scholarly journals Unraveling the hexaploid sweetpotato inheritance using ultra-dense multilocus mapping

2019 ◽  
Author(s):  
Marcelo Mollinari ◽  
Bode A. Olukolu ◽  
Guilherme da S. Pereira ◽  
Awais Khan ◽  
Dorcus Gemenet ◽  
...  
Keyword(s):  
Genetic Map ◽  
Ipomoea Batatas ◽  
Vital Role ◽  
Preferential Pairing ◽  
Genetic Studies ◽  
Inheritance System ◽  
High Ploidy ◽  
Low Levels ◽  
Group 2 ◽  
Integrated Genetic Map

AbstractThe hexaploid sweetpotato (Ipomoea batatas (L.) Lam., 2n = 6x = 90) is an important staple food crop worldwide and has a vital role in alleviating famine in developing countries. Due to its high ploidy level, genetic studies in sweetpotato lag behind major diploid crops significantly. We built an ultra-dense multilocus integrated genetic map and characterized the inheritance system in a sweetpotato full-sib family using our newly implemented software, MAPpoly. The resulting genetic map revealed 96.5% collinearity between I. batatas and its diploid relative I. trifida. We computed the genotypic probabilities across the whole genome for all individuals in the mapping population and inferred their complete hexaploid haplotypes. We provide evidence that most of the meiotic configurations (73.3%) were resolved in bivalents, although a small portion of multivalent signatures (15.7%), among other inconclusive configurations (11.0%) were also observed. Except for low levels of preferential pairing in linkage group 2, we observed a hexasomic inheritance mechanism in all linkage groups. We propose that the hexasomic-bivalent inheritance promotes stability to the allelic transmission in sweetpotato.

scholarly journals Unraveling the Hexaploid Sweetpotato Inheritance Using Ultra-Dense Multilocus Mapping

2019 ◽  
Vol 10 (1) ◽  
pp. 281-292 ◽  
Author(s):  
Marcelo Mollinari ◽  
Bode A. Olukolu ◽  
Guilherme da S. Pereira ◽  
Awais Khan ◽  
Dorcus Gemenet ◽  
...  
Keyword(s):  
Genetic Map ◽  
Ipomoea Batatas ◽  
Vital Role ◽  
Preferential Pairing ◽  
Genetic Studies ◽  
Inheritance System ◽  
High Ploidy ◽  
Low Levels ◽  
Group 2 ◽  
Integrated Genetic Map

The hexaploid sweetpotato (Ipomoea batatas (L.) Lam., 2n = 6x = 90) is an important staple food crop worldwide and plays a vital role in alleviating famine in developing countries. Due to its high ploidy level, genetic studies in sweetpotato lag behind major diploid crops significantly. We built an ultra-dense multilocus integrated genetic map and characterized the inheritance system in a sweetpotato full-sib family using our newly developed software, MAPpoly. The resulting genetic map revealed 96.5% collinearity between I. batatas and its diploid relative I. trifida. We computed the genotypic probabilities across the whole genome for all individuals in the mapping population and inferred their complete hexaploid haplotypes. We provide evidence that most of the meiotic configurations (73.3%) were resolved in bivalents, although a small portion of multivalent signatures (15.7%), among other inconclusive configurations (11.0%), were also observed. Except for low levels of preferential pairing in linkage group 2, we observed a hexasomic inheritance mechanism in all linkage groups. We propose that the hexasomic-bivalent inheritance promotes stability to the allelic transmission in sweetpotato.

Genetic studies of coliphage P1. III. Extended genetic map.

1976 ◽  
Vol 20 (1) ◽  
pp. 177-187 ◽  
Author(s):  
D H Walker ◽  
J T Walker
Keyword(s):  
Genetic Map ◽  
Genetic Studies

scholarly journals An enhanced molecular marker based genetic map of perennial ryegrass (Lolium perenne) reveals comparative relationships with other Poaceae genomes

Genome ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 282-295 ◽  
Author(s):  
Elizabeth S Jones ◽  
Natalia L Mahoney ◽  
Michael D Hayward ◽  
Ian P Armstead ◽  
J Gilbert Jones ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Linkage Map ◽  
Genetic Map ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Genetic Linkage Map ◽  
Population Based ◽  
Genetic Maps ◽  
Linkage Groups ◽  
Integrated Genetic Map

A molecular-marker linkage map has been constructed for perennial ryegrass (Lolium perenne L.) using a one-way pseudo-testcross population based on the mating of a multiple heterozygous individual with a doubled haploid genotype. RFLP, AFLP, isoenzyme, and EST data from four collaborating laboratories within the International Lolium Genome Initiative were combined to produce an integrated genetic map containing 240 loci covering 811 cM on seven linkage groups. The map contained 124 codominant markers, of which 109 were heterologous anchor RFLP probes from wheat, barley, oat, and rice, allowing comparative relationships between perennial ryegrass and other Poaceae species to be inferred. The genetic maps of perennial ryegrass and the Triticeae cereals are highly conserved in terms of synteny and colinearity. This observation was supported by the general agreement of the syntenic relationships between perennial ryegrass, oat, and rice and those between the Triticeae and these species. A lower level of synteny and colinearity was observed between perennial ryegrass and oat compared with the Triticeae, despite the closer taxonomic affinity between these species. It is proposed that the linkage groups of perennial ryegrass be numbered in accordance with these syntenic relationships, to correspond to the homoeologous groups of the Triticeae cereals.Key words: Lolium perenne, genetic linkage map, RFLP, AFLP, conserved synteny.

Breeding and Mating Systems of Australian Proteaceae

1998 ◽  
Vol 46 (4) ◽  
pp. 421 ◽  
Author(s):  
Ross L. Goldingay ◽  
Susan M. Carthew
Keyword(s):  
Fruit Development ◽  
Mating Systems ◽  
Pollen Deposition ◽  
Genetic Studies ◽  
The Past ◽  
Wide Range ◽  
The Family ◽  
Self Compatibility ◽  
Low Levels ◽  
Key Events

There has been a significant increase in the number of studies investigating plant breeding and mating systems over the past 10 years. The family Proteaceae, in particular, has dominated such research conducted in Australia. Thus it is now timely to present a critical review of the breeding and mating systems of the Australian Proteaceae. It is hoped that this will stimulate further research. The review covers key events between pollen deposition on stigmas through to fruit set. The genus Banksia, although not the most diverse of the family, has received a disproportionate amount of attention. It has featured in nine published studies of self-compatibility compared to 13 studies spanning the other 45 genera and has featured in eight genetic studies of the mating system compared to just two on other genera. Few studies have assessed the timing of stigma receptivity despite the intriguing situation in most Proteaceae of auto-deposition of self-pollen on or near stigmas at anthesis. Studies suggest that stigmas are not receptive until 0.5–4 days after anthesis. Banksia species appear to show low levels of self-compatibility although one subspecies shows high levels of selfing and evidence of selective fruit development. Self-compatibility may be more common in other genera, although a dearth of studies precludes generalisation. Assessment of mating systems indicates almost complete outcrossing for most species, lending support to the idea of selective fruit development. It is clear that many further studies of all topics are required but particularly across a wide range of genera because many have not been studied at all.

scholarly journals Low Vitamin C Status in Patients with Cancer Is Associated with Patient and Tumor Characteristics

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2338 ◽  
Author(s):  
Rebecca White ◽  
Maria Nonis ◽  
John F. Pearson ◽  
Eleanor Burgess ◽  
Helen R. Morrin ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Vitamin C ◽  
Human Health ◽  
Cancer Patients ◽  
Vital Role ◽  
Tumor Characteristics ◽  
Patients With Cancer ◽  
Plasma Ascorbate ◽  
Number Of Patients ◽  
Low Levels

Vitamin C (ascorbate) acts as an antioxidant and enzyme cofactor, and plays a vital role in human health. Vitamin C status can be affected by illness, with low levels being associated with disease due to accelerated turnover. However, robust data on the ascorbate status of patients with cancer are sparse. This study aimed to accurately measure ascorbate concentrations in plasma from patients with cancer, and determine associations with patient or tumor characteristics. We recruited 150 fasting patients with cancer (of 199 total recruited) from two cohorts, either prior to cancer surgery or during cancer chemo- or immunotherapy. A significant number of patients with cancer had inadequate plasma ascorbate concentrations. Low plasma status was more prevalent in patients undergoing cancer therapy. Ascorbate status was higher in women than in men, and exercising patients had higher levels than sedentary patients. Our study may prompt increased vigilance of ascorbate status in cancer patients.

scholarly journals A physical map of the papaya genome with integrated genetic map and genome sequence

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 371 ◽  
Author(s):  
Qingyi Yu ◽  
Eric Tong ◽  
Rachel L Skelton ◽  
John E Bowers ◽  
Meghan R Jones ◽  
...  
Keyword(s):  
Genetic Map ◽  
Genome Sequence ◽  
Physical Map ◽  
Integrated Genetic Map

Integrated genetic map of human chromosome 2

1995 ◽  
Vol 59 (4) ◽  
pp. 413-434 ◽  
Author(s):  
S. COX ◽  
S. P. BRYANT ◽  
A. COLLINS ◽  
J. WEISSENBACH ◽  
H. DONIS-KELLER ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Genetic Map ◽  
Chromosome 2 ◽  
Integrated Genetic Map

scholarly journals Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

2020 ◽  
Author(s):  
Digang Zeng ◽  
Chunling Yang ◽  
Qiangyong Li ◽  
Weilin Zhu ◽  
Xiuli Chen ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Genetic Map ◽  
Litopenaeus Vannamei ◽  
Quantitative Trait ◽  
Pacific White Shrimp ◽  
High Density ◽  
White Shrimp ◽  
Genetic Studies ◽  
The Pacific ◽  
Ammonia Tolerance

Abstract Background: Ammonia is one of the most common toxicological environment factors affecting shrimp health. Although ammonia tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available. Results: In this study, we constructed a high-density genetic map of the Pacific white shrimp (Litopenaeus vannamei) using specific length amplified fragment sequencing (SLAF-seq). The constructed genetic map contained 17,338 polymorphic markers spanning 44 linkage groups, with a total distance of 6,360.12 centimorgans (cM) and an average distance of 0.37 cM. Using this genetic map, we identified a quantitative trait locus (QTL) that explained 7.41–8.46% of the phenotypic variance in L. vannamei survival time under acute ammonia stress. We then sequenced the transcriptomes of the most ammonia-tolerant and the most ammonia-sensitive individuals from each of four genetically distinct L. vannamei families. We found that 7546 genes were differentially expressed between the ammonia-tolerant and ammonia-sensitive individuals. Using QTL analysis and the transcriptomes, we identified one candidate gene (annotated as an ATP synthase g subunit) associated with ammonia tolerance. Conclusions: In this study, we constructed a high-density genetic map of L. vannamei and identified a QTL for ammonia tolerance. By combining QTL and transcriptome analyses, we identified a candidate gene associated with ammonia tolerance. Our work provides the basis for future genetic studies focused on molecular marker-assisted selective breeding.

scholarly journals Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

2020 ◽  
Author(s):  
Digang Zeng ◽  
Chunling Yang ◽  
Qiangyong Li ◽  
Weilin Zhu ◽  
Xiuli Chen ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Genetic Map ◽  
Litopenaeus Vannamei ◽  
Quantitative Trait ◽  
Pacific White Shrimp ◽  
High Density ◽  
White Shrimp ◽  
Genetic Studies ◽  
The Pacific ◽  
Ammonia Tolerance

Abstract Background: Ammonia is one of the most common toxicological environment factors affecting shrimp health. Although ammonia tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available. Results: In this study, we constructed a high-density genetic map of the Pacific white shrimp (Litopenaeus vannamei) using specific length amplified fragment sequencing (SLAF-seq). The constructed genetic map contained 17,338 polymorphic markers spanning 44 linkage groups, with a total distance of 6,360.12 centimorgans (cM) and an average distance of 0.37 cM. Using this genetic map, we identified a quantitative trait locus (QTL) that explained 7.41–8.46% of the phenotypic variance in L. vannamei survival time under acute ammonia stress. We then sequenced the transcriptomes of the most ammonia-tolerant and the most ammonia-sensitive individuals from each of four genetically distinct L. vannamei families. We found that 7546 genes were differentially expressed between the ammonia-tolerant and ammonia-sensitive individuals. Using QTL analysis and the transcriptomes, we identified one candidate gene (annotated as an ATP synthase g subunit) associated with ammonia tolerance. Conclusions: In this study, we constructed a high-density genetic map of L. vannamei and identified a QTL for ammonia tolerance. By combining QTL and transcriptome analyses, we identified a candidate gene associated with ammonia tolerance. Our work provides the basis for future genetic studies focused on molecular marker-assisted selective breeding.

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