scholarly journals Structural and functional analysis of genes with potential involvement in resistance to coffee leaf rust: a functional marker based approach

2019 ◽  
Author(s):  
Geleta Dugassa Barka ◽  
Eveline Teixeira Caixeta ◽  
Sávio Siqueira Ferreira ◽  
Laércio Zambolim
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Function Analysis ◽  
Bac Library ◽  
Leaf Rust Resistance ◽  
Molecular Data ◽  
Coffee Leaf Rust ◽  
Rust Resistance Genes

AbstractPhysiology-based differentiation of SH genes and Hemileia vastatrix races is the principal method employed for the characterization of coffee leaf rust resistance. Based on the gene-for-gene theory, nine major rust resistance genes (SH1-9) have been proposed. However, these genes have not been characterized at the molecular level. Consequently, the lack of molecular data regarding rust resistance genes or candidates is a major bottleneck in coffee breeding. To address this issue, we screened a BAC library with resistance gene analogs (RGAs), identified RGAs, characterized and explored for any SH related candidate genes. Herein, we report the identification and characterization of a gene (gene 11), which shares conserved sequences with other SH genes and displays a characteristic polymorphic allele conferring different resistance phenotypes. Furthermore, comparative analysis of the two RGAs belonging to CC-NBS-LRR revealed more intense diversifying selection in tomato and grape genomes than in coffee. For the first time, the present study has unveiled novel insights into the molecular nature of the SH genes, thereby opening new avenues for coffee rust resistance molecular breeding. The characterized candidate RGA is of particular importance for further biological function analysis in coffee.

scholarly journals Breeding of common wheat for leaf rust resistance using DNA markers

2020 ◽  
Author(s):  
E.R. Davoyan ◽  
◽  
L.A. Bespalova ◽  
R.O. Davoyan ◽  
E.V. Agaeva ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Common Wheat ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Selected Lines ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

The article presents the results of the characterization of 277 lines of common wheat developed in the National Center of Grain named after P.P. Lukyanenko by the presence of molecular markers linked to leaf rust resistance genes Lr9, Lr19, Lr24, Lr37, Lr26. Lines with Lr9 and Lr19 were not identified. We detected 52 lines carrying Lr24; 80 lines with Lr26; 141 lines with Lr37. Lines carrying a combination of leaf rust resistance genes were selected using molecular markers. The presence of a combination of Lr37 + Lr26 was established in 31 lines. The combination of Lr24 + Lr26 was detected in 12 lines. Line 125-15 Ms 2 carries a combination of Lr37 + Lr24. A pyramid of three genes was found in the line 144-15 Ms 2. Currently, the selected lines are widely involved in the breeding process.

scholarly journals Development of barley introgression lines carrying the leaf rust resistance genes Rph1 to Rph15

Crop Science ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 282-302 ◽  
Author(s):  
Matthew J. Martin ◽  
Oswaldo Chicaiza ◽  
Juan C. Caffarel ◽  
Ahmad H. Sallam ◽  
Arnis Druka ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Introgression Lines ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

scholarly journals Identification of Leaf Rust Resistance Genes in Selected Wheat Cultivars and Development of Multiplex PCR

2019 ◽  
Vol 14 (1) ◽  
pp. 327-334 ◽  
Author(s):  
Agnieszka Tomkowiak ◽  
Roksana Skowrońska ◽  
Alicja Buda ◽  
Danuta Kurasiak-Popowska ◽  
Jerzy Nawracała ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Field Resistance ◽  
Wheat Cultivars ◽  
Pcr Conditions ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

AbstractTen leading wheat cultivars originating from the Plant Breeding and Acclimatization Institute (IHAR) - National Research Institute (Poland) and the Department of Gene Bank (Czech Republic) were used to establish a field experiment in 2017 and 2018 at the Dłoń Experimental Farm. The analyzed wheat genotypes were characterized by diversified field resistance to leaf rust. Jubilatka, Thatcher and Sparta were the most resistant cultivars in field conditions in both 2017 and 2018. The aim of the work was to identify the Lr11, L13, Lr16 and Lr26 genes encoding resistance to leaf rust using molecular SSR markers (wmc24, wmc261, Xgwm630, Xwmc764 and P6M12) and to develop multiplex PCR conditions to accelerate identification of these genes. Markers of three leaf rust resistance genes have been identified simultaneously in these cultivars. Jubilatka, Thatcher and Sparta cultivars may serve as a good source of the analyzed leaf rust resistance genes. In addition, multiplex PCR conditions have been developed for the simultaneous identification of the Lr11 and Lr16 and Lr11 and Lr26 gene pairs.

Validating molecular markers for barley leaf rust resistance genes Rph5 and Rph7

2007 ◽  
Vol 126 (5) ◽  
pp. 458-463 ◽  
Author(s):  
J. A. Mammadov ◽  
W. S. Brooks ◽  
C. A. Griffey ◽  
M. A. Saghai Maroof
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Barley Leaf Rust ◽  
Barley Leaf ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

scholarly journals Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars

2013 ◽  
Vol 12 (9) ◽  
pp. 1580-1588 ◽  
Author(s):  
Li-hong SHI ◽  
Na ZHANG ◽  
Ya-ya HU ◽  
Xue-jun WEI ◽  
Wen-xiang YANG ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Spring Wheat ◽  
Resistance Genes ◽  
Chinese Spring ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Chinese Spring Wheat ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes
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