scholarly journals Cornichon Homolog-3 (CNIH3) Modulates Spatial Memory in Female Mice

2019 ◽  
Author(s):  
Hannah E. Frye ◽  
Sidney B. Williams ◽  
Christopher R. Trousdale ◽  
Elliot C. Nelson ◽  
Joseph D. Dougherty ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
Spatial Memory ◽  
Dorsal Hippocampus ◽  
Postsynaptic Membrane ◽  
Spatial Learning And Memory ◽  
Female Mice ◽  
Specific Effects ◽  
Hippocampal Synaptic Plasticity ◽  
Auxiliary Protein

ABSTRACTCornichon homolog-3 (CNIH3) is an AMPA receptor (AMPAR) auxiliary protein that traffics AMPARs to the postsynaptic membrane and potentiates AMPAR signaling. AMPARs are key components of hippocampal synaptic plasticity and memory formation, however the role of CNIH3 in memory has yet to be elucidated. To study the role of CNIH3 on mouse behavior, we bred and characterized a line of Cnih3-/- mice from C57BL/6 Cnih3tm1a(KOMP)Wtsi mice obtained from the Knockout Mouse Project (KOMP). In agreement with previous studies of CNIH3 in the brain, we observed concentrated expression of Cnih3 in the dorsal hippocampus, a region associated with spatial learning and memory. Therefore, we tested Cnih3+/+, Cnih3+/-, and Cnih3-/- mice in the Barnes maze paradigm to measure spatial memory. We observed no change in spatial memory in male Cnih3+/- and Cnih3-/- mice compared to male Cnih3+/+ controls, however, Cnih3-/- female mice made significantly more primary errors, had a higher primary latency, and took less efficient routes to the target in the maze compared to Cnih3+/+ female mice. Next, to investigate an enhancement of spatial memory by Cnih3 overexpression, specifically in the dorsal hippocampus, we developed an AAV5 viral construct to express wild-type Cnih3 in excitatory neurons. Female mice overexpressing Cnih3 made significantly fewer errors, had a lower primary latency to the target, and took more efficient routes to the maze target compared to YFP expressing control females. No change in spatial memory was observed in male Cnih3 overexpression mice. This study, the first to identify sex-specific effects of the AMPAR auxiliary protein CNIH3 on spatial memory, provides the groundwork for future studies investigating the role of CNIH3 on sexually dimorphic AMPAR-dependent behavior and hippocampal synaptic plasticity.

scholarly journals Pyk2 in dorsal hippocampus plays a selective role in spatial memory and synaptic plasticity

2021 ◽  
Author(s):  
Vincenzo Mastrolia ◽  
Omar al Massadi ◽  
Benoit de Pins ◽  
Jean-Antoine Girault
Keyword(s):  
Synaptic Plasticity ◽  
Spatial Memory ◽  
Dorsal Hippocampus ◽  
Training Session ◽  
Long Term Potentiation ◽  
Object Location ◽  
High Frequency Stimulation ◽  
Novel Object ◽  
Ca1 Region

Pyk2 is a Ca2+-activated non-receptor tyrosine kinase enriched in the forebrain, especially in pyramidal neurons of the hippocampus. Previous reports suggested its role in hippocampal synaptic plasticity and spatial memory but with contradictory findings possibly due to experimental conditions. Here we address this issue and show that novel object location, a simple test of spatial memory induced by a single training session, is altered in Pyk2 KO mice and that re-expression of Pyk2 in the dorsal hippocampus corrects this deficit. Bilateral targeted deletion of Pyk2 in dorsal hippocampus CA1 region also alters novel object location. Long term potentiation (LTP) in CA1 is impaired in Pyk2 KO mice using a high frequency stimulation induction protocol nut not with a theta burst protocol, explaining differences between previous reports. The same selective LTP alteration is observed in mice with Pyk2 deletion in dorsal hippocampus CA1 region. Thus, our results establish the role of Pyk2 in specific aspects of spatial memory and synaptic plasticity and show the dependence of the phenotype on the type of experiments used to reveal it. In combination with other studies they provide evidence for a selective role of non-receptor tyrosine kinases in specific aspects of hippocampal neurons synaptic plasticity.

scholarly journals Pyk2 in dorsal hippocampus plays a selective role in spatial memory and synaptic plasticity

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Vincenzo Mastrolia ◽  
Omar al Massadi ◽  
Benoit de Pins ◽  
Jean-Antoine Girault
Keyword(s):  
Synaptic Plasticity ◽  
Spatial Memory ◽  
Dorsal Hippocampus ◽  
Training Session ◽  
Long Term Potentiation ◽  
Object Location ◽  
High Frequency Stimulation ◽  
Novel Object ◽  
Ca1 Region

AbstractPyk2 is a Ca2+-activated non-receptor tyrosine kinase enriched in the forebrain, especially in pyramidal neurons of the hippocampus. Previous reports suggested its role in hippocampal synaptic plasticity and spatial memory but with contradictory findings possibly due to experimental conditions. Here we address this issue and show that novel object location, a simple test of spatial memory induced by a single training session, is altered in Pyk2 KO mice and that re-expression of Pyk2 in the dorsal hippocampus corrects this deficit. Bilateral targeted deletion of Pyk2 in dorsal hippocampus CA1 region also alters novel object location. Long term potentiation (LTP) in CA1 is impaired in Pyk2 KO mice using a high frequency stimulation induction protocol but not with a theta burst protocol, explaining differences between previous reports. The same selective LTP alteration is observed in mice with Pyk2 deletion in dorsal hippocampus CA1 region. Thus, our results establish the role of Pyk2 in specific aspects of spatial memory and synaptic plasticity and show the dependence of the phenotype on the type of experiments used to reveal it. In combination with other studies, we provide evidence for a selective role of non-receptor tyrosine kinases in specific aspects of hippocampal neurons synaptic plasticity.

2.45 GHz microwave radiation impairs learning, memory, and hippocampal synaptic plasticity in the rat

2018 ◽  
Vol 34 (12) ◽  
pp. 873-883 ◽  
Author(s):  
Narges Karimi ◽  
Mahnaz Bayat ◽  
Masoud Haghani ◽  
Hamed Fahandezh Saadi ◽  
Gholam Reza Ghazipour
Keyword(s):  
Synaptic Plasticity ◽  
Learning And Memory ◽  
Continuous Wave ◽  
Glutamate Release ◽  
Average Power ◽  
Field Potential ◽  
Long Term Potentiation ◽  
Whole Body ◽  
Spatial Learning And Memory ◽  
Hippocampal Synaptic Plasticity

Microwave (MW) radiation has a close relationship with neurobehavioral disorders. Due to the widespread usage of MW radiation, especially in our homes, it is essential to investigate the direct effect of MW radiation on the central nervous system. Therefore, this study was carried out to determine the effect of MW radiation on memory and hippocampal synaptic plasticity. The rats were exposed to 2.45 GHz MW radiation (continuous wave with overall average power density of 0.016 mW/cm2 and overall average whole-body specific absorption rate value of 0.017 W/kg) for 2 h/day over a period of 40 days. Spatial learning and memory were tested by radial maze and passive avoidance tests. We evaluated the synaptic plasticity and hippocampal neuronal cells number by field potential recording and Giemsa staining, respectively. Our results showed that MW radiation exposure decreased the learning and memory performance that was associated with decrement of long-term potentiation induction and excitability of CA1 neurons. However, MW radiation did not have any effects on short-term plasticity and paired-pulse ratio as a good indirect index for measurement of glutamate release probability. The evaluation of hippocampal morphology indicated that the neuronal density in the hippocampal CA1 area was significantly decreased by MW.

P1-155: Role of the BDNF Val66Met polymorphism in hippocampal synaptic plasticity

2010 ◽  
Vol 6 ◽  
pp. S219-S219
Author(s):  
Ipe Ninan ◽  
Karishma Dagar ◽  
Rosalia Perez-Castro ◽  
Mark R. Plummer ◽  
Francis S. Lee ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
Val66met Polymorphism ◽  
Hippocampal Synaptic Plasticity ◽  
Bdnf Val66met Polymorphism

scholarly journals Promoter-Specific Effects of DREADD Modulation on Hippocampal Synaptic Plasticity and Memory Formation

2016 ◽  
Vol 36 (12) ◽  
pp. 3588-3599 ◽  
Author(s):  
A. J. Lopez ◽  
E. Kramar ◽  
D. P. Matheos ◽  
A. O. White ◽  
J. Kwapis ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
Memory Formation ◽  
Specific Effects ◽  
Hippocampal Synaptic Plasticity

scholarly journals Sex-specific effects of microbiome perturbations on cerebral Aβ amyloidosis and microglia phenotypes

2019 ◽  
Vol 216 (7) ◽  
pp. 1542-1560 ◽  
Author(s):  
Hemraj B. Dodiya ◽  
Thomas Kuntz ◽  
Shabana M. Shaik ◽  
Caroline Baufeld ◽  
Jeffrey Leibowitz ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
Amyloid Β ◽  
Fecal Microbiota ◽  
Male Mice ◽  
Female Mice ◽  
Antibiotic Cocktail ◽  
Specific Effects ◽  
Plaque Pathology ◽  
Microglial Morphology

We demonstrated that an antibiotic cocktail (ABX)-perturbed gut microbiome is associated with reduced amyloid-β (Aβ) plaque pathology and astrogliosis in the male amyloid precursor protein (APP)SWE/presenilin 1 (PS1)ΔE9 transgenic model of Aβ amyloidosis. We now show that in an independent, aggressive APPSWE/PS1L166P (APPPS1-21) mouse model of Aβ amyloidosis, an ABX-perturbed gut microbiome is associated with a reduction in Aβ pathology and alterations in microglial morphology, thus establishing the generality of the phenomenon. Most importantly, these latter alterations occur only in brains of male mice, not in the brains of female mice. Furthermore, ABX treatment lead to alterations in levels of selected microglial expressed transcripts indicative of the “M0” homeostatic state in male but not in female mice. Finally, we found that transplants of fecal microbiota from age-matched APPPS1-21 male mice into ABX-treated APPPS1-21 male restores the gut microbiome and partially restores Aβ pathology and microglial morphology, thus demonstrating a causal role of the microbiome in the modulation of Aβ amyloidosis and microglial physiology in mouse models of Aβ amyloidosis.

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