scholarly journals Exploration of social spreading reveals behavior is prevalent among Pedobacter and P. fluorescens isolates, and shows variations in induction of phenotype

2019 ◽  
Author(s):  
Lucy M. McCully ◽  
Jasmine Graslie ◽  
Adam S. Bitzer ◽  
Auður M. Sigurbjörnsdóttir ◽  
Oddur Vilhelmsson ◽  
...  
Keyword(s):  
Social Context ◽  
Microbial Communities ◽  
Soil Bacteria ◽  
Close Contact ◽  
Microbial Interactions ◽  
Emergent Properties ◽  
High Nutrient ◽  
Agar Surface ◽  
Nutritional Environment ◽  
Emergent Community

AbstractWithin soil, bacteria are naturally found in multi-species communities, where interactions can lead to emergent community properties. It is critical that we study bacteria in a social context to investigate community-level functions. We previously showed that when co-cultured, Pseudomonas fluorescens Pf0-1 and Pedobacter sp. V48 engage in interspecies social spreading on a hard agar surface, a behavior which required close contact and was dependent on the nutritional environment. In this study, we investigate whether the ability to participate in social spreading is widespread among P. fluorescens and Pedobacter isolates, and whether the requirements for interaction vary. We find that this phenotype is not restricted to the interaction between P. fluorescens Pf0-1 and Pedobacter sp. V48, but is a more prevalent behavior found in one clade in the P. fluorescens group and two clades in the Pedobacter genus. We also discovered that the interaction with certain Pedobacter isolates occurred without close contact, indicating induction of spreading by a putative diffusible signal. As is the case for ISS by Pf0-1+V48, motility of all interacting pairs is influenced by the environment, with no spreading behaviors observed under high nutrient conditions. While Pf0-1+V48 require low nutrient but high NaCl conditions, in the broader range of interacting pairs this requirement for low nutrient and high salt was variable. The prevalence of motility phenotypes observed in this study and found within the literature indicates that community-induced locomotion in general, and social spreading in particular, is likely important within the environment. It is crucial that we continue to study microbial interactions and their emergent properties to gain a fuller understanding of the functions of microbial communities.

scholarly journals Exploration of social spreading reveals behavior is prevalent among Pedobacter and P. fluorescens isolates, and shows variations in induction of phenotype

2021 ◽  
Author(s):  
Lucy M. McCully ◽  
Jasmine Graslie ◽  
Alana R. McGraw ◽  
Adam S. Bitzer ◽  
Auður M. Sigurbjörnsdóttir ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Close Contact ◽  
Soil Microbial Communities ◽  
Group Behavior ◽  
Microbial Interactions ◽  
Emergent Behavior ◽  
Individual Species ◽  
Emergent Properties ◽  
Community Function ◽  
The Individual

Within soil, bacteria are found in multi-species communities, where interactions can lead to emergent community properties. Studying bacteria in a social context is critical for investigation of community-level functions. We previously showed that co-cultured Pseudomonas fluorescens Pf0-1 and Pedobacter sp. V48 engage in interspecies social spreading (ISS) on a hard agar surface, a behavior which required close contact and depended on the nutritional environment. Here, we investigate whether social spreading is widespread among P. fluorescens and Pedobacter isolates, and whether the requirements for interaction vary. We find that this phenotype is not restricted to the interaction between P. fluorescens Pf0-1 and Pedobacter sp. V48, but is a prevalent behavior found in one clade in the P. fluorescens group and two clades in the Pedobacter genus. We show that the interaction with certain Pedobacter isolates occurred without close contact, indicating induction of spreading by a putative diffusible signal. As with ISS by Pf0-1+V48, motility of interacting pairs is influenced by the environment, with no spreading behaviors (or induction of motility) observed under high nutrient conditions. While Pf0-1+V48 require low nutrient but high NaCl conditions, in the broader range of interacting pairs the high salt influence was variable. The prevalence of motility phenotypes observed here and found within the literature indicates that community-induced locomotion in general, and social spreading in particular, is likely important within the environment. It is crucial that we continue to study microbial interactions and their emergent properties to gain a fuller understanding of the functions of microbial communities. Importance Interspecies social spreading (ISS) is an emergent behavior observed when P. fluorescens Pf0-1 and Pedobacter sp. V48 interact, during which both species move together across a surface. Importantly, this environment does not permit movement of either individual species. This group behavior suggests that communities of microbes can function in ways not predictable by knowledge of the individual members. Here we have asked whether ISS is widespread and thus potentially of importance in soil microbial communities. The significance of this research is the demonstration that surface spreading behaviors are not unique to the Pf0-1-V48 interaction, but rather is a more widespread phenomenon observed among members of distinct clades of both P. fluorescens and Pedobacter isolates. Further, we identify differences in mechanism of signaling and nutritional requirements for ISS. Emergent traits resulting from bacterial interactions are widespread and their characterization is necessary for a complete understanding of microbial community function.

scholarly journals Interspecies Social Spreading: Interaction between two sessile soil bacteria leads to emergence of surface motility

2018 ◽  
Author(s):  
Lucy M. McCully ◽  
Adam S. Bitzer ◽  
Sarah C. Seaton ◽  
Leah M. Smith ◽  
Mark W. Silby
Keyword(s):  
Microbial Communities ◽  
Pseudomonas Fluorescens ◽  
Soil Bacteria ◽  
Interspecific Interactions ◽  
Close Association ◽  
Soil Microbiome ◽  
Emergent Properties ◽  
Community Members ◽  
Holistic Understanding ◽  
Nutrient Conditions

AbstractBacteria often live in complex communities in which they interact with other organisms. Consideration of the social environment of bacteria can reveal emergent traits and behaviors that would be overlooked by studying bacteria in isolation. Here we characterize a social trait which emerges upon interaction between the distantly-related soil bacteriaPseudomonas fluorescensPf0-1 andPedobactersp. V48. On hard agar, which is not permissive for motility of the mono-culture of either species, co-culture reveals an emergent phenotype we term ‘interspecies social spreading,’ where the mixed colony spreads across the hard surface. We show that initiation of social spreading requires close association between the two species of bacteria. Both species remain associated throughout the spreading colony, with reproducible and non-homogenous patterns of distribution. The nutritional environment influences social spreading; no social behavior is observed under high nutrient conditions, but low nutrient conditions are insufficient to promote social spreading without high salt concentrations. This simple two-species consortium is a tractable model system that will facilitate mechanistic investigations of interspecies interactions and provide insight into emergent properties of interacting species. These studies will contribute to the broader knowledge of how bacterial interactions influence the functions of communities they inhabit.ImportanceThe wealth of studies on microbial communities has revealed the complexity and dynamics of the composition of communities in many ecological settings. Fewer studies probe the functional interactions of the community members. Function of the community as a whole may not be fully revealed by characterizing the individuals. In our two-species model community, we find an emergent trait resulting from the interaction of the soil bacteriaPseudomonas fluorescensPf0-1 andPedobactersp. V48. Observation of emergent traits suggests there may be many functions of a community that are not predicted based ona prioriknowledge of the community members. These types of studies will provide a more holistic understanding of microbial communities, allowing us to connect information about community composition with behaviors determined by interspecific interactions. These studies increase our ability to understand communities, such as the soil microbiome, plant-root microbiome, and human gut microbiome, with the final goal of being able to manipulate and rationally improve these communities.

scholarly journals Interspecies Social Spreading: Interaction between Two Sessile Soil Bacteria Leads to Emergence of Surface Motility

mSphere ◽  
2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Lucy M. McCully ◽  
Adam S. Bitzer ◽  
Sarah C. Seaton ◽  
Leah M. Smith ◽  
Mark W. Silby
Keyword(s):  
Microbial Communities ◽  
Pseudomonas Fluorescens ◽  
Soil Bacteria ◽  
Interspecific Interactions ◽  
Soil Microbiome ◽  
Emergent Properties ◽  
Community Members ◽  
Content Type ◽  
Holistic Understanding ◽  
Nutrient Conditions

ABSTRACTBacteria often live in complex communities in which they interact with other organisms. Consideration of the social environment of bacteria can reveal emergent traits and behaviors that would be overlooked by studying bacteria in isolation. Here we characterize a social trait which emerges upon interaction between the distantly related soil bacteriaPseudomonas fluorescensPf0-1 andPedobactersp. strain V48. On hard agar, which is not permissive for motility of the monoculture of either species, coculture reveals an emergent phenotype that we term “interspecies social spreading,” where the mixed colony spreads across the hard surface. We show that initiation of social spreading requires close association between the two species of bacteria. Both species remain associated throughout the spreading colony, with reproducible and nonhomogenous patterns of distribution. The nutritional environment influences social spreading: no social behavior is observed under high-nutrient conditions, but low-nutrient conditions are insufficient to promote social spreading without high salt concentrations. This simple two-species consortium is a tractable model system that will facilitate mechanistic investigations of interspecies interactions and provide insight into emergent properties of interacting species. These studies will contribute to the broader knowledge of how bacterial interactions influence the functions of communities they inhabit.IMPORTANCEThe wealth of studies on microbial communities has revealed the complexity and dynamics of the composition of communities in many ecological settings. Fewer studies probe the functional interactions of the community members. Function of the community as a whole may not be fully revealed by characterizing the individuals. In our two-species model community, we find an emergent trait resulting from the interaction of the soil bacteriaPseudomonas fluorescensPf0-1 andPedobactersp. V48. Observation of emergent traits suggests there may be many functions of a community that are not predicted based ona prioriknowledge of the community members. These types of studies will provide a more holistic understanding of microbial communities, allowing us to connect information about community composition with behaviors determined by interspecific interactions. These studies increase our ability to understand communities, such as the soil microbiome, plant-root microbiome, and human gut microbiome, with the final goal of being able to manipulate and rationally improve these communities.

A peek in the micro-sized world: a review of design principles, engineering tools, and applications of engineered microbial community

2020 ◽  
Vol 48 (2) ◽  
pp. 399-409
Author(s):  
Baizhen Gao ◽  
Rushant Sabnis ◽  
Tommaso Costantini ◽  
Robert Jinkerson ◽  
Qing Sun
Keyword(s):  
Microbial Community ◽  
Microbial Communities ◽  
Environmental Remediation ◽  
Real Life ◽  
Design Principles ◽  
Microbial Interactions ◽  
Potential Applications ◽  
New Gene ◽  
Global Biogeochemical Cycles ◽  
Synthetic Microbial Communities

Microbial communities drive diverse processes that impact nearly everything on this planet, from global biogeochemical cycles to human health. Harnessing the power of these microorganisms could provide solutions to many of the challenges that face society. However, naturally occurring microbial communities are not optimized for anthropogenic use. An emerging area of research is focusing on engineering synthetic microbial communities to carry out predefined functions. Microbial community engineers are applying design principles like top-down and bottom-up approaches to create synthetic microbial communities having a myriad of real-life applications in health care, disease prevention, and environmental remediation. Multiple genetic engineering tools and delivery approaches can be used to ‘knock-in' new gene functions into microbial communities. A systematic study of the microbial interactions, community assembling principles, and engineering tools are necessary for us to understand the microbial community and to better utilize them. Continued analysis and effort are required to further the current and potential applications of synthetic microbial communities.

scholarly journals Children’s Pictorial Expression of Plant Life and Its Connection with School-Based Greenness

2021 ◽  
Vol 13 (9) ◽  
pp. 4999
Author(s):  
Ilargi Zaballa ◽  
Maria Merino ◽  
José Domingo Villarroel
Keyword(s):  
Social Context ◽  
Conceptual Development ◽  
Positive Impact ◽  
Close Contact ◽  
Positive Influence ◽  
Surrounding Area ◽  
School Site ◽  
School Based ◽  
Plant Life ◽  
Green Areas

Previous research highlights the positive influence that experiences in nature have on children’s physical, emotional and conceptual development. There is also evidence that indicates that the availability of green areas on school grounds is associated with pupils’ better academic performance as well as with their comprehension of wildlife. This study examines the drawings that 152 children completed with the objective of expressing their understanding of the plant world. Approximately half of the drawings were depicted by children that attend a school with green areas within the school site as well as in the surrounding area. The remaining half of the sample includes the illustrations that children attending an educational centre with, virtually, no green areas within the school premises or in the immediate vicinity. Notwithstanding the fact that the two schools involved in the study belong to a similar social context and they are relatively close to each other, the results show relevant differences between the drawings by the two groups compared, in terms of the pictorial content and the utilisation of colour. The results are discussed in light of the growing number of studies that emphasise the positive impact that close contact with nature has on children’s everyday life at school.

scholarly journals The Microbiome Modeling Toolbox: from microbial interactions to personalized microbial communities

2018 ◽  
Vol 35 (13) ◽  
pp. 2332-2334 ◽  
Author(s):  
Federico Baldini ◽  
Almut Heinken ◽  
Laurent Heirendt ◽  
Stefania Magnusdottir ◽  
Ronan M T Fleming ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Microbial Interactions ◽  
Microbial Genome ◽  
Metagenomic Data ◽  
Metabolic Interactions ◽  
Constraint Based Modeling ◽  
Genome Scale

Abstract Motivation The application of constraint-based modeling to functionally analyze metagenomic data has been limited so far, partially due to the absence of suitable toolboxes. Results To address this gap, we created a comprehensive toolbox to model (i) microbe–microbe and host–microbe metabolic interactions, and (ii) microbial communities using microbial genome-scale metabolic reconstructions and metagenomic data. The Microbiome Modeling Toolbox extends the functionality of the constraint-based reconstruction and analysis toolbox. Availability and implementation The Microbiome Modeling Toolbox and the tutorials at https://git.io/microbiomeModelingToolbox.

scholarly journals Deciphering the evolution of microbial interactions: in silico studies of two-member microbial communities

2022 ◽  
Author(s):  
Gayathri Sambamoorthy ◽  
Karthik Raman
Keyword(s):  
Microbial Communities ◽  
In Silico ◽  
Microbial Interactions ◽  
Interaction Patterns ◽  
Wild Type ◽  
Community Function ◽  
Evolutionary Forces ◽  
In Silico Studies ◽  
Wide Range ◽  
In The Wild

Microbes thrive in communities, embedded in a complex web of interactions. These interactions, particularly metabolic interactions, play a crucial role in maintaining the community structure and function. As the organisms thrive and evolve, a variety of evolutionary processes alter the interactions among the organisms in the community, although the community function remains intact. In this work, we simulate the evolution of two-member microbial communities in silico to study how evolutionary forces can shape the interactions between organisms. We employ genomescale metabolic models of organisms from the human gut, which exhibit a range of interaction patterns, from mutualism to parasitism. We observe that the evolution of microbial interactions varies depending upon the starting interaction and also on the metabolic capabilities of the organisms in the community. We find that evolutionary constraints play a significant role in shaping the dependencies of organisms in the community. Evolution of microbial communities yields fitness benefits in only a small fraction of the communities, and is also dependent on the interaction type of the wild-type communities. The metabolites cross-fed in the wild-type communities appear in only less than 50% of the evolved communities. A wide range of new metabolites are cross-fed as the communities evolve. Further, the dynamics of microbial interactions are not specific to the interaction of the wild-type community but vary depending on the organisms present in the community. Our approach of evolving microbial communities in silico provides an exciting glimpse of the dynamics of microbial interactions and offers several avenues for future investigations.

p-Coumaric can alter the composition of cucumber rhizosphere microbial communities and induce negative plant-microbial interactions

2018 ◽  
Vol 54 (3) ◽  
pp. 363-372 ◽  
Author(s):  
Xingang Zhou ◽  
Jianhui Zhang ◽  
Dandan Pan ◽  
Xin Ge ◽  
Xue Jin ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Microbial Interactions
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