scholarly journals A dynamic pattern of local auxin sources is required for root regeneration

2019 ◽  
Author(s):  
Rotem Matosevich ◽  
Itay Cohen ◽  
Naama Gil-Yarom ◽  
Abelardo Modrego ◽  
Carla Verna ◽  
...  
Keyword(s):  
Stem Cell Niche ◽  
Root Meristem ◽  
Root Regeneration ◽  
Competence Factor ◽  
Continuous Activity ◽  
Rapid Accumulation ◽  
Auxin Synthesis ◽  
Spatio Temporal ◽  
Cell Niche ◽  
Auxin Accumulation

AbstractFollowing removal of its stem cell niche, the root meristem can regenerate by recruitment of remnant cells from the stump. Regeneration is initiated by rapid accumulation of auxin near the injury site but the source of this auxin is unknown. Here, we show that auxin accumulation arises from the activity of multiple auxin biosynthetic sources that are newly specified near the cut site and that their continuous activity is required for the regeneration process. Auxin synthesis is highly localized and PIN-mediate transport is dispensable for auxin accumulation and tip regeneration. Roots lacking the activity of the regeneration competence factor ERF115, or that are dissected at a zone of low-regeneration potential, fail to activate local auxin sources. Remarkably, restoring auxin supply is sufficient to confer regeneration capacity to these recalcitrant tissues. We suggest that regeneration competence relies on the ability to specify new local auxin sources in a precise spatio-temporal pattern.

scholarly journals Week-long imaging of cell divisions in the Arabidopsis root meristem

2018 ◽  
Author(s):  
Ramin Rahni ◽  
Kenneth D. Birnbaum
Keyword(s):  
Stem Cell ◽  
Cell Division ◽  
Stem Cell Niche ◽  
Root Meristem ◽  
Imaging Method ◽  
Fluorescent Reporters ◽  
Cell Divisions ◽  
4D Imaging ◽  
Root Stem Cell ◽  
Cell Niche

AbstractCharacterizing the behaviors of dynamic systems requires capturing them with high temporal and spatial resolution. Owing to its transparency and genetic tractability, theArabidopsis thalianaroot lends itself well to live imaging when combined with cell and tissue-specific fluorescent reporters. We developed a novel 4D imaging method that utilizes simple confocal microscopy and readily available components to track cell divisions in the root stem cell niche and surrounding region for up to one week. This new setup allows us to finely analyze meristematic cell division rates that lead to patterning. Using this method, we performed a direct measurement of cell division intervals within and around the root stem cell niche. The results reveal a short, steep gradient of cell division in proximal stem cells, with progressively more rapid cell division rates from QC, to cells in direct contact with the QC (initials), to their immediate daughters, after which division rates appear to become more homogeneous. These results provide a baseline to study how perturbations in signaling could affect cell division patterns in the root meristem.

Tissue Stem Cells and Stem Cell Niche of the Human Vocal Fold

2020 ◽  
Vol 71 (2) ◽  
pp. 211-213
Author(s):  
K. Sato ◽  
S. Chitose ◽  
K. Sato ◽  
F. Sato ◽  
T. Kurita ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Niche ◽  
Vocal Fold ◽  
Cell Niche
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