scholarly journals Organic soil amendment with Spent Mushroom Substrate results in fungal colonisation, alters bacterial-fungal co-occurrence patterns and improves plant productivity

2019 ◽  
Author(s):  
Fabiana S. Paula ◽  
Enrico Tatti ◽  
Camilla Thorn ◽  
Florence Abram ◽  
Jude Wilson ◽  
...  
Keyword(s):  
Organic Matter ◽  
Community Structure ◽  
Soil Amendment ◽  
Organic Matter Content ◽  
Plant Productivity ◽  
Fungal Communities ◽  
Spent Mushroom Substrate ◽  
Soil System ◽  
Occurrence Patterns ◽  
Complex Organic

AbstractIn agricultural systems based on organic fertilisers, the activity of prokaryotes and fungi is essential for degradation of complex substrates and release of nutrients for plant uptake. Understanding the dynamics of microbial communities in these systems is, therefore, desirable for designing successful management strategies aiming to optimise nutrient availability and to improve plant productivity. Of particular interest is how the microbial inoculum provided by an organic substrate persists in the soil and interacts with soil and plant microbiomes, as these processes may affect the long-term benefits of organic amendments. We aimed to investigate how these dynamics occurred in soil treated with stabilised spent mushroom substrate (SMS), a soil amendment rich in nutrients and complex organic matter. We carried out a 14 weeks soil trial to assess the plant growth promoting properties of the SMS and to monitor the successional processes of the resulting SMS-soil communities compared to a mineral amended control. Bacterial and fungal communities were analysed by high-throughput sequencing at both DNA and RNA (cDNA) levels. Using a combination of computational tools, including SourceTracker and Network analysis, we assessed the persistence of SMS-derived taxa in soil, and the changes in co-occurrence patterns and microbial community structure over time. Prokaryotic and fungal communities presented remarkably distinct trajectories following SMS treatment. The soil prokaryotic communities displayed higher levels of resilience to the changes introduced by SMS treatment and rapidly tended toward a soil-like profile, with low persistence of SMS-derived prokaryotes. In contrast, the SMS fungal community had greater success in soil colonisation during the time monitored. SMS treatment promoted an increase in the participation of fungi in the highly connected fraction of the active community, including fungal taxa of SMS origin. We observed the presence of highly connected microbial guilds, composed by fungal and bacterial taxa with reported capabilities of complex organic matter degradation. Many of these taxa were also significantly correlated with the organic matter content and plant yield, suggesting that these highly connected taxa may play key roles not only in the community structure, but also in the plant-soil system under organic fertilisation.

DNA Sequence and Community Structure Diversity of Multi-Year Soil Fungi in Grape of Xinjiang

2021 ◽  
Author(s):  
Tong Liu ◽  
Feng Xue
Keyword(s):  
Organic Matter ◽  
Community Structure ◽  
Soil Organic Matter ◽  
Soil Fungi ◽  
Rhizosphere Soil ◽  
Middle Layer ◽  
Fungal Communities ◽  
Seedless Grape ◽  
Significant Difference ◽  
Vineyard Soil

Abstract This study is designed to understand the community structure and diversity of fungi in the rhizosphere soil of grape. As the sample for this study, the rhizosphere soil of Crimson seedless grape with different planting years was collected from Shihezi in Xinjiang to carry out high-throughput sequencing, by which the complete sequence of soil fungi DNA was identified, and accordingly, the richness and diversity index of fungi were determined. The results showed that the dominant phyla of fungi in the grape rhizosphere soil with different planting years were Ascomycota and Basidiomycota, and the dominant classes of fungi were Sordariomycetes and Dothideomycetes. Soil organic matter, total potassium, total nitrogen and available phosphorus were the main soil fertility factors affecting the abundance and diversity of soil fungal communities, among which soil organic matter had the most significant influence. In addition, the fungal diversity and richness were highest in the middle layer (20-35 cm) of the grape rhizosphere soil with 12 planting years and lowest in the lower layer (35-50 cm) of the grape rhizosphere soil with 5 planting years. Linear discriminant analysis suggested that there were more biomarkers in the vineyard rhizosphere soil with 10 planting years, which meant there were more fungal communities with significant difference in the soil, especially in the middle layer (20-35). The results of this study can provide data reference and theoretical basis for improving vineyard soil quality, evaluating soil microecological effects and improving ecological environment of vineyard soil.

scholarly journals Radionuclides and heavy metal contents in phosphogypsum samples in comparison to cerrado soils

2009 ◽  
Vol 33 (5) ◽  
pp. 1481-1488 ◽  
Author(s):  
Vanusa Maria Feliciano Jacomino ◽  
Kerley Alberto Pereira de Oliveira ◽  
Maria Helena Tirollo Taddei ◽  
Maria Célia Siqueira ◽  
Maria Eleonora Deschamps Pires Carneiro ◽  
...  
Keyword(s):  
Organic Matter ◽  
Solid Waste ◽  
Soil Amendment ◽  
Natural Radionuclides ◽  
Organic Matter Content ◽  
Maximum Level ◽  
Mineralogical Composition ◽  
Matter Content ◽  
Soil Samples ◽  
226Ra Activity

Phosphogysum (PG) or agricultural gypsum, a solid waste from the phosphate fertilizer industry, is used as soil amendment, especially on soils in the Cerrado region, in Brazil. This material may however contain natural radionuclides and metals which can be transferred to soils, plants and water sources. This paper presents and discusses the results of physical and chemical analyses that characterized samples of PG and compares them to the results found in two typical soils of the Cerrado, a clayey and sandy one. These analyses included: solid waste classification, evaluation of organic matter content and of P, K, Ca, Mg, and Al concentrations and of the mineralogical composition. Natural radionuclides and metal concentrations in PG and soil samples were also measured. Phosphogypsum was classified as Class II A - Not Dangerous, Not Inert, Not Corrosive and Not Reactive. The organic matter content in the soil samples was low and potential acidity high. In the mean, the specific 226Ra activity in the phosphogypsum samples (252 Bq kg-1) was below the maximum level recommended by USEPA, which is 370 Bq kg-1 for agricultural use. In addition, this study verified that natural radionuclides and metals concentrations in PG were lower than in the clayey Oxisol of Sete Lagoas, Minas Gerais, Brazil. These results indicated that the application of phosphogypsum as soil amendment in agriculture would not cause a significant impact on the environment.

scholarly journals Variability of macrofauna distribution along a dissipative log-spiral sandy beach in Rio de Janeiro, Southeastern Brazil

2017 ◽  
Vol 81 (1) ◽  
pp. 111 ◽  
Author(s):  
Carlos A.M. Barboza ◽  
Tatiana Cabrini ◽  
Gustavo Mattos ◽  
Viviane Skinner ◽  
Ricardo Cardoso
Keyword(s):  
Grain Size ◽  
Organic Matter ◽  
Community Structure ◽  
Organic Matter Content ◽  
Principal Component ◽  
Matter Content ◽  
Southeastern Brazil ◽  
Individual Species ◽  
Beach Slope ◽  
Sediment Sorting

Log-spiral beaches display defined physical gradients alongshore. However, the majority of studies focus on the variability of a single population of macrofauna species. We aimed to investigate the variation in species distribution and in community structure along ten transects on a log-spiral beach. Principal component analysis indicated a clear physical gradient alongshore. Redundancy analysis showed that the sheltered end was related to smaller particle sizes, higher organic matter content and high densities of polychaetes. The exposed end was characterized by coarser sand, lower organic matter content and a high presence of crustaceans. Model selection indicated that the “best fit” to explain the variability in the number of individuals included grain size and beach slope. Variability of the polychaete Scolelepis squamata was best explained by grain size, slope and sediment sorting. The best model for the cirolanid Excirolana armata only included sediment sorting. The physical gradient in sediment texture and the beach slope explained more than one-third of the variability in community structure. The physical variables were also correlated with the distribution of the individual species. We showed that the physical gradient on log-spiral coasts may be an important driver of macrofauna variability, even at mesoscales and in dissipative conditions.

Community Structure and Co-Occurrence Network Analysis of Bacteria and Fungi in Wheat Fields vs Fruit Orchards

2021 ◽  
Author(s):  
Xinyu Cui ◽  
Huan He ◽  
Fengxiao Zhu ◽  
Xiaobo Liu ◽  
You Ma ◽  
...  
Keyword(s):  
Community Structure ◽  
Amplicon Sequencing ◽  
Yangtze River Delta ◽  
Vital Role ◽  
Fungal Communities ◽  
Rrna Gene ◽  
Network Analyses ◽  
Planting Methods ◽  
Bacteria And Fungi ◽  
Occurrence Patterns

Abstract Soil microorganisms play a vital role in biogeochemical processes and nutrient turnover in agricultural ecosystems. However, the information on how the structure and co-occurrence patterns of microbial communities respond to the change of planting methods is still limited. In this study, a total of 34 soil samples were collected from 17 different fields of two planting types (wheat and orchards) along the Taige Canal in Yangtze River Delta. The distribution and diversity of bacterial and fungal communities in soil were determined using amplicon sequencing targeting the 16S rRNA gene and ITS gene, respectively. The dominated bacteria were Proteobacteria, Acidobacteriota, Actinobacteriota, Chloroflexi, Bacteroidota, and Firmicutes. The relative abundance of Actinobacteriota and Firmicutes was higher in the orchards, while Chloroflexi and Nitrospirota were more abundant in wheat fields. Ascomycota, Mortierellomycota, and Basidiomycota were the predominant fungi in both types of soils. The diversity of bacterial and fungal communities was greater in the wheat fields than in the orchards. The statistical analyses showed that pH was the main factor shaping the community structure. Moreover, high co-occurrence patterns of bacteria and fungi were confirmed in both wheat fields and orchards. Network analyses showed that both the wheat fields and orchards occurred modular structure, which mainly contained nodes of Acidobacteriota, Chloroflexi, Gemmatimonadota, Nitrospirota and Ascomycota. In summary, our work showed the co-occurrence network and the convergence/divergence of microbial community structure in wheat fields and orchards, giving a comprehensive understanding of the microbe-microbe interaction during planting methods changes.

scholarly journals DNA sequence and community structure diversity of multi-year soil fungi in Grape of Xinjiang

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Feng Xue ◽  
Tong Liu
Keyword(s):  
Organic Matter ◽  
Community Structure ◽  
Soil Organic Matter ◽  
Soil Fungi ◽  
Rhizosphere Soil ◽  
Middle Layer ◽  
Fungal Communities ◽  
Seedless Grape ◽  
Significant Difference ◽  
Vineyard Soil

AbstractThis study is designed to understand the community structure and diversity of fungi in the rhizosphere soil of grape. As the sample for this study, the rhizosphere soil of Crimson seedless grape with different planting years was collected from Shihezi in Xinjiang to carry out high-throughput sequencing, by which the complete sequence of soil fungi DNA was identified, and accordingly, the richness and diversity index of fungi were determined. The results showed that the dominant phyla of fungi in the grape rhizosphere soil with different planting years were Ascomycota and Basidiomycota, and the dominant classes of fungi were Sordariomycetes and Dothideomycetes. Soil organic matter, total potassium, total nitrogen and available phosphorus were the main soil fertility factors affecting the abundance and diversity of soil fungal communities, among which soil organic matter had the most significant influence. In addition, the fungal diversity and richness were highest in the middle layer (20–35 cm) of the grape rhizosphere soil with 12 planting years and lowest in the lower layer (35–50 cm) of the grape rhizosphere soil with 5 planting years. Linear discriminant analysis suggested that there were more biomarkers in the vineyard rhizosphere soil with 10 planting years, which meant there were more fungal communities with significant difference in the soil, especially in the middle layer (20–35). The results of this study can provide data reference and theoretical basis for improving vineyard soil quality, evaluating soil microecological effects and improving ecological environment of vineyard soil.

Effects of long term application of chicken manure and spent mushroom substrate on organic matter and storage of water in sandy soils

2020 ◽  
Author(s):  
Jerzy Lipiec ◽  
Boguslaw Usowicz ◽  
Jerzy Klopotek ◽  
Marcin Turski ◽  
Magdalena Frac
Keyword(s):  
Organic Matter ◽  
Soil Organic Matter ◽  
Soil Quality ◽  
Organic Matter Content ◽  
Matter Content ◽  
Chicken Manure ◽  
Spent Mushroom Substrate ◽  
Soil Organic Matter Content ◽  
Water Capacity

<p>The aim of this study was to evaluate the effects of long-term application of exogenous organic matter on soil organic matter and water storage. Addition of organic matter is of importance in sandy soils that are in general poor in organic matter, acidic, conducive to drought and used in agricultural production throughout the world. In this study the sandy podzol (63-74% sand) was amended with chicken manure or waste spent mushroom substrate through more than 20 years. Soil organic matter content, water retention curves, acidity and structural stability were determined at three depths in the top 60 cm in organic amended and control plots. Enrichment of the soil with chicken manure and spent mushroom substrate caused increase in soil organic matter content in the top 0-20 cm from 1.34 to 3.50% and from 0.86 to 4.71%, respectively. Corresponding increases in field water capacity were from 13.6 to 31.8 m<sup>3</sup> m<sup>−3</sup> and from 17.7 to 27.2 m<sup>3</sup> m<sup>−3</sup>. Both amendments improved soil structure, reaction and nutrient status. In general, these positive effects were greater in chicken manure than spent mushroom substrate amended soil and less pronounced at depths 20-40 cm and 40-60 cm compared to upper soil. Increase in the field water capacity and water storage capacity made the soils amended with  organic matter more drought resistant. Our findings provide valuable insights the spent mushroom substrate left after growing the mushrooms and chicken manure are environmentally friendly and economical viable soil management practices to increase soil quality and crop productivity.</p><p> </p><p> Acknowledgements</p><p>The work was partially funded by the HORIZON 2020, European Commission, Programme: H2020-SFS-4-2014: Soil quality and function, project No. 635750, Interactive Soil Quality Assessment in Europe and China for Agricultural Productivity and Environmental Resilience (iSQAPER, 2015–2020).</p>

scholarly journals Valuable product production from wood mill effluents

2010 ◽  
Vol 62 (10) ◽  
pp. 2294-2300 ◽  
Author(s):  
T. Mato ◽  
M. Ben ◽  
C. Kennes ◽  
M. C. Veiga
Keyword(s):  
Organic Matter ◽  
Volatile Fatty Acids ◽  
Batch Reactor ◽  
Organic Matter Content ◽  
Oxygen Demand ◽  
Matter Content ◽  
Valuable Product ◽  
High Chemical Oxygen Demand ◽  
Complex Organic ◽  
Aerobic And Anaerobic

Fibreboard production is one of the most important industrial activities in Galicia (Spain). Great amounts of wastewater are generated, with properties depending on the type of wood, treatment process, final product and water reusing, among others. These effluents are characterized by a high chemical oxygen demand (COD), low pH and nutrients limitation. Aerobic and anaerobic processes have been used for their treatment. Presently, bioplastics production (mainly polyhydroxyalkanoates or PHA) from wastewaters with mixed cultures is being studied. Substrate requirements for these processes are a high organic matter content and low nutrient concentration. Therefore, wood mill effluents could be a suitable feedstock. PHA production from wastewaters is carried out in three steps. First, complex organic matter is converted into volatile fatty acids (VFA) through acidogenic fermentation. Then, VFA are used as substrate in an aerobic sequencing batch reactor (SBR), in which the enrichement of PHA producing bacteria from a mixed culture is favoured. Finally, the sludge from the SBR is fed with a pulse containing high VFA concentrations, resulting in PHA accumulation inside the cells. In this work, the possibility of applying this process to wood mill effluents is proposed. An acidification percentage of 37% and a storage yield (YSTO) of 0.23 Cmmol/Cmmol were obtained.

scholarly journals Effects of Application of Recycled Chicken Manure and Spent Mushroom Substrate on Organic Matter Acidity and Hydraulic Properties of Sandy Soils

Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 4036
Author(s):  
Jerzy Lipiec ◽  
Bogusław Usowicz ◽  
Jerzy Kłopotek ◽  
Marcin Turski ◽  
Magdalena Frąc
Keyword(s):  
Organic Matter ◽  
Soil Organic Matter ◽  
Hydraulic Conductivity ◽  
Hydraulic Properties ◽  
Organic Matter Content ◽  
Chicken Manure ◽  
Water Retention Capacity ◽  
Spent Mushroom Substrate ◽  
Resource Base ◽  
Retention Capacity

Soil organic matter is a key resource base for agriculture. However, its content in cultivated soils is low and often decreases. This study aimed at examining the effects of long-term application of chicken manure (CM) and spent mushroom substrate (SMS) on organic matter accumulation, acidity, and hydraulic properties of soil. Two podzol soils with sandy texture in Podlasie Region (Poland) were enriched with recycled CM (10 Mg ha–1) and SMS (20 Mg ha–1), respectively, every 1–2 years for 20 years. The application of CM and SMS increased soil organic matter content at the depths of 0–20, 20–40, and 40–60 cm, especially at 0–20 cm (by 102–201%). The initial soil pH increased in the CM- and SMS-amended soil by 1.7–2.0 units and 1.0–1.2 units, respectively. Soil bulk density at comparable depths increased and decreased following the addition of CM and SMS, respectively. The addition of CM increased field water capacity (at –100 hPa) in the range from 45.8 to 117.8% depending on the depth within the 0–60 cm layer. In the case of the SMS addition, the value of the parameter was in the range of 42.4–48.5% at two depths within 0–40 cm. Depending on the depth, CM reduced the content of transmission pores (>50 µm) in the range from 46.3 to 82.3% and increased the level of residual pores (<0.5 µm) by 91.0–198.6%. SMS increased the content of residual pores at the successive depths by 121.8, 251.0, and 30.3% and decreased or increased the content of transmission and storage pores. Additionally, it significantly reduced the saturated hydraulic conductivity at two depths within 0–40 cm. The fitted unsaturated hydraulic conductivity at two depths within the 0–40 cm layer increased and decreased in the CM- and SMS-amended soils, respectively. The results provide a novel insight into the application of recycled organic materials to sequester soil organic matter and improve crop productivity by increasing soil water retention capacity and decreasing acidity. This is of particular importance in the case of the studied low-productivity sandy acidic soils that have to be used in agriculture due to limited global land resources and rising food demand.

scholarly journals Highly diverse fungal communities in carbon-rich aquifers of two contrasting lakes in Northeast Germany

2019 ◽  
Author(s):  
Anita Perkins ◽  
Lars Ganzert ◽  
Keilor Rojas-Jiménez ◽  
Jeremy Fonvielle ◽  
Grant C. Hose ◽  
...  
Keyword(s):  
Organic Matter ◽  
Community Structure ◽  
Rrna Gene ◽  
28S Rrna ◽  
Ecological Role ◽  
Fungal Community Composition ◽  
Ne Germany ◽  
And Function ◽  
Carbon Processing ◽  
Complex Organic

AbstractFungi are an important component of microbial communities and are well known for their ability to degrade refractory, highly polymeric organic matter. In soils and aquatic systems, fungi play an important role in carbon processing, however, their diversity, community structure and function as well as ecological role, particularly in groundwater, are poorly studied. The aim of this study was to examine the fungal community composition, diversity and function of 16 groundwater boreholes located in the vicinity of two lakes in NE Germany that are characterized by contrasting trophic status. The analysis of 28S rRNA gene sequences amplified from the groundwater revealed high fungal diversity and clear differences in community structure between both aquifers. Most sequences were assigned to Ascomycota and Basidiomycota, but members of Chytridiomycota, Cryptomycota, Zygomycota, Blastocladiomycota, Glomeromycota and Neocallmastigomycota were also detected. In addition, 27 species of fungi were successfully isolated from the groundwater wells and tested for their ability to degrade complex organic polymers – the predominant carbon source in the wells. Most isolates showed positive activities for at least one of the tested polymer types, with three strains, belonging to the genera Gibberella, Isaria and Cadophora, being able to degrade all tested substrates. Our results highlight the high diversity of fungi in groundwater, and point to their important ecological role in breaking down highly polymeric organic matter in these isolated microbial habitats.

scholarly journals Potential effects of vinasse as a soil amendment to control runoff and soil loss

SOIL ◽  
2016 ◽  
Vol 2 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Z. Hazbavi ◽  
S. H. R. Sadeghi
Keyword(s):  
Organic Matter ◽  
Soil Loss ◽  
Soil Amendment ◽  
Organic Matter Content ◽  
Matter Content ◽  
Soil Restoration ◽  
Simulated Rainfall ◽  
Set Up ◽  
Loss Control ◽  
Runoff And Soil Loss

Abstract. Application of organic materials are well known as environmental practices in soil restoration, preserving soil organic matter and recovering degraded soils of arid and semiarid lands. Therefore, the present research focused on evaluating the effectiveness of vinasse, a byproduct mainly of the sugar-ethanol industry, on soil conservation under simulated rainfall. Vinasse can be recycled as a soil amendment due to its organic matter content. Accordingly, the laboratory experiments were conducted by using 0.25 m2 experimental plots at 20 % slope and rainfall intensity of 72 mm h−1 with 0.5 h duration. The effect of vinasse was investigated on runoff and soil loss control. Experiments were set up as a control (with no amendment) and three treated plots with doses of 0.5, 1, and 1.5 L m−2 of vinasse subjected to simulated rainfall. Laboratory results indicated that vinasse at different levels could not significantly (P >  0.05) decrease the runoff amount and soil loss rate in the study plots compared to untreated plots. The average amounts of minimum runoff volume and soil loss were about 3985 mL and 46 g for the study plot at a 1 L m−2 level of vinasse application.

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