scholarly journals Effect of pulsed light on postharvest disease control-related metabolomic variation in melon (Cucumis melo) artificially inoculated with Fusarium pallidoroseum

2019 ◽  
Author(s):  
Francisco Oiram Filho ◽  
Ebenézer de Oliveira Silva ◽  
Mônica Maria de Almeida Lopes ◽  
Paulo Riceli Vasconselos Ribeiro ◽  
Andréia Hansen Oster ◽  
...  

AbstractPulsed light, as a postharvest technology, is an alternative to traditional fungicides, and can be used on a wide variety of fruit and vegetables for sanitization or pathogen control. In addition to these applications, other effects also are detected in vegetal cells, including changes in metabolism and production of secondary metabolites, which directly affect disease control response mechanisms. This study aimed to evaluate the possible applications of pulsed ultraviolet light in controlling postharvest rot, mainly caused by Fusarium pallidoroseum in yellow melon ‘Goldex’, in natura, and its implications in the disease control as a function of metabolomic expression to effect fungicidal or fungistatic. The dose of pulsed light (PL) that inhibited F. pallidoroseum growth in melons (Cucumis melo var. Spanish) was 9 KJ m-2. Ultra performance liquid chromatography (UPLC) coupled to a quadrupole time-of-flight (QTOF) mass analyzer identified 12 compounds based on the MS/MS fragmentation patterns. Chemometric analysis by Principal Components Analysis (PCA) and Orthogonal Partial Least Squared Discriminant Analysis (OPLS-DA and S-plot) were used to evaluate the changes in fruit metabolism. PL technology provided protection against postharvest disease in melons, directly inhibiting the growth of F. pallidoroseum through upregulation of specific fruit biomarkers such as pipecolic acid (11), saponarin (7), and orientin (3), which acted as major markers for the defense system against pathogens. PL can thus be proposed as a postharvest technology to avoid chemical fungicides and may be applied to reduce the decay of melon quality during its export and storage.

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0220097 ◽  
Author(s):  
Francisco Oiram Filho ◽  
Ebenézer de Oliveira Silva ◽  
Mônica Maria de Almeida Lopes ◽  
Paulo Riceli Vasconselos Ribeiro ◽  
Andréia Hansen Oster ◽  
...  

2021 ◽  
pp. 79-86
Author(s):  
V. Rangarajan ◽  
W.J. Herbst ◽  
S. Mazibuko ◽  
K.G. Clarke

2017 ◽  
Vol Volume 113 (Number 3/4) ◽  
Author(s):  
Madelien Wooding ◽  
Justin Bradfield ◽  
Vinesh Maharaj ◽  
Dwayne Koot ◽  
Lyn Wadley ◽  
...  

Abstract The antiquity of the use of hunting poisons has received much attention in recent years. In this paper we present the results of a pilot study designed to detect the presence of organic compounds, typically of less than 1200 Da, from poisonous plants that may have been used as hunting poisons in the past. We used ultra-performance liquid chromatography connected to a Synapt G2 high-resolution MS-QTOF mass spectrometer (UPLC-QTOF-MS) to provisionally identify plant-based toxins present in (1) extracts of fresh plant material, (2) a blind control recipe consisting of three plant ingredients and (3) a Hei||om arrow poison of unknown ingredients. Although not all expected toxic compounds were identified, those that were identified compared favourably with those reported in the literature and confirmed through databases, specifically the Dictionary of Natural Products and ChemSpider. MS/MS fragmentation patterns and accurate mass were used for tentative identification of compounds because archaeological residues usually contain insufficient material for unambiguous identification using nuclear magnetic resonance. We highlight the potential of this method for accurately identifying plant-based toxins present on archaeological artefacts and unique (albeit non-toxic) chemical markers that may allow one to infer the presence of toxic plant ingredients in arrow poisons. Any chemical study of archaeological material should consider the unique environmental degradative factors and be sensitive to the oxidative by-products of toxic compounds.


2018 ◽  
Vol 40 (1) ◽  
Author(s):  
Ivan Herman Fischer ◽  
Matheus Froes de Moraes ◽  
Maria Cecília de Arruda Palharini ◽  
Mirian de Souza Fileti ◽  
Juliana Cristina Sodário Cruz ◽  
...  

ABSTRACT Postharvest diseases constitute a serious problem for avocado commercialization. Thus, the present study aimed to evaluate the effect of conventional and alternative products in controlling diseases affecting ‘Hass’ avocados in the field and in the postharvest by carrying out physicochemical characterization of fruits subjected to postharvest treatments. In the field, besides the management adopted by the farmer, seven products were sprayed three times during fruiting for evaluation. Postharvest products were diluted in water or in oxidized polyethylene wax and shellac. Water treatments with potassium phosphite, Soil-Set®, chlorine dioxide, thyme essential oil, sodium bicarbonate, lemon grass essential oil and thiabendazole reduced the incidence of diseased fruits, and anthracnose, the main disease, was controlled with sodium bicarbonate, lemon grass essential oil and thiabendazole. Greater soluble solids content was found for control (water), chlorine dioxide, acibenzolar-S-methyl and thiabendazole. For the products that reduced anthracnose, there was no correlation between the disease and the physicochemical parameters, evidencing that the disease control is not associated with delayed ripening. For wax treatments, diseases were not controlled, and the fruits presented lower titratable acidity with thyme essential oil, sodium bicarbonate, control (wax), acibenzolar-S-methyl and lemon grass essential oil. Control and thyme essential oil were highlighted for maintaining the green coloration of the fruit skin for the shortest period. Under field conditions, azoxystrobin, thiabendazole, difenoconazole+azoxystrobin and acibenzolar-S-methyl+azoxystrobin reduced the occurrence of diseased fruits, while anthracnose control was only obtained with azoxystrobin.


2001 ◽  
pp. 407-412 ◽  
Author(s):  
M. Wisniewski ◽  
C. Wilson ◽  
A. El Ghaouth ◽  
S. Droby

2013 ◽  
pp. 493-498
Author(s):  
Shiping Tian ◽  
Boqiang Li ◽  
Xuequn Shi ◽  
Guozheng Qin ◽  
Yuying Wang

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Guangqiang Huang ◽  
Jie Liang ◽  
Xiaosi Chen ◽  
Jing Lin ◽  
Jinyu Wei ◽  
...  

Chemical constituents from Zhideke granules were rapidly isolated and identified by ultra-performance liquid chromatography (UPLC) coupled with hybrid quadrupole-orbitrap mass spectrometry (MS) in positive and negative ion modes using both full scan and two-stage threshold-triggered mass modes. The secondary fragment ion information of the target compound was selected and compared with the compound reported in databases and related literatures to further confirm the possible compounds. A total of 47 chemical constituents were identified from the ethyl acetate extract of Zhideke granules, including 21 flavonoids and glycosides, 9 organic acids, 4 volatile components, 3 nitrogen-containing compounds, and 10 other compounds according to the fragmentation patterns, relevant literature, and MS data. The result provides a new method for the analysis of chemical constituents of Zhideke granules which laid the foundation for quality control and the study of pharmacodynamic materials of Zhideke granules.


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