scholarly journals The consequences of surviving infection across the metamorphic boundary: tradeoff insights from RNAseq and life history measures

2019 ◽  
Author(s):  
Naomi L.P. Keehnen ◽  
Lucie Kučerová ◽  
Sören Nylin ◽  
Ulrich Theopold ◽  
Christopher W. Wheat
Keyword(s):  
Life History ◽  
Micrococcus Luteus ◽  
Larval Instar ◽  
Developmental Rate ◽  
Pupal Weight ◽  
Negative Consequences ◽  
Critical Periods ◽  
Long Term Effects ◽  
Strong Suppression ◽  
The Impact

AbstractThe broad diversity of insect life has been shaped, in part, by pathogen pressure, yet the influence of injury and infection during critical periods of development is understudied. During development, insects undergo metamorphosis, wherein the organism experiences a dramatic shift in their overall morphology, and physiology. In temperate zones, metamorphosis is often directly followed by a developmental arrest called diapause, for which the insect needs to acquire enough energy reserves before the onset of winter. We investigated the long-term effects of injury and infection using two bacteria in the butterfly Pieris napi, revealing that the negative consequences of bacterial infection carry across the metamorphic boundary. Initial direct effects of infection were weight loss and slower development, as well as an increased mortality at higher infection levels. The detrimental effects were stronger in the gram-positive Micrococcus luteus compared to gram-negative Escherichia coli. Transcriptome-wide differences between the two bacteria were already observed in the gene expression profile of the first 24 hours after infection. Larvae infected with M. luteus showed a strong suppression of all non-immunity related processes, with several types of immune responses being activated. The impact of these transcriptomic changes, a tradeoff between homeostasis and immune response, were visible in the life history data, wherein individuals infected with M. luteus had the highest mortality rate, along with the lowest pupal weight, developmental rate and adult weight of all the treatments. Overall, we find that the cost of infection and wounding in the final larval instar carries over the metamorphic boundary, and is expected to negatively affect their lifetime fitness.

scholarly journals Transient inhibition and long-term facilitation of locomotion by phasic optogenetic activation of serotonin neurons

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Patrícia A Correia ◽  
Eran Lottem ◽  
Dhruba Banerjee ◽  
Ana S Machado ◽  
Megan R Carey ◽  
...  
Keyword(s):  
Motor Behavior ◽  
Motor Impairment ◽  
Long Term Effects ◽  
Strong Suppression ◽  
Serotonin Neurons ◽  
Short And Long Term ◽  
Rapid Kinetics ◽  
Long Term Facilitation ◽  
The Impact

Serotonin (5-HT) is associated with mood and motivation but the function of endogenous 5-HT remains controversial. Here, we studied the impact of phasic optogenetic activation of 5-HT neurons in mice over time scales from seconds to weeks. We found that activating dorsal raphe nucleus (DRN) 5-HT neurons induced a strong suppression of spontaneous locomotor behavior in the open field with rapid kinetics (onset ≤1 s). Inhibition of locomotion was independent of measures of anxiety or motor impairment and could be overcome by strong motivational drive. Repetitive place-contingent pairing of activation caused neither place preference nor aversion. However, repeated 15 min daily stimulation caused a persistent increase in spontaneous locomotion to emerge over three weeks. These results show that 5-HT transients have strong and opposing short and long-term effects on motor behavior that appear to arise from effects on the underlying factors that motivate actions.

scholarly journals Does early vitamin B12supplementation improve neurodevelopment and cognitive function in childhood and into school age: a study protocol for extended follow-ups from randomised controlled trials in India and Tanzania

BMJ Open ◽  
2018 ◽  
Vol 8 (2) ◽  
pp. e018962 ◽  
Author(s):  
Brita Askeland Winje ◽  
Ingrid Kvestad ◽  
Srinivasan Krishnamachari ◽  
Karim Manji ◽  
Sunita Taneja ◽  
...  
Keyword(s):  
Cognitive Function ◽  
Controlled Trials ◽  
Vitamin B ◽  
Critical Periods ◽  
Long Term Effects ◽  
Oral Vitamin ◽  
Neurodevelopmental Outcomes ◽  
Developmental Potential ◽  
Link Type ◽  
The Impact

IntroductionAs many as 250 million children under the age of 5 may not be reaching their full developmental potential partly due to poor nutrition during pregnancy and the first 2 years of life. Micronutrients, including vitamin B12, are important for the development of brain structure and function; however, the timing, duration and severity of deficiencies may alter the impact on functional development outcomes. Consequently, to fully explore the effect of vitamin B12on cognitive function, it is crucial to measure neurodevelopment at different ages, in different populations and with vitamin B12supplementation at different times during the critical periods of neurodevelopment.Methods and analysisIn this project, we follow up children from four recently completed randomised placebo-controlled trials of oral vitamin B12supplementation, two in India and two in Tanzania, to explore the long-term effects on neurodevelopmental outcomes and growth. All the included trials provided at least two recommended dietary allowances of oral vitamin B12daily for at least 6 months. Vitamin B12was supplemented either during pregnancy, early infancy or early childhood. Primary outcomes are neurodevelopmental status, cognitive function and growth later in childhood. We apply validated and culturally appropriate instruments to identify relevant developmental outcomes. All statistical analyses will be done according to intention-to-treat principles. The project provides an excellent opportunity to examine the effect of vitamin B12supplementation in different periods during early life and measure the outcomes later in childhood.Ethics and disseminationThe study has received ethical approvals from all relevant authorities in Norway, USA, Tanzania and India and complies fully with ethical principles for medical research. Results will be presented at national and international research and policy meetings and published in peer-reviewed scientific journals, preferably open access.Trial registration numberNCT00641862 (Bangalore);NCT00717730, updated CTRI/2016/11/007494 (Delhi);NCT00197548andNCT00421668(Dar es Salaam).

scholarly journals Physiological Tradeoffs of Immune Response Differs by Infection Type in Pieris napi

2021 ◽  
Vol 11 ◽  
Author(s):  
Naomi L. P. Keehnen ◽  
Lucie Kučerová ◽  
Sören Nylin ◽  
Ulrich Theopold ◽  
Christopher W. Wheat
Keyword(s):  
Immune Response ◽  
Life History ◽  
Immune Responses ◽  
Negative Impact ◽  
Micrococcus Luteus ◽  
Infection Type ◽  
Later Life ◽  
Pupal Weight ◽  
Pieris Napi ◽  
Successful Infection

Understanding the tradeoffs that result from successful infection responses is central to understanding how life histories evolve. Gaining such insights, however, can be challenging, as they may be pathogen specific and confounded with experimental design. Here, we investigated whether infection from gram positive or negative bacteria results in different physiological tradeoffs, and whether these infections impact life history later in life (post-diapause development), in the butterfly Pieris napi. During the first 24 h after infection (3, 6, 12, and 24 h), after removing effects due to injection, larvae infected with Micrococcus luteus showed a strong suppression of all non-immunity related processes while several types of immune responses were upregulated. In contrast, this tradeoff between homeostasis and immune response was much less pronounced in Escherichia coli infections. These differences were also visible long after infection, via weight loss and slower development, as well as an increased mortality at higher infection levels during later stages of development. Individuals infected with M. luteus, compared to E. coli, had a higher mortality rate, and a lower pupal weight, developmental rate and adult weight. Further, males exhibited a more negative impact of infection than females. Thus, immune responses come at a cost even when the initial infection has been overcome, and these costs are likely to affect later life history parameters with fitness consequences.

Long-term consequences of early nutritional conditions on the behaviour and growth of fire salamanders

2016 ◽  
Vol 37 (1) ◽  
pp. 69-77 ◽  
Author(s):  
E. Tobias Krause ◽  
Barbara A. Caspers
Keyword(s):  
Life History ◽  
Later Life ◽  
Exploratory Behaviour ◽  
Future Research ◽  
Developmental Stress ◽  
Long Term Effects ◽  
Sibling Pairs ◽  
Nutritional Conditions ◽  
The Impact

Early developmental conditions can have a strong influence on the life history. Fire salamanders represent an interesting system due to their biphasic life cycle consisting of an aquatic larval stage and a subsequent terrestrial stage. The environment experienced as larvae affects growth, age at metamorphosis and behaviour. In particular, diet restrictions can cause developmental stress. However, the impact of such developmental stress on the growth and behaviour in a long-lived amphibian are not yet well studied. We manipulated the early diet of sibling pairs of fire salamander larvae. One sibling was raised under food-restriction and the other was raised under conditions of abundant food. We report long-term effects on growth and exploratory behaviour; exploratory behaviour was tested in two sessions, first as young juveniles and later as sub-adults. We found that food-restricted conditions during early development affected exploratory behaviour. Early deficits in body mass and body size were compensated later in life, by approximately 18 months of age. When morphological differences were compensated, the initial differences in exploratory behaviour also disappeared. Thus, compensation not only led to an alignment of body parameters but was also accompanied by an adjustment in exploratory behaviour. No cost of compensation was detected, but future research will need to verify whether the potential costs of compensation are negligible in slow-growing salamanders or whether they are shifted to later life stages. Our study provides novel insights into the life history of fire salamanders and reveals that early larval conditions have effects on individuals long after metamorphosis.

SUMBER-SUMBER RESILIENSI PADA REMAJA AKHIR YANG MENGALAMI KEKERASAN DARI ORANGTUA PADA MASA KANAK-KANAK

Psibernetika ◽  
2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Devina Calista ◽  
Garvin Garvin
Keyword(s):  
Late Adolescents ◽  
Long Term Effects ◽  
Short Term ◽  
Parental Affection ◽  
Resilience Factors ◽  
Childhood Violence ◽  
Past Experiences ◽  
The Impact ◽  
Depth Interviews

<p><em>Child abuse by parents is common in households. The impact of violence on children will bring short-term effects and long-term effects that can be attributed to their various emotional, behavioral and social problems in the future; especially in late adolescence that will enter adulthood. Resilience factors increase the likelihood that adolescents who are victims of childhood violence recover from their past experiences</em><em>,</em><em> become more powerful individuals and have a better life. The purpose of this study was to determine the source of resilience in late adolescents who experienced violence from parents in their childhood. This research uses qualitative research methods with in-depth interviews as a method of data collection. The result shows that the three research participants have the aspects of "I Have", "I Am", and "I Can"; a participant has "I Can" aspects as a source of resilience, and one other subject has no source of resilience. The study concluded that parental affection and acceptance of the past experience have role to the three sources of resilience (I Have, I Am, and I Can)</em></p><p><em> </em></p><p><strong><em>Keyword : </em></strong><em>Resilience, adolescence, violence, parents</em></p>

Conflicts in Biafra, Katanga and Cabinda as a result of the geopolitical legacy of colonialism

2020 ◽  
Vol 2020 (10-3) ◽  
pp. 238-246
Author(s):  
Olga Dzhenchakova
Keyword(s):  
Democratic Republic ◽  
Economic Factor ◽  
Colonial Period ◽  
Sub Saharan Africa ◽  
Negative Consequences ◽  
Post Colonial ◽  
Republic Of The Congo ◽  
Sub Saharan ◽  
The Republic ◽  
The Impact

The article considers the impact of the colonial past of some countries in sub-Saharan Africa and its effect on their development during the post-colonial period. The negative consequences of the geopolitical legacy of colonialism are shown on the example of three countries: Nigeria, the Democratic Republic of the Congo and the Republic of Angola, expressed in the emergence of conflicts in these countries based on ethno-cultural, religious and socio-economic contradictions. At the same time, the focus is made on the economic factor and the consequences of the consumer policy of the former metropolises pursuing their mercantile interests were mixed.

HOW CLIMATE CHANGE CAN INFLUENCE THE AGRICULTURE IN UKRAINE: A REVIEW

2020 ◽  
Author(s):  
N. Maidanovych ◽  
Keyword(s):  
Climate Change ◽  
Crop Yields ◽  
Soil Surface ◽  
Winter Period ◽  
Negative Consequences ◽  
Resource Saving ◽  
Impact Of Climate Change ◽  
Positive Effects ◽  
Average Annual Temperature ◽  
The Impact

The purpose of this work is to review and analyze the main results of modern research on the impact of climate change on the agro-sphere of Ukraine. Results. Analysis of research has shown that the effects of climate change on the agro-sphere are already being felt today and will continue in the future. The observed climate changes in recent decades have already significantly affected the shift in the northern direction of all agro-climatic zones of Europe, including Ukraine. From the point of view of productivity of the agro-sphere of Ukraine, climate change will have both positive and negative consequences. The positives include: improving the conditions of formation and reducing the harvesting time of crop yields; the possibility of effective introduction of late varieties (hybrids), which require more thermal resources; improving the conditions for overwintering crops; increase the efficiency of fertilizer application. Model estimates of the impact of climate change on wheat yields in Ukraine mainly indicate the positive effects of global warming on yields in the medium term, but with an increase in the average annual temperature by 2 ° C above normal, grain yields are expected to decrease. The negative consequences of the impact of climate change on the agrosphere include: increased drought during the growing season; acceleration of humus decomposition in soils; deterioration of soil moisture in the southern regions; deterioration of grain quality and failure to ensure full vernalization of grain; increase in the number of pests, the spread of pathogens of plants and weeds due to favorable conditions for their overwintering; increase in wind and water erosion of the soil caused by an increase in droughts and extreme rainfall; increasing risks of freezing of winter crops due to lack of stable snow cover. Conclusions. Resource-saving agricultural technologies are of particular importance in the context of climate change. They include technologies such as no-till, strip-till, ridge-till, which make it possible to partially store and accumulate mulch on the soil surface, reduce the speed of the surface layer of air and contribute to better preservation of moisture accumulated during the autumn-winter period. And in determining the most effective ways and mechanisms to reduce weather risks for Ukrainian farmers, it is necessary to take into account the world practice of climate-smart technologies.

Financial Risks of the Bank: Assessment and Mechanism of Neutralization

2020 ◽  
Vol 22 (1) ◽  
pp. 6-12
Author(s):  
Nelia Volkova ◽  
◽  
Alina Mukhina ◽  
Keyword(s):  
Risk Assessment ◽  
Financial Stability ◽  
Financial Risk ◽  
Stable Operation ◽  
Financial Risks ◽  
Negative Consequences ◽  
Conceptual Approach ◽  
Relevant Today ◽  
Banking Institution ◽  
The Impact

Abstract. Introduction. The issue of financial risk management of commercial banks is quite relevant today, because the activity of banks is the most risky of all. The presence of risks in banking can lead to unexpected losses, namely the loss of own resources. That’s why for the stable operation of the bank without loss the priority is to assess the financial risks, which is the basis for their further neutralization. Purpose. The purpose of the article is to develop conceptual provisions for assessment financial risks and justifying the need to neutralize them. Results. The article analyzes the impact of risks on the financial stability of a banking institution. The main methods of bank risk assessment are considered. All these include the statistical method, the analytical method, the expert method, the analogue method and the combined method. The necessity of neutralization of financial risks in order to avoid negative consequences is substantiated. Also the methods of bank risks neutralization are considered. It should be noted that these methods of neutralization can not only be used, but also supplement the list with new methods must be done, which in the future will protect the bank from the influence of undesirable factors. A conceptual approach to the assessment and neutralization of financial risks is proposed. This conceptual approach aims to ensure effective assessment of the level of risk with their subsequent neutralization Conclusions. Use of a conceptual approach will allow an effective risk assessment and decision-making to avoid or accept risk. Thanks to using this approach, the banking institution will be able to react swiftly to the presence of financial risks and to prevent the occurrence of negative consequences, which may lead to a violation of the financial stability of the bank.

The Impact of Sanctions on North Korea’s Foreign Trade from the Perspective of the Comparative Advantage Theory

2019 ◽  
pp. 4-15
Author(s):  
I. A. Korgun ◽  
G. D. Toloraya
Keyword(s):  
Foreign Trade ◽  
Competitive Advantage ◽  
National Economy ◽  
North Korea ◽  
Rent Seeking ◽  
Economic Transformation ◽  
Global Trade ◽  
Negative Consequences ◽  
The North ◽  
The Impact

The presented study analyzes the opportunities for North Korea to capitalize on its competitive advantages in foreign trade in the context of sanctions.Aim. The study aims to identify mechanisms that allow North Korea to engage in foreign trade in circumvention of UN sanctions and to analyze their impact on the national economy.Tasks. The authors analyze the structure of North Korea’s national economy, its initial competitive advantage, identify the specific features of North Korea’s foreign trade in the context of sanctions, and determine the consequences of illicit trade in circumvention of sanctions for the national economy.Methods. This study uses an interdisciplinary approach that combines the classical theory of competitive advantage with the concept of rent seeking, with the concept of rent seeking and analysis of trade flows.Results. The study shows that, despite the restrictions imposed by sanctions, North Korea strives to make the most of its advantages, such as resource availability and cheap labor, in global trade. The country builds its own export-import chains in circumvention of sanctions. These chains are rather mobile, flexible, and controlled by the elite. As a result, benefits from trade that could be evenly distributed among the population are concentrated in the hands of a narrow segment of society. ‘Rent seeking’ makes it possible to formulate the negative consequences of these processes for the North Korean economy and the international community.Conclusions. Solving the North Korean issue requires an economic transformation in the country through the replacement of restrictive sanctions with more constructive ones. The exclusion of North Korea from open global trade leads to the country’s marginalization and impairs the transparency of international commodity flows.

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