scholarly journals Mapping Cell Membrane Organization and Dynamics Using Soft Nano-Imprint Lithography

2019 ◽  
Author(s):  
T. Sansen ◽  
D. Sanchez-Fuentes ◽  
R. Rathar ◽  
A. Colom-Diego ◽  
F. El Alaoui ◽  
...  
Keyword(s):  
Cell Membrane ◽  
High Performance ◽  
Protein Complexes ◽  
Membrane Mechanics ◽  
Nanostructured Surfaces ◽  
Cellular Processes ◽  
State Organization ◽  
Cell Membrane Mechanics ◽  
Membrane Shape

AbstractMembrane shape is a key feature of many cellular processes, including cell differentiation, division, migration, and trafficking. The development of nanostructured surfaces allowing for the in situ manipulation of membranes in living cells is crucial to understand these processes, but this requires complicated and limited-access technologies. Here, we investigate the self-organization of cellular membranes by using a customizable and bench top method allowing to engineer 1D SiO2 nanopillar arrays of defined sizes and shapes on high-performance glass compatible with advanced microscopies. As a result of this original combination, we provide a mapping of the morphology-induced modulation of the cell membrane mechanics, dynamics and steady-state organization of key protein complexes implicated in cellular trafficking and signal transduction.

scholarly journals A novel landscape of nuclear human CDK2 substrates revealed by in situ phosphorylation

2020 ◽  
Vol 6 (16) ◽  
pp. eaaz9899
Author(s):  
Yong Chi ◽  
John H. Carter ◽  
Jherek Swanger ◽  
Alexander V. Mazin ◽  
Robert L. Moritz ◽  
...  
Keyword(s):  
Cell Division ◽  
Protein Complexes ◽  
Nuclear Architecture ◽  
Cyclin Dependent Kinase ◽  
Oncogenic Signaling ◽  
Cellular Processes ◽  
Validation Rate ◽  
Associated Proteins ◽  
Epigenetic Regulators

Cyclin-dependent kinase 2 (CDK2) controls cell division and is central to oncogenic signaling. We used an “in situ” approach to identify CDK2 substrates within nuclei isolated from cells expressing CDK2 engineered to use adenosine 5′-triphosphate analogs. We identified 117 candidate substrates, ~40% of which are known CDK substrates. Previously unknown candidates were validated to be CDK2 substrates, including LSD1, DOT1L, and Rad54. The identification of many chromatin-associated proteins may have been facilitated by labeling conditions that preserved nuclear architecture and physiologic CDK2 regulation by endogenous cyclins. Candidate substrates include proteins that regulate histone modifications, chromatin, transcription, and RNA/DNA metabolism. Many of these proteins also coexist in multi-protein complexes, including epigenetic regulators, that may provide new links between cell division and other cellular processes mediated by CDK2. In situ phosphorylation thus revealed candidate substrates with a high validation rate and should be readily applicable to other nuclear kinases.

scholarly journals Dynamic mechanochemical feedback between curved membranes and BAR protein self-organization

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Anabel-Lise Le Roux ◽  
Caterina Tozzi ◽  
Nikhil Walani ◽  
Xarxa Quiroga ◽  
Dobryna Zalvidea ◽  
...  
Keyword(s):  
Molecular Organization ◽  
Self Organization ◽  
Membrane Mechanics ◽  
Curvature Sensing ◽  
Cellular Processes ◽  
Mechanical Responses ◽  
Curved Membranes ◽  
Comprehensive Framework ◽  
Membrane Shape ◽  
Template Size

AbstractIn many physiological situations, BAR proteins reshape membranes with pre-existing curvature (templates), contributing to essential cellular processes. However, the mechanism and the biological implications of this reshaping process remain unclear. Here we show, both experimentally and through modelling, that BAR proteins reshape low curvature membrane templates through a mechanochemical phase transition. This phenomenon depends on initial template shape and involves the co-existence and progressive transition between distinct local states in terms of molecular organization (protein arrangement and density) and membrane shape (template size and spherical versus cylindrical curvature). Further, we demonstrate in cells that this phenomenon enables a mechanotransduction mode, in which cellular stretch leads to the mechanical formation of membrane templates, which are then reshaped into tubules by BAR proteins. Our results demonstrate the interplay between membrane mechanics and BAR protein molecular organization, integrating curvature sensing and generation in a comprehensive framework with implications for cell mechanical responses.

scholarly journals Probing Cell Membrane Mechanics by Magnetic Particle Actuation and 3D Rotational Particle Tracking

2014 ◽  
Vol 106 (2) ◽  
pp. 43a
Author(s):  
Matthias Irmscher ◽  
Arthur M. de Jong ◽  
Holger Kress ◽  
Menno W.J Prins
Keyword(s):  
Cell Membrane ◽  
Particle Tracking ◽  
Magnetic Particle ◽  
Membrane Mechanics ◽  
Cell Membrane Mechanics

Studies of cochlear outer hair cell membrane mechanics using optical tweezers

2003 ◽  
Author(s):  
David R. Murdock ◽  
Sergey A. Ermilov ◽  
William E. Brownell ◽  
Bahman Anvari
Keyword(s):  
Cell Membrane ◽  
Hair Cell ◽  
Optical Tweezers ◽  
Outer Hair Cell ◽  
Membrane Mechanics ◽  
Cell Membrane Mechanics

Cell membrane mechanics and mechanosensory transduction

2020 ◽  
pp. 83-141
Author(s):  
Boris Martinac ◽  
Yury A. Nikolaev ◽  
Giulia Silvani ◽  
Navid Bavi ◽  
Valentin Romanov ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Mechanics ◽  
Mechanosensory Transduction ◽  
Cell Membrane Mechanics
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