scholarly journals AFM based elasticity of intestinal epithelium correlate with barrier function under drug action

2019 ◽  
Author(s):  
H. Tejeda-Mora ◽  
L. Stevens ◽  
M. Gröllers ◽  
A. Katan ◽  
E. van de Steeg ◽  
...  
Keyword(s):  
Elastic Properties ◽  
Mechanism Of Action ◽  
Intestinal Epithelium ◽  
Drug Action ◽  
Point Of View ◽  
Clinical Use ◽  
Membrane Elasticity ◽  
Efficient Approach ◽  
Epithelial Membranes ◽  
Cellular Behaviour

ABSTRACTOver the past few years, atomic force microscopy (AFM) has developed as a mature research tool for measuring the nanomechanical properties of tissue, cells and biological structures. The force spectroscopy mode of AFM allows the local elasticity of biological samples to be measured. The mechanical properties of cells are highly affected by homeostatic changes observed during disease. In the case of the intestine, the aetiology for some conditions is still unclear. To improve the clinical translation of pre-clinical models, a new and different approach could be to study cellular behaviour in health and disease from a mechanical point of view. Specifically, knowledge of changes in epithelial membranes in response to drugs is useful for interpreting both drug action and disease development. Here, we used human intestinal Caco-2 cells as a first step to record epithelial membrane elasticity measurements at the nanoscale using AFM. Three different drugs were selected to influence intestinal epithelium integrity by specifically targeting different functional aspects of the membrane, such as permeability and support. Results indicate a relationship between measured cell elasticity and cell viability markers, such as cellular toxicity and membrane barrier functions. Our work represents a proof-of-concept that cells suffer a particular change in elastic properties depending upon the mechanism of action of an applied drug. The following may provide an efficient approach for diagnosing intestinal pathologies and testing drugs for clinical use.STATEMENT OF SIGNIFICANCEWe present evidence that epithelial membrane suffers a particular change in elastic properties depending upon the mechanism of action of an applied drug. These changes can be monitored over time using AFM technology and may provide an alternative and efficient approach for diagnosing intestinal pathologies and testing drugs for clinical use.

Application of Cis-Diamminedichloro-Platinum in Combination Antineoplastic Therapy of Testicular Cancer

1978 ◽  
Vol 12 (4) ◽  
pp. 226-229 ◽  
Author(s):  
Janice M. Williams ◽  
Nicholas G. Popovich
Keyword(s):  
Adverse Effects ◽  
Testicular Cancer ◽  
Antineoplastic Therapy ◽  
Mechanism Of Action ◽  
Combination Chemotherapy ◽  
Clinical Use ◽  
Adult Males ◽  
Testicular Carcinoma ◽  
Dosage Administration

Testicular carcinoma is a leading cause of cancer-related deaths in adult males between the ages of 20 and 35. Cis-Diamminedichloroplatinum (II) (i.e., CDDP) is currently being used investigatively in combination chemotherapy for the treatment of this disease. This article reviews the literature to date on CDDP and its application in testicular cancer. Individual CDDP parameters (e.g., chemistry, mechanism of action, effectiveness and clinical use, adverse effects, dosage, administration and distribution) are discussed.

scholarly journals A Review on the plants named after their karma (action) in Raja nighantu

2019 ◽  
Vol 6 (05) ◽  
pp. 4426-4432
Author(s):  
Prof. Karnam Chandrashekhar
Keyword(s):  
Mechanism Of Action ◽  
Pharmacological Action ◽  
Point Of View ◽  
15Th Century ◽  
Made In

What is there in the name? May be it is true philosophically, but, from Ayurvedic perspective especially from Nama rupa vijnana`s point of view, a lot is hidden in the name. Plants are not named just because they need the name, on the contrary, they help in understanding various aspects of the plant. The rationale of naming a plant is well documented in Raja nighantu. Though, there is a confusion about its time, yet, most of Ayurveda scholars believe it to be a work of 15th century. The pharmacological action of a plant is taken as a basis under the heading prabhava (effect) in naming a plant. This way of naming a plant helps a lot to have a look at its mechanism of action. For the reason, it is a great clue towards the pharmacodynamics of that plant and basis of many other plants working on the same line. Keeping this fact in mind, an earnest attempt is made in this review to explore the plants that are attributed with the names on the basis of their action in Raja nighantu. This review reveals 170 plants named after 36 activities with 153 synonyms.

scholarly journals Mechanism of Action of Icariin in Bone Marrow Mesenchymal Stem Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Aofei Yang ◽  
Chaochao Yu ◽  
Qilin Lu ◽  
Hao Li ◽  
Zhanghua Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Mechanism Of Action ◽  
Underlying Mechanism ◽  
Bone Defects ◽  
Clinical Use ◽  
Regenerative Therapy ◽  
Herba Epimedii ◽  
Marrow Mesenchymal Stem Cells

Osteoporosis, femoral head necrosis, and congenital bone defects are orthopedic disorders characterized by reduced bone generation and insufficient bone mass. Bone regenerative therapy primarily relies on the bone marrow mesenchymal stem cells (BMSCs) and their ability to differentiate osteogenically. Icariin (ICA) is the active ingredient of Herba epimedii, a common herb used in traditional Chinese medicine (TCM) formulations, and can effectively enhance BMSC proliferation and osteogenesis. However, the underlying mechanism of ICA action in BMSCs is not completely clear. In this review, we provide an overview of the studies on the role and mechanism of action of ICA in BMSCs, to provide greater insights into its potential clinical use in bone regeneration.

Antifungals

2016 ◽  
Author(s):  
M. Estée Török ◽  
Fiona J. Cooke ◽  
Ed Moran
Keyword(s):  
Mechanism Of Action ◽  
Mode Of Action ◽  
Antifungal Agents ◽  
Clinical Use ◽  
Mechanisms Of Resistance ◽  
Action Mechanisms

This chapter provides a systematic summary of antifungal agents, grouped by class and mechanism of action. Each summary provides information on the mode of action, mechanisms of resistance, pharmacology, and clinical use.

scholarly journals Recent Progress in Bioconjugation Strategies for Liposome-Mediated Drug Delivery

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5672
Author(s):  
Bethany Almeida ◽  
Okhil K. Nag ◽  
Katherine E. Rogers ◽  
James B. Delehanty
Keyword(s):  
Drug Delivery ◽  
Recent Progress ◽  
Recent Literature ◽  
Drug Carrier ◽  
Drug Loading ◽  
Drug Action ◽  
Clinical Use ◽  
Liposomal Drug ◽  
Specific Targeting

In nanoparticle (NP)-mediated drug delivery, liposomes are the most widely used drug carrier, and the only NP system currently approved by the FDA for clinical use, owing to their advantageous physicochemical properties and excellent biocompatibility. Recent advances in liposome technology have been focused on bioconjugation strategies to improve drug loading, targeting, and overall efficacy. In this review, we highlight recent literature reports (covering the last five years) focused on bioconjugation strategies for the enhancement of liposome-mediated drug delivery. These advances encompass the improvement of drug loading/incorporation and the specific targeting of liposomes to the site of interest/drug action. We conclude with a section highlighting the role of bioconjugation strategies in liposome systems currently being evaluated for clinical use and a forward-looking discussion of the field of liposomal drug delivery.

Clinical pharmacology of pyrroxane (proroxane)

2020 ◽  
Vol 18 (4) ◽  
pp. 335-350
Author(s):  
Petr D. Shabanov
Keyword(s):  
Clinical Pharmacology ◽  
Clinical Practice ◽  
Ergot Alkaloids ◽  
Drug Action ◽  
Drug Consumption ◽  
Clinical Use ◽  
Review Of Literature ◽  
Ministry Of Health ◽  
Adrenergic Blocker ◽  
Adrenergic Blockers

Review of literature and own studies on the pharmacology and clinical pharmacology of the domestic -adrenergic blocker pyrroxan (INN: proroxane). The drug was developed in the 1970s at the Institute of Toxicology of the USSR Ministry of Health (Leningrad). Expectations from -blockers created during this period (phenoxybenzamine, phentolamine, tropafen, fepracet, dihydrogenated ergot alkaloids) were high, especially in terms of the treatment of hypertensive and ischemic conditions, however, only few drugs from this group were used in clinical practice for these purposes (prazosin, doxazosin and some others). For pyrroxan, this direction was not the main one, and it was positioned as a means of treating diencephalic disorders like vegetative crises, where the drug immediately gained popularity, and this direction of use was clearly fixed to it. In the same period, the anti-alcohol and anti-drug action of -adrenergic blockers was found, since both in the experiment and in the clinic they demonstrated the suppression of alcohol (drug) motivation and a decrease in alcohol and drug consumption. Thelatter served as an impetus to the use of pyrroxane mainly in psychiatry, narcology and, to a lesser extent, in neurology, and recently the drug has been positioned almost exclusively for use in these areas of medicine. Thearticle analyzes the current clinical use of pyrroxane.

scholarly journals Social representations and information security of children and adolescents: the point of view of teachers (Part 1)

2017 ◽  
Vol 7 (1) ◽  
pp. 1-12 ◽  
Author(s):  
I.B. Bovina ◽  
N.V. Dvoryanchikov ◽  
S.Yu. Gayamova ◽  
A.V. Milekhin ◽  
S.V. Budykin
Keyword(s):  
Information Security ◽  
Children And Adolescents ◽  
Mobile Phones ◽  
Mechanism Of Action ◽  
New Technologies ◽  
Social Representations ◽  
Focus Of Attention ◽  
Point Of View ◽  
The Internet ◽  
Theory Of Social Representations

The focus of attention of the authors is the consideration of information security of children and adolescents as the object of social representations. Discusses the need for the theory of social representations to develop prophylactic and preventive programs in the field of information security of children and adolescents, in particular, says that the effectiveness of prevention and preventive programs is determined by how this information is understood by the target group, through what filters it passes. Briefly outlines the main provisions of the theory of social representations, discusses the functions of social representations, the mechanism of action ideas. Analyzed in a few studies, the focus of which was the presentation on new technologies, particularly mobile phones and the Internet. Presents the results of a series of studies devoted to the study of information security of children and adolescents, including the results of the first part of a research project dedicated to the analysis of how parents and teachers understand information security and what strategies to protect children and adolescents from dangerous information they are ready to use.

scholarly journals Baricitinib in the Treatment of Patients with COVID-19: A Review of International Data and Analysis of Clinical Results in the Russian Population

2021 ◽  
Vol 66 (1-2) ◽  
pp. 47-56
Author(s):  
А. I. Zagrebneva ◽  
Е. А. Baryakh ◽  
Е. I. Zhelnova ◽  
N. G. Poteshkina ◽  
I. Р. Beloglazova ◽  
...  
Keyword(s):  
Clinical Results ◽  
Point Of View ◽  
Janus Kinase ◽  
Early Warning Score ◽  
Reversible Inhibitor ◽  
Clinical Use ◽  
Blood Oxygen Saturation ◽  
Mean Values ◽  
Average Value ◽  
Pathogenetic Therapy

The effects of baricitinib, a selective reversible inhibitor of Janus kinase 1 and 2, in the treatment of COVID-19 are associated with different aspects of pathogenesis — inhibition of viral endocytosis, reduction of excessive inflammatory response, and mitigation of vascular and pulmonary damage, which is a strong rationale for using baricitinib to treat patients with COVID-19. In the period from April to May 2020, City Clinical Hospital No. 52 obtained clinical experience of baricitinib clinical use in the therapy of 113 patients with COVID-19: 58 (51%) women and 55 (49%) men, whose average age was 57±12.6 years old. Analysis of the results of using baricitinib showed that therapy with baricitinib against the background of standard pathogenetic therapy was found to be effective in 95 (84%) patients and ineffective in 18 (16%). Significant positive changes were shown in comparison with the baseline level of the following indicators: body temperature (from 37.2±0.8˚C to 36, ±0.68˚C, P=0.000), blood oxygen saturation (from 95.5±3.0% to 96.5±2.2%, P=0.011), C-reactive protein (from 46.1±48.0 mg/L to 33.5±43.7 mg/L, P=0.010 ), National Early Warning Score (NEWS) (from 1.7±1.3 to 1.1±1.2, p=0.001). From the safety point of view, patients showed a slight decrease in the average value of the number of neutrophils — from (3.1±1.4)×109 to (3.0±2.0)×109 and lymphocytes — from (1.8±0,9)×109 to (1.7±0.9)×109, as well as minimal multidirectional changes in the mean values of transaminase activity — alanine aminotransferase changed from 33.9±23.6 U/L to 34.9±47.5 U/L, aspartate aminotransferase — from 40.6±49.0 U/L to 38.5±25.5 U/L. In general, the results obtained within the experience of the clinical use of baricitinib in 113 Russian patients with COVID-19 are consistent with the available data from foreign clinical studies and confirm the efficacy and safety of baricitinib.  

STUDIES ON THE EFFECTS OF PHAGOCYTIC STIMULATION ON MICROBIAL DISEAS: VI. SOME FACTORS DECREASING PHAGOCYTIC ACTIVITY OF MONOCYTES AGAINST TUBERCLE BACILLI, STRAIN BCG

1956 ◽  
Vol 34 (3) ◽  
pp. 580-586
Author(s):  
László Kátó ◽  
Béla Gözsy
Keyword(s):  
Mechanism Of Action ◽  
Phagocytic Activity ◽  
Point Of View ◽  
Experimental Tuberculosis

An antihistamine if present in a concentration of 0.1 to 100 μgm. per ml. decreased considerably the in vitro phagocytic activity of normal monocytes. The stimulated phagocytic activity of monocytes by histamine or 1,4-dimethyl-7-isopropyl-bicyclo-decapentane was antagonized by the antihistamines. Monocytes withdrawn from antihistamine treated animals showed a markedly lesser phagocytic activity than monocytes from untreated animals. The results are discussed from the point of view of the mechanism of action of 1,4-dimethyl-7-isopropyl-bicyclo-decapentane in experimental tuberculosis.

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