in animals and/or humans. The toxicity of beauvericin against cultured human fibroblast skin cells was evaluated using an MTT proliferation assay. In our in vitro model, developmental stages of S. scabiei were placed in petri dishes filled with Columbia agar supplemented with pig serum and different concentrations of the drugs. Cell sensitivity assays demonstrated low toxicity of beauvericin against primary human fibroblast skin cells. At 0.5 and 5 mM, beauvericin showed higher activity against adults and eggs of S. scabiei compared to dimpylate and ivermectin. These results revealed that the use of beauvericin is promising and might be considered for the treatment of S. scabiei infection.

scholarly journals In vitro activity of ten essential oils against Sarcoptes scabiei

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Fang Fang ◽  
Kerdalidec Candy ◽  
Elise Melloul ◽  
Charlotte Bernigaud ◽  
Ling Chai ◽  
...  
Keyword(s):  
Essential Oils ◽  
Sarcoptes Scabiei ◽  
Vitro Activity ◽  
In Vitro Activity

scholarly journals In vitro activity of usnic acid potassium salt against different developmental stages of Schistosoma mansoni: An ultrastructural study

Acta Tropica ◽  
2020 ◽  
Vol 201 ◽  
pp. 105159
Author(s):  
Hallysson D.A. Araújo ◽  
Victor H.B. Santos ◽  
Fábio A. Brayner ◽  
Luiz C. Alves ◽  
Nicácio H. Silva ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Ultrastructural Study ◽  
Potassium Salt ◽  
Developmental Stages ◽  
Usnic Acid ◽  
Vitro Activity ◽  
In Vitro Activity

Searching for new drugs for Chagas diseases: triazole analogs display high in vitro activity against Trypanosoma cruzi and low toxicity toward mammalian cells

2018 ◽  
Vol 50 (2) ◽  
pp. 81-91 ◽  
Author(s):  
Robson Xavier Faria ◽  
Daniel Tadeu Gomes Gonzaga ◽  
Paulo Anastácio Furtado Pacheco ◽  
André Luis Almeida Souza ◽  
Vitor Francisco Ferreira ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Mammalian Cells ◽  
New Drugs ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Low Toxicity

scholarly journals Composition and acaricidal activity of essential oil from Elsholtzia densa Benth against Sarcoptes scabiei mites in vitro

2019 ◽  
Vol 64 (No. 4) ◽  
pp. 178-183 ◽  
Author(s):  
Y Zhou ◽  
F Liao ◽  
J Weng ◽  
Q Mo ◽  
R Xu ◽  
...  
Keyword(s):  
Essential Oil ◽  
Liquid Paraffin ◽  
Volatile Oil ◽  
Sarcoptes Scabiei ◽  
Acaricidal Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Effective Control ◽  
In Vitro Toxicity ◽  
Ms Analysis

Plant-based natural products represent an alternative to chemical compounds for the control of mites in veterinary medicine. Here, the essential oil of Elsholtzia densa (E. densa) Benth was extracted using hydrodistillation at a rate of 1.2%. The chemical composition of the essential oil was determined by gas chromatography-mass spectrometry (GC-MS) analysis. The GC-MS analysis indicated that the principal compounds in the volatile oil of the sample were 4-Pyridinol (28.16%) and thymol (26.58%). The acaricidal activity of E. densa oil against Sarcoptes scabiei (S. scabiei) was tested in vitro. Toxicity test data were analysed using a complementary log-log (CLL) model. The E. densa oil was prepared in five concentrations by dilution with liquid paraffin (1, 2, 4, 8 and 16 mg/ml) and exhibited strong toxicity against S. scabiei with LT<sub>50</sub> values of 16.637, 5.075, 2.884, 1.184 and 0.760 h, respectively. The LC<sub>50</sub> values were 7.678, 4.623, 2.543, 1.502, 1.298 and 0.981 mg/ml for S. scabiei at 1, 2, 4, 8, 16 and 24 h, respectively. Compared to the control, the essential oil showed significant effects against S. scabiei in vitro. At 16 mg/ml, E. densa oil was found to kill all mites within a 16-h period. The results indicate that E. densa oil possesses potential acaricidal activity in vitro and may be exploited as a novel drug for the effective control of S. scabiei.

Synthesis and Antitrypanosomal Profile of Novel Hydrazonoyl Derivatives

2020 ◽  
Vol 16 (4) ◽  
pp. 487-494
Author(s):  
Natália N. Santiago ◽  
Giulianna P. de Alcântara ◽  
Juliana S. da Costa ◽  
Samir A. Carvalho ◽  
Juliana M.C. Barbosa ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Mammalian Cells ◽  
In Vitro Activity ◽  
Potential Toxicity ◽  
Standard Drug ◽  
New Family ◽  
The World ◽  
Low Toxicity

Background: Approximately, 5-7 million people are infected with T. cruzi in the world, and approximately 10,000 people per year die of complications linked to this disease. Method: This work describes the construction of a new family of hidrazonoyl substituted derivatives, structurally designed exploring the molecular hybridization between megazol and nitrofurazone. Results and Discussion: The compounds were evaluated for their in vitro activity against bloodstream trypomastigotes of Trypanosoma cruzi, etiological agent of Chagas disease, and for their potential toxicity to mammalian cells. Conclusion: Among these hydrazonoyl derivatives, we identified the derivative (4) that showed trypanocidal activity (IC50/24 h = 15.0 µM) similar to Bz, the standard drug, and low toxicity to mammalian cells, reaching an SI value of 18.7.

Correlated Studies of Cellular Interactions Using TEM, Freeze Etching, and SEM

1974 ◽  
Vol 32 ◽  
pp. 320-321
Author(s):  
J. P. Revel
Keyword(s):  
Developmental Stages ◽  
Three Dimensional ◽  
Cell Movement ◽  
Cellular Interactions ◽  
Serial Sections ◽  
Cell Sheets ◽  
Freeze Etching ◽  
Early Developmental

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

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