Hedgehog signaling regulates neurogenesis in the larval and adult zebrafish hypothalamus

Mapping Intimacies ◽

10.1101/740613 ◽

2019 ◽

Author(s):

Ira Male ◽

A. Tuba Ozacar ◽

Rita R. Fagan ◽

Matthew Loring ◽

Meng-Chieh Shen ◽

...

Keyword(s):

Cell Signaling ◽

Hedgehog Signaling ◽

Radial Glia ◽

Proper Function ◽

Adult Zebrafish ◽

Evolutionarily Conserved ◽

Slow Cycling ◽

Necessary And Sufficient ◽

First Time ◽

Cell Cell

AbstractWhile neurogenesis in the adult hypothalamus is now known to be essential for proper function, the cell-cell signaling events that regulate neurogenesis in this evolutionarily conserved brain region remain poorly understood. Here we show that Hedgehog (Hh)/Gli signaling positively regulates hypothalamic neurogenesis in both larval and adult zebrafish and is necessary and sufficient for normal hypothalamic proliferation rates. Hedgehog-responsive cells are relatively rapidly proliferating pluripotent neural precursors that give rise to dopaminergic, serotonergic, and GABAergic neurons. in situ and transgenic reporter analyses revealed substantial heterogeneity in cell-cell signaling within the hypothalamic niche, with slow cycling Nestin-expressing cells residing among distinct and overlapping populations of Sonic Hh (Shh)-expressing, Hh-responsive, Notch-responsive, and Wnt-responsive radial glia. This work shows for the first time that Hh/Gli-signaling is a key component of the complex cell-cell signaling environment that regulates hypothalamic neurogenesis throughout life.

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Biphasic roles of hedgehog signaling in the production and self-renewal of outer radial glia in the ferret cerebral cortex

10.1101/2021.04.12.439562 ◽

2021 ◽

Author(s):

Shirui Hou ◽

Wan-Ling Ho ◽

Lei Wang ◽

Bryan Kuo ◽

Jun Young Park ◽

...

Keyword(s):

Hedgehog Signaling ◽

Radial Glia ◽

Superficial Layer ◽

Cellular Mechanisms ◽

Self Renewal ◽

Layer Neuron ◽

Outer Radial Glia ◽

Hh Signaling ◽

Necessary And Sufficient

The neocortex, the center for higher brain function, emerged in mammals and expanded in the course of evolution. The expansion of outer radial glia (oRGs) and intermediate progenitor cells (IPCs) plays key roles in the expansion and consequential folding of the neocortex. Therefore, understanding the mechanisms of oRG and IPC expansion is important for understanding neocortical development and evolution. By using mice and human cerebral organoids, we previously revealed that hedgehog (HH) signaling expands oRGs and IPCs. Nevertheless, it remained to be determined whether HH signaling expanded oRGs and IPCs in vivo in gyrencephalic species, in which oRGs and IPCs are naturally expanded. Here, we show that HH signaling is necessary and sufficient to expand oRGs and IPCs in ferrets, a gyrencephalic species, through conserved cellular mechanisms. HH signaling increases oRG-producing division modes of ventricular radial glia (vRGs), oRG self-renewal, and IPC proliferation. Notably, HH signaling affects vRG division modes only in an early restricted phase before superficial-layer neuron production peaks. Beyond this restricted phase, HH signaling promotes oRG self-renewal. Thus, HH signaling expands oRGs and IPCs in two distinct but continuous phases during cortical development.

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Faculty Opinions recommendation of Programming self-organizing multicellular structures with synthetic cell-cell signaling.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.733320732.793550297 ◽

2018 ◽

Author(s):

Rino Rappuoli

Keyword(s):

Cell Signaling ◽

Synthetic Cell ◽

Cell Cell ◽

Self Organizing

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Interest rate option hedging portfolios without bank account

Studies in Economics and Finance ◽

10.1108/sef-02-2019-0058 ◽

2019 ◽

Vol 37 (1) ◽

pp. 134-142

Author(s):

Alberto Bueno-Guerrero

Keyword(s):

Design Methodology ◽

Contingent Claim ◽

Necessary And Sufficient Condition ◽

Barrier Option ◽

Bank Account ◽

Content Type ◽

Option Hedging ◽

Hedging Portfolio ◽

Necessary And Sufficient ◽

First Time

Purpose This paper aims to study the conditions for the hedging portfolio of any contingent claim on bonds to have no bank account part. Design/methodology/approach Hedging and Malliavin calculus techniques recently developed under a stochastic string framework are applied. Findings A necessary and sufficient condition for the hedging portfolio to have no bank account part is found. This condition is applied to a barrier option, and an example of a contingent claim whose hedging portfolio has a bank account part different from zero is provided. Originality/value To the best of the authors’ knowledge, this is the first time that this issue has been addressed in the literature.

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Integration of AI-2 Based Cell-Cell Signaling with Metabolic Cues in Escherichia coli

PLoS ONE ◽

10.1371/journal.pone.0157532 ◽

2016 ◽

Vol 11 (6) ◽

pp. e0157532 ◽

Cited By ~ 8

Author(s):

Arindam Mitra ◽

Christopher D. Herren ◽

Isha R. Patel ◽

Adam Coleman ◽

Suman Mukhopadhyay

Keyword(s):

Escherichia Coli ◽

Cell Signaling ◽

Cell Cell

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C-factor: A cell-cell signaling protein required for fruiting body morphogenesis of M. Xanthus

Cell ◽

10.1016/0092-8674(90)90211-v ◽

1990 ◽

Vol 61 (1) ◽

pp. 19-26 ◽

Cited By ~ 142

Author(s):

Seung K. Kim ◽

Dale Kaiser

Keyword(s):

Cell Signaling ◽

Fruiting Body ◽

Signaling Protein ◽

A Cell ◽

Cell Cell

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Cell-Cell Signaling within Crown Gall Tumors

Chemical Communication among Bacteria ◽

10.1128/9781555815578.ch18 ◽

2014 ◽

pp. 291-306 ◽

Cited By ~ 2

Author(s):

Stephen C. Winans

Keyword(s):

Cell Signaling ◽

Crown Gall ◽

Crown Gall Tumors ◽

Cell Cell

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Faculty Opinions recommendation of Cilium-independent regulation of Gli protein function by Sufu in Hedgehog signaling is evolutionarily conserved.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1164273.625981 ◽

2009 ◽

Author(s):

James Briscoe

Keyword(s):

Protein Function ◽

Hedgehog Signaling ◽

Evolutionarily Conserved

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Osteocalcin expressing cells from tendon sheaths in mice contribute to tendon repair by activating Hedgehog signaling

eLife ◽

10.7554/elife.30474 ◽

2017 ◽

Vol 6 ◽

Cited By ~ 12

Author(s):

Yi Wang ◽

Xu Zhang ◽

Huihui Huang ◽

Yin Xia ◽

YiFei Yao ◽

...

Keyword(s):

Progenitor Cells ◽

Hedgehog Signaling ◽

Progenitor Cell ◽

Tendon Repair ◽

Tendon Sheath ◽

Lineage Tracing ◽

Specific Marker ◽

Molecular Function ◽

Hh Signaling ◽

Necessary And Sufficient

Both extrinsic and intrinsic tissues contribute to tendon repair, but the origin and molecular functions of extrinsic tissues in tendon repair are not fully understood. Here we show that tendon sheath cells harbor stem/progenitor cell properties and contribute to tendon repair by activating Hedgehog signaling. We found that Osteocalcin (Bglap) can be used as an adult tendon-sheath-specific marker in mice. Lineage tracing experiments show that Bglap-expressing cells in adult sheath tissues possess clonogenic and multipotent properties comparable to those of stem/progenitor cells isolated from tendon fibers. Transplantation of sheath tissues improves tendon repair. Mechanistically, Hh signaling in sheath tissues is necessary and sufficient to promote the proliferation of Mkx-expressing cells in sheath tissues, and its action is mediated through TGFβ/Smad3 signaling. Furthermore, co-localization of GLI1+ and MKX+ cells is also found in human tendinopathy specimens. Our work reveals the molecular function of Hh signaling in extrinsic sheath tissues for tendon repair.

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