scholarly journals Diagnosis of Centrocestus formosanus Infection in Zebrafish (Danio rerio): A Window on a New Globalization-Derived Invasive Parasite

2019 ◽  
Author(s):  
Antonino Pace ◽  
Ludovico Dipineto ◽  
Serena Aceto ◽  
Maria Concetta Censullo ◽  
Maria Carmen Valoroso ◽  
...  
Keyword(s):  
Invasive Species ◽  
Monitoring Program ◽  
Gastrointestinal Symptoms ◽  
Control Measures ◽  
Complex Life Cycle ◽  
Digenetic Trematode ◽  
Specific Primers ◽  
Vertebrate Hosts ◽  
Species Specific ◽  
Fish Industry

AbstractCentrocestus formosanusis a digenetic trematode with a complex life cycle, involving invertebrate and vertebrate hosts, humans included. In particular, it causes gill lesions and mortality in freshwater fish species, and gastrointestinal symptoms in infected humans. Here,we describe the occurrence of C. formosanus infection in zebrafish imported in Italy. Gill arches of 30 zebrafish were examined for the presence of encysted metacercariae under a stereomicroscope, and processed through molecular analyses targeting the ribosomal Internal Transcribed Sequence 2 (ITS2) using species-specific primers of C. formosanus. Encysted metacercariae were found on the gills of 20/30 zebrafish and all were identified as C. formosanus.Despite C. formosanus distribution was originally restricted to Asia, it has been subsequently reported in new countries, revealing itself as an invasive species, and raising important concerns for biodiversity, economy, animal and public health. Given the crucial role likely played by the ornamental fish industry in the spreading of this parasite, there is an urgent need for control measures to prevent the introduction and establishment of C. formosanus in non-endemic areas, Europe included. An adequate surveillance and health-monitoring program should be conducted in the development of microbiological and epidemiological approaches to diagnose and face these new globalization-derived invasive species.

scholarly journals Diagnosis of Centrocestus formosanus Infection in Zebrafish (Danio rerio) in Italy: A Window to a New Globalization-Derived Invasive Microorganism

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 456
Author(s):  
Antonino Pace ◽  
Ludovico Dipineto ◽  
Serena Aceto ◽  
Maria Concetta Censullo ◽  
Maria Carmen Valoroso ◽  
...  
Keyword(s):  
Invasive Species ◽  
Gastrointestinal Symptoms ◽  
Control Measures ◽  
Complex Life Cycle ◽  
Digenetic Trematode ◽  
Vertebrate Hosts ◽  
Species Specific ◽  
Fish Industry ◽  
New Strategies ◽  
Diagnostic Investigations

Centrocestus formosanus is a digenetic trematode with a complex life cycle, involving invertebrate and vertebrate hosts, humans included. In particular, it causes gill lesions and mortality in freshwater fish species, and gastrointestinal symptoms in infected humans. Here, we describe the occurrence of C. formosanus infection in zebrafish imported in Italy and propose a newly designed species-specific primer pair to ameliorate the diagnostic investigations for C. formosanus. Gill arches of 30 zebrafish were examined for the presence of encysted metacercariae under a stereomicroscope and processed through molecular analyses targeting the ribosomal internal transcribed sequence 2 (ITS2). Although C. formosanus distribution was originally restricted to Asia, it has been subsequently reported in new countries, revealing itself as an invasive species and raising important concerns for biodiversity, economy, scientific research, as well as animal and public health. Given the crucial role played by the ornamental fish industry in spreading this parasite, there is an urgent need for control measures to prevent the introduction and establishment of C. formosanus in non-endemic areas, including Europe. We also suggest developing new strategies in microbiology and epidemiology to better explore this new globalization-derived invasive species.

scholarly journals Environmental DNA and Specific Primers for Detecting the Invasive Species Ectopleura crocea (Hydrozoa: Anthoathecata) in Seawater Samples

2020 ◽  
Vol 12 (6) ◽  
pp. 2360 ◽  
Author(s):  
Philjae Kim ◽  
Tae Joong Yoon ◽  
Sook Shin
Keyword(s):  
Invasive Species ◽  
Survey Methods ◽  
Environmental Dna ◽  
Visual Assessment ◽  
Molecular Techniques ◽  
Mitochondrial Cytochrome ◽  
Specific Primer ◽  
Specific Primers ◽  
Seawater Samples ◽  
Species Specific

In marine environments, environmental DNA (eDNA) can be effectively detected and possibly quantified when combined with molecular techniques, as demonstrated by several recent studies. In this study, we developed a species-specific primer set and a probe to detect the distribution and biomass of an invasive hydrozoan in South Korea, Ectopleura crocea. These molecular markers were designed to amplify a 187 bp region based on mitochondrial cytochrome c oxidase subunit I (COI) of E. crocea and were tested on seawater samples from 35 Korean harbors in 2017. Of the 35 sites we investigated, only nine harbors returned positive detections when using traditional survey methods, while surveys based on the use of eDNA techniques detected E. crocea DNA in all seawater samples. These results suggest that eDNA surveys based on molecular techniques are more effective at identifying species distribution and estimating biomass than traditional surveys based on visual assessment of morphology.

Species-specific duplex PCR for the detection of Pratylenchus penetrans

Nematology ◽  
2009 ◽  
Vol 11 (6) ◽  
pp. 847-857 ◽  
Author(s):  
Lieven Waeyenberge ◽  
Nicole Viaene ◽  
Maurice Moens
Keyword(s):  
Internal Control ◽  
Dna Sequences ◽  
Rrna Gene ◽  
Duplex Pcr ◽  
Specific Primers ◽  
5.8S Rrna Gene ◽  
5.8S Rrna ◽  
Wide Range ◽  
Pcr Products ◽  
Species Specific

Abstract ITS1, the 5.8S rRNA gene and ITS2 of the rDNA region were sequenced from 20 different Pratylenchus species. Additionally, the same region was sequenced from seven populations of P. penetrans. After purifying, cloning and sequencing the PCR products, all sequences were aligned in order to find unique sites suitable for the design of species-specific primers for P. penetrans. Since ITS regions showed variability between and even within populations of P. penetrans, only three small DNA sequences were suitable for the construction of three potentially useful species-specific primers. New species-specific primers were paired with existing universal ITS primers and tested in all possible primer combinations. The best performing primer set, supplemented with a universal 28S rDNA primer set that served as an internal control, was tested in duplex PCR. The ideal annealing temperature, Mg2+ concentration and primer ratios were then determined for the most promising primer set. The optimised duplex PCR was subsequently tested on a wide range of different Pratylenchus spp. and 25 P. penetrans populations originating from all over the world. To test the sensitivity, the duplex PCR was conducted on DNA extracted from a single P. penetrans nematode mixed with varying amounts of nematodes belonging to another Pratylenchus species. Results showed that a reliable and sensitive P. penetrans species-specific duplex PCR was constructed.

Species‐specific primers of chitin synthase 1 gene for the differentiation of the Trichophyton mentagrophytes complex

Mycoses ◽  
1999 ◽  
Vol 42 (1‐2) ◽  
pp. 71-74 ◽  
Author(s):  
R. Kano ◽  
Y. Nakamura ◽  
T. Watari ◽  
S. Watanabe ◽  
H. Takahashi ◽  
...  
Keyword(s):  
Trichophyton Mentagrophytes ◽  
Chitin Synthase ◽  
Specific Primers ◽  
Trichophyton Mentagrophytes Complex ◽  
Species Specific
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