scholarly journals 2D-HELS-AA MS Seq: Direct sequencing of tRNA reveals its different isoforms and multiple dynamic base modifications

2019 ◽  
Author(s):  
Ning Zhang ◽  
Shundi Shi ◽  
Xuanting Wang ◽  
Wenhao Ni ◽  
Xiaohong Yuan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Direct Method ◽  
Direct Sequencing ◽  
Full Length ◽  
Dynamic Changes ◽  
Variable Loop ◽  
Base Modifications ◽  
Truncated Isoforms ◽  
A Direct Method

AbstractWe report a direct method for sequencing tRNAPhe without cDNA by combining 2-dimensional hydrophobic RNA end-labeling with an anchor-based algorithm in mass spectrometry-based sequencing (2D-HELS-AA MS Seq). The entire tRNAPhe was sequenced and the identity, location and abundance of all 11 base modifications were determined. Changes in ratios of wybutosine and its depurinated form under different conditions were quantified, pointing to the ability of our technology to determine dynamic changes of nucleotide modifications. Two truncated isoforms at 3’CCA tail of the tRNAPhe (75 nt CC, 80% and 74 nt C, 3%) were identified in addition to the 76 nt tRNAPhe with a full-length 3’CCA tail (17%). We also discovered a new isoform with A-G transitions at both the 44 and 45 positions in the tRNAPhe variable loop.One Sentence SummaryDirect 2D-HELS-AA MS Seq of tRNA reveals different isoforms and base modifications

scholarly journals Direct determination of uranium and thorium in minerals by time-of-flight mass spectrometry with pulsed glow discharge

RSC Advances ◽  
2015 ◽  
Vol 5 (99) ◽  
pp. 80901-80910 ◽  
Author(s):  
Aleksandr Ganeev ◽  
Oksana Bogdanova ◽  
Ilia Ivanov ◽  
Boris Burakov ◽  
Natalia Agafonova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Glow Discharge ◽  
Direct Method ◽  
Time Of Flight ◽  
Direct Determination ◽  
Pulsed Glow Discharge ◽  
Flight Mass Spectrometry ◽  
Thorium Determination ◽  
A Direct Method

A direct method of uranium and thorium determination in non-conducting geological samples using time-of-flight mass spectrometry with pulsed glow discharge was proposed.

Laser ionization/time-of-flight mass spectrometry for the direct analysis of emulsions

2014 ◽  
Vol 6 (15) ◽  
pp. 5615-5619 ◽  
Author(s):  
Hidaka Ishigami ◽  
Yukihiro Tsuda ◽  
Tomohiro Uchimura
Keyword(s):  
Mass Spectrometry ◽  
Direct Method ◽  
Time Of Flight ◽  
Direct Analysis ◽  
Laser Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
A Direct Method

A direct method for the monitoring of emulsions was developed using laser ionization/time-of-flight mass spectrometry.

scholarly journals Plastic waste to fuels by hydrocracking at mild conditions

2021 ◽  
Vol 7 (17) ◽  
pp. eabf8283
Author(s):  
Sibao Liu ◽  
Pavel A. Kots ◽  
Brandon C. Vance ◽  
Andrew Danielson ◽  
Dionisios G. Vlachos
Keyword(s):  
Direct Method ◽  
Acid Sites ◽  
Liquid Fuels ◽  
High Yield ◽  
Tandem Catalysis ◽  
Hy Zeolite ◽  
Environmental Threat ◽  
Single Use ◽  
Initial Activation ◽  
A Direct Method

Single-use plastics impose an enormous environmental threat, but their recycling, especially of polyolefins, has been proven challenging. We report a direct method to selectively convert polyolefins to branched, liquid fuels including diesel, jet, and gasoline-range hydrocarbons, with high yield up to 85% over Pt/WO3/ZrO2 and HY zeolite in hydrogen at temperatures as low as 225°C. The process proceeds via tandem catalysis with initial activation of the polymer primarily over Pt, with subsequent cracking over the acid sites of WO3/ZrO2 and HY zeolite, isomerization over WO3/ZrO2 sites, and hydrogenation of olefin intermediates over Pt. The process can be tuned to convert different common plastic wastes, including low- and high-density polyethylene, polypropylene, polystyrene, everyday polyethylene bottles and bags, and composite plastics to desirable fuels and light lubricants.

Direct sequencing of a disulfide-linked peptide with electrospray ionization tandem mass spectrometry

The Analyst ◽  
2015 ◽  
Vol 140 (8) ◽  
pp. 2623-2627 ◽  
Author(s):  
Gongyu Li ◽  
Jiying Pei ◽  
Yue Yin ◽  
Guangming Huang
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Corona Discharge ◽  
Electrospray Ionization ◽  
Disulfide Bonds ◽  
Direct Sequencing ◽  
Peptide Sequencing ◽  
Tandem Mass ◽  
Esi Ms ◽  
Ionization Tandem Mass Spectrometry

Enhanced corona discharge was employed for in-spray dissociation of disulfide bonds, facilitating disulfide-containing peptide sequencing with ESI-MS/MS.

Sign in / Sign up

Export Citation Format

Share Document